FastQCFastQC Report
Tue 10 May 2022
EGAF00003675781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44880
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1820.4055258467023173No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT1660.3698752228163993No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG1140.2540106951871658No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1060.23618538324420676No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT1000.22281639928698754No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG990.22058823529411764No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA940.20944741532976824No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG830.18493761140819964No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG790.17602495543672014No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC770.1715686274509804No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT750.16711229946524064No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA750.16711229946524064No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT700.15597147950089127No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA670.14928698752228164No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT670.14928698752228164No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA660.14705882352941177No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA650.1448306595365419No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT650.1448306595365419No Hit
CATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGA640.14260249554367202No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT620.13814616755793227No Hit
ATATTACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATC590.13146167557932265No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG590.13146167557932265No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG580.12923351158645274No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT580.12923351158645274No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG570.1270053475935829No Hit
GTATCAACGCAGAGTACATGGGAGGACGTGGACCATTACTGTCCCAACTG560.12477718360071302No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA550.12254901960784313No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG550.12254901960784313No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG530.11809269162210338No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT530.11809269162210338No Hit
TTGATGTACAGGGTGAAGGAACTGTAACGGCAGCTGATATTACACACGAT530.11809269162210338No Hit
CTGTAAGGCAAGATCTTTGTCATCAGGGACGGGAAGAGACGGATAAGATA520.11586452762923352No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC520.11586452762923352No Hit
GTACATGGGAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTG520.11586452762923352No Hit
GCGGAGAGGAGTCCTGAGTATGGAGGAGATGCGGCTCATGGAGAAGCATC520.11586452762923352No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA510.11363636363636363No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTTAGCATC510.11363636363636363No Hit
ACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGT510.11363636363636363No Hit
GTATCAACGCAGAGTACATGGGAAGATTCCATGCCTAGATGTGATACACG500.11140819964349377No Hit
TTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGA500.11140819964349377No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT500.11140819964349377No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA500.11140819964349377No Hit
CATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTC490.10918003565062388No Hit
ATGTTACAACGATTATCTTACACATTAAAAAGCTTGCATTGAAAATCTAC490.10918003565062388No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA480.10695187165775401No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA480.10695187165775401No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG480.10695187165775401No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA480.10695187165775401No Hit
GATGAAGAGAAGACGCTAGAAATTGATGTACAGGGTGAAGGAACTGTAAC480.10695187165775401No Hit
GTACATGGGAGAATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTT470.10472370766488412No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG470.10472370766488412No Hit
GGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG470.10472370766488412No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC460.10249554367201426No Hit
GTATGGAGGAGATGCGGCTCATGGAGAAGCATCCAGGCTCAGGTGACCTT450.1002673796791444No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA450.1002673796791444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)