FastQCFastQC Report
Tue 10 May 2022
EGAF00003674909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464070
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC25870.5574590040295645No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC24480.5275066261555369No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT21380.4607063589544681No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20020.4314004352791605No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC18170.3915357596914259No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG17350.37386601159307864No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA16300.3512401146378779No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA15860.3417587863899843No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC15520.3344323054711574No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA14040.30254056500096965No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT13290.2863792100329692No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT12720.2740965802572888No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC11970.25793522528928825No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA11830.25491843902859485No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA11610.250177774904648No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC11140.24004999245803438No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG11080.23875708406059432No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10850.23380093520374082No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA10220.22022539703062038No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT9930.21397633977632682No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG9860.21246794664598012No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9640.20772728252203332No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA9430.20320210313099318No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG9260.19953886267157972No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG9050.19501368328053958No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT8880.19135044282112612No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT8650.18639429396427265No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG8530.18380847716939255No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT8200.17669748098347232No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG8170.17605102678475232No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC8060.1736806947227789No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA7900.17023293899627212No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT7760.1672161527355787No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT7680.1654922748723253No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT7530.1622600038787252No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG7520.16204451914581852No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA7460.16075161074837846No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA7420.1598896718167518No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC7370.1588122481522184No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC7290.15708837028896502No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT6910.14889995043851142No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA6850.1476070420410714No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG6840.14739155730816472No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA6760.14566767944491132No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT6670.14372831684875126No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC6630.14286637791712456No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG6630.14286637791712456No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG6570.14157346951968452No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA6540.1409270153209645No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA6530.14071153058805783No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA6440.13877216799189776No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC6390.1376947443273644No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG6320.1361863511970177No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG6270.13510892753248432No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA6170.1329540802034176No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG6160.13273859547051092No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC6130.1320921412717909No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC6120.13187665653888422No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA6100.1314456870730709No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG6090.1312302023401642No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG6020.1297218092098175No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT5960.12842890081237746No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA5950.12821341607947076No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT5870.1264895382162174No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT5840.12584308401749736No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA5790.12476566035296399No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA5780.12455017562005732No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG5700.12282629775680393No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC5680.12239532829099059No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC5630.12131790462645722No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT5610.12088693516064386No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA5570.12002499622901719No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG5490.11830111836576378No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG5440.11722369470123042No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT5430.11700820996832374No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT5400.11636175576960371No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC5390.11614627103669706No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA5340.11506884737216368No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC5310.11442239317344366No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT5270.11356045424181697No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC5230.11269851531019028No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG5220.1124830305772836No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC5190.11183657637856358No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC5120.11032818324821687No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT5110.11011269851531019No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT5090.10968172904949686No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG5070.1092507595836835No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG5040.10860430538496346No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA5000.10774236645333679No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT4920.10601848859008338No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT4920.10601848859008338No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA4910.10580300385717673No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG4900.10558751912427004No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG4890.10537203439136336No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA4880.10515654965845671No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC4870.10494106492555003No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT4860.10472558019264334No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC4850.10451009545973669No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA4840.10429461072683001No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG4830.10407912599392333No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA4790.10321718706229664No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC4780.10300170232938996No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC4770.10278621759648328No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG4770.10278621759648328No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG4700.10127782446613658No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG4690.1010623397332299No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT4690.1010623397332299No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG4670.10063137026741656No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC4660.10041588553450988No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC4650.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)