FastQCFastQC Report
Tue 10 May 2022
EGAF00003676113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930001
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT23070.2480642493932802No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20250.2177417013530093No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19450.20913956006498916No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC18630.20032236524476854No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18360.1974191425600618No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT16700.17956969938742No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC16570.17817185142811673No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT15440.16602132685878832No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14210.15279553462845738No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13710.1474191963234448No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA13290.14290307214723424No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG13220.14215038478453249No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA13070.14053748329302873No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG12890.1386020015032242No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12500.1344084576253144No Hit
ATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA12230.1315052349406076No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT11950.12849448548980055No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA11950.12849448548980055No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG11240.1208600850966827No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT11070.11903213007297841No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10970.11795686241197591No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG10680.11483858619506859No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT10650.11451600589676784No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG10570.11365579176796585No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG10350.11129020291376032No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA10130.10892461405955477No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT9820.10559128431044697No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT9770.10505365047994572No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT9760.10494612371384547No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9720.10451601664944446No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT9660.10387085605284296No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC9610.1033332222223417No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG9460.10172032073083792No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG9370.10075257983593568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)