FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003674807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGTATAACATTTTCTTTATCCACTCACTGATTGATGAACATTTGG211.11111111111111No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA211.11111111111111Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGATGACCCCAATACGCAAAATTAACCCCC211.11111111111111No Hit
GTAATAGACAAGACTGAATCAGAGTGGCAGAATGTGGAGGCATAATGAAT211.11111111111111No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGTACAGTGACTGTCTCTTAT15.555555555555555No Hit
ATAGATATCATTCTCTACGATTTTTCTCTCAAACATACGGTACAAATCCT15.555555555555555No Hit
ACCACTCGACTACCCCGTTTTCTTTGGCGCGAACTACGGATGCGCGATGG15.555555555555555No Hit
GAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT15.555555555555555Clontech SMART CDS Primer II A (100% over 26bp)
CCCTCTGTTCACCATTGACCTCAGATAGGAATTTTTTTTTTTTTTGAGAC15.555555555555555No Hit
CGCCAGGGACCGTCCTGCAGACCGGCGGTGGTGAGCCGCTAGTCCTGTCT15.555555555555555No Hit
GCCATCTGTACAACCCTCTTTTCATCTACGGCGGCGTCGGCCTGGGCAAG15.555555555555555No Hit
GAACAGGCCAATAACAAGCCGCAAGATTGAAATGGTAATTAAAACATTAC15.555555555555555No Hit
CGCCATGGTTTCCAATCAATGGCACTGGGAAACTGGACTTTCAAAGCAAC15.555555555555555No Hit
TCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGC15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)