FastQCFastQC Report
Tue 10 May 2022
EGAF00003675934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53495
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1560.29161603888213855No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1510.2822693709692495No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1200.22432002990933733No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1160.2168426955790261No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1150.21497336199644826No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1140.21310402841387047No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1090.2037573605009814No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1090.2037573605009814No Hit
TGATAAAGTAGCATGAATGTGAAGCTCTTTGCTTCCTGGCCCCTAAAACA1080.2018880269184036No Hit
CCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCCCTGTCTCTAG1060.19814935975324796No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1050.19628002617067017No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC1020.1906720254229367No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1000.1869333582577811No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA990.18506402467520328No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC960.17945602392746984No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG960.17945602392746984No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG950.17758669034489202No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT940.17571735676231423No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG930.1738480231797364No Hit
GTACATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA890.16637068884942519No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT880.16450135526684737No Hit
CTATTGGCCAGGCTGATCTCGAACTCCCGACCTCAGGTGATCCACCTGCC820.1532853537713805No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT810.1514160201888027No Hit
GTATTAGCTCCATGACCCAGGCTGTTGACGTCTGCCCGGGCTGAAGTCAC810.1514160201888027No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG800.1495466866062249No Hit
GTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGAGAGGCTAAGG790.14767735302364707No Hit
GTTTAATCCTGTGGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGG790.14767735302364707No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA770.14393868585849146No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG750.14020001869333582No Hit
GGGTAAGGGTGGTTGCTGGTAACAGACAAACAAAGGGGCCCTTGCTACTC720.13459201794560238No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC720.13459201794560238No Hit
GTATCAACGCAGAGTACATGGGACGCACGGCCGGTACAGTGAAACTGCGA710.1327226843630246No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG700.13085335078044677No Hit
CCCATATACTCCATAGAATAACCCTGCCAAGGTGGGACTGTCCTGGCCCC690.12898401719786895No Hit
AGGTGGGACTGTCCTGGCCCCCTGTCTCTAGGTGAGGAGACTGAGGCAGA680.12711468361529116No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT670.12524535003271334No Hit
GTACATGGGTCGCCCAGCCTGGAGTGCAGTGGTGCACACAATCTCAGCTC660.12337601645013552No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC650.12150668286755771No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG650.12150668286755771No Hit
GTACAGATGCAAAGTAGTAGCTCAGAGGCTCTGGGTAATAGCATTCCTGA630.11776801570240208No Hit
GTACATGGGAAGCAGTGCCACCAGCCCAGATTTTTTTGCTTTTTTTTTTT630.11776801570240208No Hit
GTACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT620.11589868211982428No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT620.11589868211982428No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT610.11402934853724647No Hit
GTGTATACACTGCTTGCATAAACTCAACCAGCTGCCTTTTTAAAGGGAGC610.11402934853724647No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA600.11216001495466867No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC600.11216001495466867No Hit
GTACATGGGGAGGCCCTCTGCAAGAAGCTCAAGGAAGCTCAGGGAAAATG590.11029068137209085No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT580.10842134778951305No Hit
GAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGT580.10842134778951305No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT570.10655201420693523No Hit
GAGTATGGGGACGCCTGCACCAAGGCACACTCAGCACAGGAGCTGCAGGA570.10655201420693523No Hit
GCCTAGGATAGGACACGAGAGTCTGAAATTCAAAGCAACCAGCTTGAAGT560.10468268062435741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10468268062435741No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG560.10468268062435741No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT550.10281334704177959No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA540.1009440134592018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)