FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC31.7341040462427744Illumina PCR Primer Index 1 (96% over 28bp)
GCCCTATTACGAGGGTTATAGTCGTGTGGCACCAGCGCGGCTACTCCGAT31.7341040462427744No Hit
AGATTAATATCCTAATAAAGGAAGCTTCTGAGAGCTTCCTGGCTCTTCCA31.7341040462427744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.1560693641618496No Hit
CATCTGACGCTGCCGACGAAAAAAAAAGTGTAGATCCTGTCTCTTATACA21.1560693641618496No Hit
GTGAGAGTGGGGGCTTATTCAAAGGAATTGTAAAAGATATGCCATAGTTC21.1560693641618496No Hit
GCATCCGACACGCGTTCTGAGCGTGCGACCTCGCCGTGCGAAGGAACGGG21.1560693641618496No Hit
TAATAAGAAAACTCCAAGTTAAGATACTGTTTTTTATCTATCGTATAGGC21.1560693641618496No Hit
ATTTTGAATGATCTGAAATTACAGATTGATAACAGACTGTCTCTTATACA21.1560693641618496No Hit
ACGCAGAGTACATGGGCATGCCTGTAATCCCAGCTACTCAGGAGGCGGAG21.1560693641618496No Hit
AGGTTGCGGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCAACAA21.1560693641618496No Hit
TTGCAACAATTGTAGATGCAGTGCGAGAAGGCAGAGGAATTTATGCCAAT21.1560693641618496No Hit
GGAGAGGCCGACCCGCACGGGCGGGAGCTCGGGATCGGCGCCGATGGTCT21.1560693641618496No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAA21.1560693641618496No Hit
AACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA21.1560693641618496No Hit
GCCACAGGCGTTGAAGCTGGTAATTCCTGGGCTGGTAAATACCATCATCG21.1560693641618496No Hit
GTACATGGGCATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTCGT21.1560693641618496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT21.1560693641618496No Hit
GATCCAAGTGTGGCCAATCACATCTGCTCTTGTAGAAATTTGTATTGGCT21.1560693641618496No Hit
GAACCAAGTTGGAAAACATACTTCAGGATATGATCCAGGAGCTGTCTCTT10.5780346820809248No Hit
CTTTCCTGAAAATAAACTACATTCCACAGTGATCTTTGGGGTTAGATTCT10.5780346820809248No Hit
ATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTCGTGTAGGGAAGT10.5780346820809248Illumina Single End PCR Primer 1 (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.5780346820809248No Hit
GGCTAACATGGTGAAACCCCGTCTCTACTAAAAAATAAAAAAAATAGCCA10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC10.5780346820809248No Hit
TGCCTGTAGTCGCAGCTACTCAGGCGGTTGAGGCAGGAGAATCACTTGGA10.5780346820809248No Hit
GACTTGACTTCTGCTGAGAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA10.5780346820809248Illumina Single End PCR Primer 1 (96% over 32bp)
AAAATTATCTGGGCATCGTGGAAGCCACCTGTAATCCCAGCTACTTGGGC10.5780346820809248No Hit
ATGACAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATATCCTT10.5780346820809248No Hit
GAACCTGGGAGACGGAGGTTGCAGTGAGCCGAGATCACGCCACTGCACTT10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAA10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAGA10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.5780346820809248No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGC10.5780346820809248No Hit
CCTCCAGGAATTACTGACCATAGTGCTCGTACGCTAGTCTAGCCTAGTAA10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.5780346820809248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.5780346820809248No Hit
GCCGTAATCACGCCTACACTTTTATCTACGTACTCCTTAAGTGTATCTTC10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAT10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATA10.5780346820809248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAACATATGACGGTGG10.5780346820809248No Hit
GTGTAGGGAAAAGTGTGTTCGGGCCGTCCCCATGTACTCTGCGTTGATAC10.5780346820809248No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.5780346820809248No Hit
CGTCAGATGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAT10.5780346820809248No Hit
TATCAACGCAGAGTACATGGGAAACCCTGTCTCTACTAAAAATACAAAAA10.5780346820809248No Hit
GTTGCTGTGTAGGAAGAGGGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.5780346820809248No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA10.5780346820809248No Hit
TCCCTGCTGTGGCATTGGAAGAGCCCAGTGAGTAGGAGGGAGAAGCAATA10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.5780346820809248No Hit
GTACATGGGACTCTCCTACACACAGCAACTCCAGTCCTTGCACATGAGAT10.5780346820809248No Hit
