FastQCFastQC Report
Tue 10 May 2022
EGAF00003675952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003675952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186986
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA10010.5353341961430267No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT8010.4283743167937707No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG6190.33104082658594763No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC5640.30162685976490217No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT4720.2524253152642444No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA4500.2406597285358262No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT4470.23905533034558737No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA4280.22889414180740805No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG4170.22301134844319895No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC4070.21766335447573615No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG3710.19841057619287006No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT3700.19787577679612378No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG3290.17594900152952628No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC3110.16632261238809323No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA3100.16578781299134696No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG3000.16043981902388413No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC2950.15776582204015274No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC2910.1556266244531676No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC2840.15188302867594367No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT2750.14706983410522714No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT2750.14706983410522714No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC2740.14653503470848084No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT2730.14600023531173456No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG2720.14546543591498828No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA2670.1427914389312569No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG2630.14065224134427176No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC2590.13851304375728665No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG2580.13797824436054038No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC2570.13744344496379407No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG2570.13744344496379407No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT2550.13637384617030152No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC2470.13209545099633127No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC2440.1304910528060924No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG2430.12995625340934616No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG2430.12995625340934616No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA2410.1288866546158536No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT2380.12728225642561475No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG2370.12674745702886847No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA2330.12460825944188335No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA2250.1203298642679131No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT2250.1203298642679131No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG2210.11819066668092798No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA2210.11819066668092798No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT2100.1123078733167189No Hit
CTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTT2100.1123078733167189No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA1970.10535548115901726No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC1930.10321628357203214No Hit
GGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGAAAAAT1930.10321628357203214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)