FastQCFastQC Report
Tue 10 May 2022
EGAF00003675932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50600
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1420.28063241106719367No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1400.2766798418972332No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1320.26086956521739135No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1290.2549407114624506No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1190.23517786561264822No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1070.21146245059288538No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1040.20553359683794467No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1020.2015810276679842No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC940.1857707509881423No Hit
CTATTGGCCAGGCTGATCTCGAACTCCCGACCTCAGGTGATCCACCTGCC880.17391304347826086No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC860.16996047430830039No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT810.1600790513833992No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT790.15612648221343875No Hit
TGATAAAGTAGCATGAATGTGAAGCTCTTTGCTTCCTGGCCCCTAAAACA790.15612648221343875No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG780.1541501976284585No Hit
CCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAG770.15217391304347827No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT770.15217391304347827No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG770.15217391304347827No Hit
GTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGG750.14822134387351776No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG720.14229249011857706No Hit
GTACATGGGTCGCCCAGCCTGGAGTGCAGTGGTGCACACAATCTCAGCTC710.14031620553359683No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG690.13636363636363638No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG660.13043478260869568No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC660.13043478260869568No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC660.13043478260869568No Hit
GTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA650.12845849802371542No Hit
GTATTAGCTCCATGACCCAGGCTGTTGACGTCTGCCCGGGCTGAAGTCAC650.12845849802371542No Hit
GTATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGA640.12648221343873517No Hit
GTTTAATCCTGTGGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGG630.12450592885375494No Hit
GTACATGGGGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATG620.1225296442687747No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG610.12055335968379446No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG610.12055335968379446No Hit
GGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGGCCCTTGCTACTC590.11660079051383399No Hit
GTACAGATGCAAAGTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGA590.11660079051383399No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT590.11660079051383399No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG580.11462450592885376No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT580.11462450592885376No Hit
GAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAG570.11264822134387353No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC570.11264822134387353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11264822134387353No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA570.11264822134387353No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT570.11264822134387353No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA550.10869565217391304No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG550.10869565217391304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTT540.10671936758893281No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC540.10671936758893281No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT540.10671936758893281No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA530.10474308300395256No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT520.10276679841897234No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA510.1007905138339921No Hit
GTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTTGCTTTTTTTTTTT510.1007905138339921No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG510.1007905138339921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)