FastQCFastQC Report
Tue 10 May 2022
EGAF00003675931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50600
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1590.31422924901185767No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1440.2845849802371541No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1410.27865612648221344No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1310.25889328063241107No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1170.23122529644268777No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1140.22529644268774707No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1140.22529644268774707No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC1000.1976284584980237No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA990.1956521739130435No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC950.18774703557312253No Hit
CCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAG930.18379446640316205No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG930.18379446640316205No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA910.1798418972332016No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC910.1798418972332016No Hit
GTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGG910.1798418972332016No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT850.16798418972332016No Hit
GTATTAGCTCCATGACCCAGGCTGTTGACGTCTGCCCGGGCTGAAGTCAC810.1600790513833992No Hit
TGATAAAGTAGCATGAATGTGAAGCTCTTTGCTTCCTGGCCCCTAAAACA800.15810276679841898No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT790.15612648221343875No Hit
GTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA760.15019762845849802No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG760.15019762845849802No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC760.15019762845849802No Hit
GTACATGGGTCGCCCAGCCTGGAGTGCAGTGGTGCACACAATCTCAGCTC740.14624505928853754No Hit
GAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAG720.14229249011857706No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA700.1383399209486166No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT700.1383399209486166No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT680.13438735177865613No Hit
GTACATGGGGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATG670.1324110671936759No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC670.1324110671936759No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG660.13043478260869568No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG630.12450592885375494No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG630.12450592885375494No Hit
CTATTGGCCAGGCTGATCTCGAACTCCCGACCTCAGGTGATCCACCTGCC620.1225296442687747No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT620.1225296442687747No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG620.1225296442687747No Hit
GTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTTGCTTTTTTTTTTT620.1225296442687747No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG620.1225296442687747No Hit
GTTTAATCCTGTGGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGG610.12055335968379446No Hit
GTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGGGACCTGCTGCAGG600.11857707509881424No Hit
GTATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGA600.11857707509881424No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC590.11660079051383399No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT570.11264822134387353No Hit
GTACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT560.11067193675889328No Hit
GGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGGCCCTTGCTACTC560.11067193675889328No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT540.10671936758893281No Hit
ATATGTATGTATACCTTGGATGCAATTCCTTCTTTCCTGGGGAGGCAAGC540.10671936758893281No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT540.10671936758893281No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG540.10671936758893281No Hit
CCCATATACTCCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCC530.10474308300395256No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG530.10474308300395256No Hit
GTACAGATGCAAAGTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGA530.10474308300395256No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG530.10474308300395256No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG530.10474308300395256No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT520.10276679841897234No Hit
TATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAA510.1007905138339921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)