FastQCFastQC Report
Tue 10 May 2022
EGAF00003675782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44880
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1680.37433155080213903No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT1590.3542780748663102No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC970.21613190730837792No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG950.21167557932263814No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG930.20721925133689842No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG830.18493761140819964No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG810.1804812834224599No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG780.17379679144385027No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT750.16711229946524064No Hit
GTACATGGGAGAATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTT740.16488413547237077No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT740.16488413547237077No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT730.1626559714795009No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA710.15819964349376114No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG670.14928698752228164No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG660.14705882352941177No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA660.14705882352941177No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA650.1448306595365419No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG650.1448306595365419No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT620.13814616755793227No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA620.13814616755793227No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT590.13146167557932265No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT590.13146167557932265No Hit
GTATCAACGCAGAGTACATGGGAGGACGTGGACCATTACTGTCCCAACTG590.13146167557932265No Hit
GTACATGGGACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG580.12923351158645274No Hit
CATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGA570.1270053475935829No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT560.12477718360071302No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC550.12254901960784313No Hit
GTACATGGGGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGGCT550.12254901960784313No Hit
GGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG550.12254901960784313No Hit
ATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTTTTGTTTTTGTTT540.12032085561497327No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA530.11809269162210338No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC520.11586452762923352No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT510.11363636363636363No Hit
ATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTGGT500.11140819964349377No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT500.11140819964349377No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC490.10918003565062388No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT490.10918003565062388No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG490.10918003565062388No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC480.10695187165775401No Hit
ATTCTATCTATACGCCAGTTTCCCGTGTATCTTATCAGGTAGAGAACACT480.10695187165775401No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA470.10472370766488412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATC460.10249554367201426No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA450.1002673796791444No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC450.1002673796791444No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA450.1002673796791444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)