FastQCFastQC Report
Tue 10 May 2022
EGAF00003675779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45711
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT1770.38721533110192297No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1620.3544004725339634No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1210.2647065257815405No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG990.21657806654853318No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG960.21001509483494127No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG960.21001509483494127No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA880.1925138369320295No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG860.18813852245630155No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT860.18813852245630155No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT830.18157555074270962No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA830.18157555074270962No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG790.17282492179125375No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG770.1684496073155258No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT750.16407429283979785No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT750.16407429283979785No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG740.1618866356019339No Hit
GTACATGGGAGAATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTT730.15969897836406993No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT680.14876069217475008No Hit
ACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGT660.14438537769902213No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA650.14219772046115814No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG650.14219772046115814No Hit
GTATGGAGGAGATGCGGCTCATGGAGAAGCATCCAGGCTCAGGTGACCTT640.14001006322329418No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT640.14001006322329418No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT640.14001006322329418No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT610.13344709150970227No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG580.12688411979611033No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA570.12469646255824637No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT570.12469646255824637No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT570.12469646255824637No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC570.12469646255824637No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC560.1225088053203824No Hit
GTATCAACGCAGAGTACATGGGAAGATTCCATGCCTAGATGTGATACACG550.12032114808251844No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA550.12032114808251844No Hit
GGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG550.12032114808251844No Hit
ATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTTTTGTTTTTGTTT540.11813349084465447No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT540.11813349084465447No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT530.1159458336067905No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA520.11375817636892652No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG520.11375817636892652No Hit
CATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGA520.11375817636892652No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA520.11375817636892652No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC520.11375817636892652No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA510.11157051913106254No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC510.11157051913106254No Hit
ATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAA510.11157051913106254No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC500.10938286189319858No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA490.1071952046553346No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG490.1071952046553346No Hit
GTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTGGTGAG490.1071952046553346No Hit
ATATTACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATC480.10500754741747063No Hit
TTGTAGGACTCAGCCAGACGTGGAGGGAGCCGGGTGCCGCAGGAAGTCCT480.10500754741747063No Hit
TTGATGTACAGGGTGAAGGAACTGTAACGGCAGCTGATATTACACACGAT480.10500754741747063No Hit
CTCTTGAACCGTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAA480.10500754741747063No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA480.10500754741747063No Hit
ATTCTATCTATACGCCAGTTTCCCGTGTATCTTATCAGGTAGAGAACACT470.10281989017960666No Hit
GTCCTGAGTATGGAGGAGATGCGGCTCATGGAGAAGCATCCAGGCTCAGG470.10281989017960666No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC460.1006322329417427No Hit
GTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGAA460.1006322329417427No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC460.1006322329417427No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA460.1006322329417427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)