FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003675180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGATGCTGTCCAACGTCGAACACCTTGACCACGCGCTTCAGACGGTG37.142857142857142No Hit
GTCCGATAACGATCGCCATTGGCTGATGGAACTCGTCGCCCGCGTTGAGC24.761904761904762No Hit
ACTGACCGATGAGTGGCCGCAATATCCCGGTGACGAGATAAAACAAATCG24.761904761904762No Hit
CCAAGCGCGTCTATGAATCCGAGAAAACGTATAGGATCCTTCCAAACGAG24.761904761904762No Hit
GTTCGTCCTCGCCATTGTGGTGACGGCGATCATTGGATCGATGCTTCCTG24.761904761904762No Hit
GGCATAGAGCAGGACAAGTAAGACCGTGCCGAGGCCAGCCGTCATTTCGG12.380952380952381No Hit
CTCCACAGCTGTGTTCCATTGCGCCAGTGCTCAATATTTTGCCATAATAA12.380952380952381No Hit
CGATCTGCGCGCGCCGCTCGTCAACGTGATGGGCTTCACCAGCGAGCTGG12.380952380952381No Hit
TCACCAGATCCTGGCCTCGAACATCGCCAACGCCGATACGCCCAACTACA12.380952380952381No Hit
AAAGCTGGTCGGCCCGTAGCGGTGCGTTTCCGAACCGCCGGTCCGAGACC12.380952380952381No Hit
GCCGACTTCATCGTGCTGGACCCGGCAGCCACGCCGCTGCTGGCCCGCCG12.380952380952381No Hit
CACCCGCCTCCCCCACACCCCGCATCCGGTGTGTCTCCGCCTGGCCCGGC12.380952380952381No Hit
GCCGTGGTAGACGACCGGCACGCCGCGCGCTGTGTCGGTGACAATAAAGC12.380952380952381No Hit
GGGAAAATGTCAGATGATTTGTTGAAGTCGGCTGCTTTAGGAAATCATTT12.380952380952381No Hit
CGTGCGTCCCGCGCAATTGACAATGACTTGCGTGCTCGGGTCCGGTGCAA12.380952380952381No Hit
GCCCAGCAACGCGGCATGCGCATGATCACGGGCAAGGTGATGATGGACCG12.380952380952381No Hit
GATCAAGAAACAGCAGCCCCAGCGATGTTAGTCCGCTTGCGCCGCCAACC12.380952380952381No Hit
GGTTCTGATCGTGGTTTATCCACCAGCGTTGGCGTCAGCTATGAACTCGA12.380952380952381No Hit
GCGTCGGATCGTCTTGGAGAACTCTACGGTTTCGAGCAGCGCCCGCTTCG12.380952380952381No Hit
GCGCGGGGCGTGCGCATCTCGCTCGATGATTTCGGCATCGGATTCTCGTC12.380952380952381No Hit
GTGATCATCCTTGGGCAGAACACCAAGGCACTGTTTGCACCGGTGGACAA12.380952380952381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.380952380952381No Hit
CAGGGAAGGAAATGGTGATGCATTGTCAAGCTGGCCACAGTAAAACATGA12.380952380952381No Hit
ATCACGGACAGGAAACAAGCTGAACTGCTATTGCAGGAAGCCCGCTTGCG12.380952380952381No Hit
GGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCG12.380952380952381No Hit
GACCACTAAAAACAATGCGCCGACCAGAATACATTCGTAAACACCATTAA12.380952380952381No Hit
ATGCTGCCGAGTCCATCAAGAATCGCGTCGGCCGCCACCTCGACCATTAC12.380952380952381No Hit
CTCTGACATCGAGGCGACGGTTCGCGAGCTGCGGCAGCGCGGGGTGACGT12.380952380952381No Hit
CTCTCCTGGTGTGGATGAGTGTGTTCACCAGTGCAGAAGCTTTCCAAATC12.380952380952381No Hit
GCTCGACACAACAGCAGGCGTTTATCGGCGTGTTTGTCTTTATGATGCCC12.380952380952381No Hit
GGTGTAATCCCAGCTCGAAATGGGGACCTTGCGTGAGGGCTGTACGTAAA12.380952380952381No Hit
GTACAGGGTGGCAAGGATGCCGTTGATGCGCTGCTCGAGCATCCGTATGT12.380952380952381No Hit
ACGCCGATGTGTTCCTCTGCGAGGCCGCCTTCGAGGACGGCCGGGACGAT12.380952380952381No Hit
AGGCAAGAGGACTCGATGTACGCGCGAGTGCCGTCCGTCGAGGCGCCATC12.380952380952381No Hit
ATGTTCGACGCCGTGATCGCGGTGAACCTGAAGGGCGTGTTCCTCGGACT12.380952380952381No Hit
ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCA12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)