FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT25.405405405405405No Hit
GCATAGCCATGTCCCTCTGCTCTACCTGATCCTGGCGATCAACTCGACGG25.405405405405405No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG25.405405405405405No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG12.7027027027027026No Hit
TTTCCGCATCCGGCCGGTGACCCCAGTGGAGAAGTACATCAAGGTCTTTC12.7027027027027026No Hit
GGCACTGTCCCCGGAGCGTGTCGCGGCCGGAGGCTCCCCGCCCGGGGGCC12.7027027027027026No Hit
GTCATGGGGAAGAGGTCGAACCCACGGATGCTGACGGCCGCGTACCCGTG12.7027027027027026No Hit
AGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG12.7027027027027026No Hit
CCCCTATTTTGCTACTTACTCTAATTATACTCAGCGCATTTGCCTTCTAC12.7027027027027026No Hit
GGTGAAACCCTGTCTCTACTAAAAATTAAAAAATTAGCCAGGCGTGGTGG12.7027027027027026No Hit
GTCGACGACATCCTCTAGTCAAGCAGGAAACTCGGTTCGAGAGACCATGA12.7027027027027026No Hit
CTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGCT12.7027027027027026No Hit
GTTTAATGAGTTTTCACTTATTGGATAAAAGTGTCAACATAGTGATGGCA12.7027027027027026No Hit
CTCATATCACGAACAGCAAAGCGAACCAAAGGTGGATAGTCTGAGAAGCT12.7027027027027026No Hit
GTTATAACCAGAGGCTCACAGGAATCTAACTCTGTATTGTTCCCCTAGGA12.7027027027027026No Hit
GGCACTGTCCCCGGAGCGGGTCGCGGCCGGGCGGCGGGCGGGCGGGGGCG12.7027027027027026No Hit
GTATTGGATGGCGTGGACCATGTTTTAGGTACCCATGTGATGACTAATAT12.7027027027027026No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT12.7027027027027026No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTAT12.7027027027027026No Hit
GAATAATAGAATGAACTCGAATGGAAACATCGAATGGAGTTGAATGGAAT12.7027027027027026No Hit
CCGTGAGGCTTCAGTAGCTGCTACCTGTCCAAACAAAATCTCAACTTGTC12.7027027027027026No Hit
GGACTTGTCGTCGATGTAGAGATTCTCGCCGGTCTCGAAAAACTTCTTCA12.7027027027027026No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA12.7027027027027026No Hit
GTGCTAGAGCACCACAAGAAGAAATTTGAACACAGAAAAATAAGGAAGCA12.7027027027027026No Hit
AGTCCCTCCTAGCCCTGGGCTCTGCTACCCTTTTCAGCATGGTCTCCTAC12.7027027027027026No Hit
GGTATCAACGCAGAGTACATGGGAGGAGGGTTTGTTCTCAGTGCACGTGA12.7027027027027026No Hit
CCATTGTTGAATTGGACACCCTGAAATGGCTGGCCAGTGACAGAGATGAA12.7027027027027026No Hit
CCCCGGATCACAGCTCATTTGCCAGCTCCCCGAGGCTTATCGCAGGCTAT12.7027027027027026No Hit
GTGGTATCAACGCAGTAGTTCCAGTAGCAGCTCCAGTACAAGTGGCAGCA12.7027027027027026No Hit
CCTTGGGAAATTGCTTCCTTGGAACGCAGCTAATGTGGATTGGCAGAAAT12.7027027027027026No Hit
GTCCTGCACTTCACCCACCACGGGCTCCTCGCCCAGCGGTGCGCCGAGGA12.7027027027027026No Hit
AGCTGAATAAGTAAGTGAATGCATGCGTGCATTATGGCCTGTCTGGCTGT12.7027027027027026No Hit
GCGAAGACGTGACAGTGCCGCGAGCGGAACTGCTGTCCGCGCTCATCTTC12.7027027027027026No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)