FastQCFastQC Report
Tue 10 May 2022
EGAF00003675768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625428
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC46070.2834330404053579No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT38650.23778352532379163No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT34810.21415897843521828No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG30060.18493590611211322No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT27520.16930925270144231No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT25840.15897351343769148No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT23960.1474073290234941No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT23480.14445426066242245No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG23100.14211641487657406No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC21450.13196524238539017No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG21340.1312884975526446No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA20910.12864304047918454No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC19710.12126036957650539No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT19310.11879947927561232No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC19120.11763055638268813No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG18840.11590793317206298No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT18180.11184746417558944No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT17570.10809460646672754No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG17300.10643350551362471No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC17160.10557219390831216No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC17040.10483392681804424No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG16380.10077345782157068No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG16260.10003519073130276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)