FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003674806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTAGACAGATCAACGAGACAAAAAGTCAACAAGGATACCCAGGAATT29.090909090909092No Hit
GATAGTGCCACCGCACTCCAGCCTGGGCAACAGAGCAAGCTTATGTCTCA29.090909090909092No Hit
GACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATC29.090909090909092No Hit
CCCATGGGTGTCCGGACCGAGCCACGGATCGCCCACTTCGACCCAGCGCC29.090909090909092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCTGTC29.090909090909092Clontech Universal Primer Mix Long (95% over 21bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT14.545454545454546No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATG14.545454545454546No Hit
GCCCGCTGGTGGCCCAGGCGCAGGCCCTAGGAGCCGCCTACCAAAAAGCC14.545454545454546No Hit
CTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAAGTAA14.545454545454546No Hit
AAATATTCGCAACCTACTCGTCTGACAAAGGGCTAATATCCAGAATCTAC14.545454545454546No Hit
TGCCTGTAATCCCAGCACTTTCGGGAGGCAAAGATGTTAGTGACATACTT14.545454545454546No Hit
GAGGAAGACCGCCAGAAAACAGAAAATGTTTCAACGTGCGCAAGAGTTGC14.545454545454546No Hit
CAGTGTAATTATGGGGATAAAGGCCAGATTGCAAATGGGTTAAAAGTTAT14.545454545454546No Hit
GCAATAAACCATTCATGAGGGATCTGCCCCCATGATCCAAACACCCCCAA14.545454545454546No Hit
GAGTTACTCATAATATTCGCTTTTTGCCTTTTAACTGTCTGATCTATAGA14.545454545454546No Hit
GTGCGGGCGTCGTCGAGCACGCCGAGGCGGTCCATCCGGTCGCGGGCGTC14.545454545454546No Hit
CCCATAGCCCCTGCACCAGCCGTCGGAGGAGTTTGCTGGACGCGACCTGT14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)