FastQCFastQC Report
Tue 10 May 2022
EGAF00003675913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19885
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA19369.735981895901432No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT14227.151118933869752No Hit
GTACATGGGCTTCAGCCTGCGCCTGCGCAATGCCGAGAAGTCGGTGACCG520.2615036459642947No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA510.2564747296957505No Hit
GTACATGGGTGTCCATCGTGAATCCGGACCAGTACAACCGATACAAGCAA510.2564747296957505No Hit
GTACATGGGAGGGCAGCAGGGTGGCTGCGACCACGATGGACGCCGCCACC460.23133014835302995No Hit
GTACATGGGGCCGCCGAGCGCCAGCCCCTGCGCGAAGCGCAGGACCGCAA390.19612773447322102No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA360.18104098566758864No Hit
GTACATGGGGACGTGGCCGCGAGGATGACGGCCGAGGGCATTCCCGACAC350.17601206939904449No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT350.17601206939904449No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA340.1709831531305004No Hit
GTATCAACGCAGAGTACATGGGCTTCAGCCTGCGCCTGCGCAATGCCGAG340.1709831531305004No Hit
GTACATGGGAAGCCTGGGGATCCCCGCATGGACCGCGGCTTCCGCCGGGT340.1709831531305004No Hit
GCTTCAGCCTGCGCCTGCGCAATGCCGAGAAGTCGGTGACCGACGAGCGC320.16092532059341214No Hit
GTACATGGGCGTCGATCATCTGGCCGTGCTGCAGCGTGTGGCGGTGGTCG300.15086748805632386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.15086748805632386No Hit
GTACATGGGAGCGTGGTCAGCGTCATCGACCAGTGGTAGAGCGCGAGGTT290.14583857178777973No Hit
ACGCAGAGTACATGGGCTTCAGCCTGCGCCTGCGCAATGCCGAGAAGTCG290.14583857178777973No Hit
TATCAACGCAGAGTACATGGGCTTCAGCCTGCGCCTGCGCAATGCCGAGA290.14583857178777973No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC270.13578073925069148No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTT260.13075182298214735No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT260.13075182298214735No Hit
CACACACACACACTCACACACACACACACACACACACACACACACACACA250.1257229067136032No Hit
GTACATGGGGAGCAAGACGGGGACGGGGTGCGACTCATGCAGCCCTCGCC240.1206939904450591No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC230.11566507417651498No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA220.11063615790797084No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT220.11063615790797084No Hit
CACACACACACACACACACACACACACACACACACACACACACACAAACA220.11063615790797084No Hit
GTATCAACGCAGAGTACATGGGGCCGCCGAGCGCCAGCCCCTGCGCGAAG210.10560724163942671No Hit
TTCTTAGCCTTCCCACTCCCGCCCTCTCTCAAGCTCCGGTGCCCACAAGC200.10057832537088257No Hit
GTACATGGGCACACACACACACACACACACACACACACACACACACACAC200.10057832537088257No Hit
GTACATGGGCCAGCTTCCCGACCGAGGACGGCCTCGTGCGCTCGGTGGCC200.10057832537088257No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG200.10057832537088257No Hit
GAGTACATGGGCTTCAGCCTGCGCCTGCGCAATGCCGAGAAGTCGGTGAC200.10057832537088257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)