GGCGTCGCGGTAGTGCCGCTCTGCCGGCATCTCTGTCACGTCTGTCTCTT10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCT10.5780346820809248Illumina Single End PCR Primer 1 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.5780346820809248No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTC10.5780346820809248Illumina Single End PCR Primer 1 (95% over 24bp)
TCGTTGTAGAAGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAA10.5780346820809248No Hit
GTGGAGTGAAAGGCCCAAGTCCGCAGCCACGACTCTGTCTCTTATACACA10.5780346820809248No Hit
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTC10.5780346820809248Illumina Single End PCR Primer 1 (95% over 22bp)
GCGTCGATGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.5780346820809248No Hit
GCCAAATGACTCCTACTGGGTCCCTGGAGACCCTTTCTATATTATCACCT10.5780346820809248No Hit
GAGTAATGGAGGGACAAACAGCTTAAATTGTTAGCAACTACTAGACTATA10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.5780346820809248No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCACTGGT10.5780346820809248No Hit
TCTCTAAACAGGATGAGTCCGGTATGCCCATGTACTCTGCGTTGATACTG10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGGTAGCGGTGAAATGCGTAGAGATCTGGA10.5780346820809248No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCCTGTCTCTTATACAC10.5780346820809248No Hit
ATATATTGGCCGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGG10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGA10.5780346820809248No Hit
TGCCATGCCTTCGGCATCCTTCGCCTGCTGGCCGCGGTCCCACGCGTCGG10.5780346820809248No Hit
GTCATCGAATAAATTGAATGAAATCATCGAATGGTCTCGAATGGAATCAT10.5780346820809248No Hit
GAAGAAATGACTGCTTCTAAAGTCTGAGGAGTAAGAAACAAGATTGAAAA10.5780346820809248No Hit
ACGGTGGGTAGGTGGCAGGGTAGGACATACCCATCAGCATAGTCCAGTAG10.5780346820809248No Hit
TTCCCTCCTCCTTTCCAGGCAGTCCCTGGGGACCCCTCCTGCTCTGGTCC10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.5780346820809248No Hit
GTACATGGGAGATCTGTTGAAGCAAATCTTGTTTGGCACTGAGCTATTCA10.5780346820809248No Hit
ATAAAGTATGAGAGGAAATGATATATGGCACTTTAGAATAAAAGCTTTAG10.5780346820809248No Hit
ATCAACGCAGAGTACATGGGAAGCAGAGGTACTGTCTCTTATACACATCT10.5780346820809248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA10.5780346820809248No Hit
AGGCTGGAGTGCAGTGGCATAATCTTAGGTCACTGCAACCTCTGCCTCCC10.5780346820809248No Hit
AGCCTGGCTTGCCGAACCCTGGATCTTGTTCCTGGTGACCCAGGGACACT10.5780346820809248No Hit
TTTGAATACATCCAGCTTTCCATCTCAGATCTAGTTTCTGTCTCTTATAC10.5780346820809248No Hit
GCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.5780346820809248No Hit
ACAATCCATGATGTACCTGAAATATGTGAAGAAGTAAATGAATATGTGTA10.5780346820809248No Hit
CTACAGCATCATGCACTTCAGTTGTTTTTTTTCCTCATAGTCTCCTGGTT10.5780346820809248No Hit
CCCTGTGTGTGACATGGTGGAGGGTCTGTCCCTGAGCCGGCAGCCTGTAC10.5780346820809248No Hit
CTTTTACACCGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCCA10.5780346820809248No Hit
TCAACGCAGAGTACATGGGGCATACCGGACTCATCCTGTTAAGAGATCGG10.5780346820809248No Hit
AAAAAGTACTCTGCGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT10.5780346820809248No Hit
ACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG10.5780346820809248Illumina Single End PCR Primer 1 (100% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5780346820809248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTATAAAA10.5780346820809248No Hit
CACATATTGAGAAGGAACTCCAAGCAGACATCTGTGCTCTCCAATGACCT10.5780346820809248No Hit
GCAGACCCCTGCGCCATAGCAGCTTTAGAGTGGCCCAGCCCGGGGCTTAG10.5780346820809248No Hit
GATGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATCTGCGTTGA10.5780346820809248No Hit
GCAAGATCTCGTATGCCGTCTTCTGCTTGCCCATGTACCTGTCTCTTATA10.5780346820809248Illumina PCR Primer Index 9 (96% over 27bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTA10.5780346820809248No Hit
CTCCAATAGCATGCTTATCTTCCTCTTTCTTGCTGTCTTTCTTACTGGGA10.5780346820809248No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT10.5780346820809248No Hit
