FastQCFastQC Report
Tue 10 May 2022
EGAF00003675765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705447
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC55540.3256624216407781No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT43940.25764506314180385No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG36200.21226106703990216No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT33880.19865759534010732No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT33160.19443582826086064No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT29900.17532060509649375No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG28470.16693570659187884No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT25820.15139725831409595No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG25770.15110408004470383No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT23650.1386733214224775No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC22730.1332788412656623No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT22620.13263384907299963No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA22410.13140250034155268No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC21560.12641846976188648No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT20880.12243124529815351No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC20390.11955809825811062No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT20050.11756448602624414No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC19480.11422225375517386No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG19350.11345999025475433No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC18310.10736188225139802No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG18200.10671689005873533No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG17520.10272966559500236No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG17520.10272966559500236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)