FastQCFastQC Report
Tue 10 May 2022
EGAF00003675780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45711
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1850.4047165890048347No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT1480.3237732712038678No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1170.2559558968300847No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG1030.22532869549998905No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA970.2122027520728052No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT900.19688915140775742No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG830.18157555074270962No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT750.16407429283979785No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG740.1618866356019339No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG730.15969897836406993No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG730.15969897836406993No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT720.15751132112620594No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT700.153136006650478No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA690.15094834941261404No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT680.14876069217475008No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA650.14219772046115814No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT650.14219772046115814No Hit
GTATCAACGCAGAGTACATGGGAGGACGTGGACCATTACTGTCCCAACTG650.14219772046115814No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG640.14001006322329418No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA640.14001006322329418No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG620.13563474874756623No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT610.13344709150970227No Hit
CATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGA590.12907177703397432No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT570.12469646255824637No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG560.1225088053203824No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT560.1225088053203824No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT550.12032114808251844No Hit
GTACATGGGGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGGCT540.11813349084465447No Hit
ATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTTTTGTTTTTGTTT530.1159458336067905No Hit
TTGTAGGACTCAGCCAGACGTGGAGGGAGCCGGGTGCCGCAGGAAGTCCT530.1159458336067905No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG530.1159458336067905No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA530.1159458336067905No Hit
ATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAA520.11375817636892652No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG520.11375817636892652No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT500.10938286189319858Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA500.10938286189319858No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA500.10938286189319858No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG500.10938286189319858No Hit
ACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGT500.10938286189319858No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA490.1071952046553346No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA480.10500754741747063No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG480.10500754741747063No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT480.10500754741747063No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC480.10500754741747063No Hit
GCGGAGAGGAGTCCTGAGTATGGAGGAGATGCGGCTCATGGAGAAGCATC480.10500754741747063No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC470.10281989017960666No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA470.10281989017960666No Hit
CTGTAAGGCAAGATCTTTGTCATCAGGGACGGGAAGAGACGGATAAGATA470.10281989017960666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA470.10281989017960666No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC460.1006322329417427No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC460.1006322329417427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)