ACACACCACCCTGGACAACTATCCCGGCCGCGTTGGTCAGCTCGATGGCA10.5780346820809248No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGCAG10.5780346820809248No Hit
GGTATGCTTTGTCTAATATCTGTGGAACACATGGAGTGACATAACCTGCC10.5780346820809248No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACGCTGCGTTGTTA10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTGTTACC10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGAGTG10.5780346820809248No Hit
GGTTCAAGTGATCTTCCTGCCTCAGCCCACCAGTAGCTGGGATTACAGGC10.5780346820809248No Hit
TATCAACGCAGAGTACATGGGATATGAGATCTACACTCTTCCCCAGCAAC10.5780346820809248No Hit
GGTATCAACGCAGAGTACATGGGTACGGCGACCACCCAGAAAAAAAAAAA10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA10.5780346820809248No Hit
GCTAACTCAGTTCTGTCTTTCCCCATCCTCTCTCTGTTTTAAAAAGAAAC10.5780346820809248No Hit
ATCTGGTACACCTGGAGTAGGAGATGAAAATCTTCTTTCTTGCTGTCTCT10.5780346820809248No Hit
GATGTGGGAGGATCACTAGAGCCCAGGAGGTGGAGGATGCAGTGAGCCAA10.5780346820809248No Hit
GTACATGGGGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGGCTGGGCGTGATGGCACATGCCTGTAGT10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA10.5780346820809248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTTAACCACTGCCCCATG10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.5780346820809248No Hit
CCAGTAATGGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATCCCTG10.5780346820809248No Hit
TCTATAAACCTGGAAGAAAGCGCTCACCAGAAATTGATCTTGGACTTCCC10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAC10.5780346820809248No Hit
GATCTCGGTGGTCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC10.5780346820809248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.5780346820809248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAAAAC10.5780346820809248No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGGGGGTTT10.5780346820809248No Hit
GCAGAAGACGGCATACGAGTTTTTTTTTTGTCTTGTGGGCTCGGAGATGC10.5780346820809248RNA PCR Primer, Index 19 (95% over 24bp)
GATACGGCGACCACCCCCATATACACATTTTTTTTAGATTTCTCTTAAAA10.5780346820809248No Hit
GCTCTGTTGTGTACCATGAGGTTCTCAATGCCATGGAGTGCAAATGCTCC10.5780346820809248No Hit
GCCTACACTTCCTACACGACGCTCTTCCGATCTCATGATGCACTGTCTCT10.5780346820809248Illumina Single End PCR Primer 1 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5780346820809248No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10.5780346820809248No Hit
GTACATGGGTGACCAACCTGGCCAACGTGTTCTCTACTAAAAATACAAAA10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAGGAAAA10.5780346820809248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATA10.5780346820809248No Hit
TATTGGTCAGGCTGGTCTTGAACTCCTGACCTGAGGCTGTCTCTTATACA10.5780346820809248No Hit
GTCCAAAGCGTGGACAACGGTTCAAATTGCACGTCATCCTGAACGTCCGC10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGGT10.5780346820809248No Hit
GAGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTGT10.5780346820809248No Hit
GTGTGTGTGCCGGCATCTGTGTGTGGGCATCTGTGTGTGCCGGCATCTGT10.5780346820809248No Hit
ATCTGACGCTGCCGACGAAAAAAAAAGTGTAGATCTCGGTGCCCATGTAC10.5780346820809248No Hit
GCTTATCCACTTCTTTTTGAGAGCCAACCGTATAAAGAGTTGTGCCTGCG10.5780346820809248No Hit
GTACTTGAGATCAATCACTCAACCTACATTATGGCTGGGCACAGTGGCTC10.5780346820809248No Hit
ATTTTACTACTGTGTGAATTAGAAATCAAATTCCCAATGGTTTCAATCCT10.5780346820809248No Hit
TACAAGAATTAAATAACATGTCCAATGCCTTACCTGTCTCTTATACACAT10.5780346820809248No Hit
ACGACATGGAAGCCAGACCAACTTAAGTGGGTCTGTCTCTTATACACATC10.5780346820809248No Hit
GCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTT10.5780346820809248No Hit
GTACATGGGAAGCGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5780346820809248No Hit
CGTCAGATGTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACGAG10.5780346820809248No Hit
TCGTTGTAGAAGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA10.5780346820809248No Hit
ATCTAGAGTGTTGAACTAAATTGTCTCCTGTACATCTGGCTATGAGAGAA10.5780346820809248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)