FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGCTCTTCGGTTTGATAAGGGTAGGTCACCAAGATCCATATTTCCT23.4482758620689653No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA23.4482758620689653No Hit
CCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGT23.4482758620689653No Hit
GAATCATCATCGAATGGAATCAAATGGAATTAATGAATGGAATTGAAAGG23.4482758620689653No Hit
CTCAGCGCGTCTTCGAGCAACCGGTCGAAGTTGGTGGTCACGATGACGCG23.4482758620689653No Hit
GTAAGAAACATCACCCATCTAATGCTAGCTGTATGAAACACACACGCACA23.4482758620689653No Hit
AGTTCCAAATATACTAATCTGACCATTTGCTCATTACACTCCAGTCACAT23.4482758620689653No Hit
GGTTAGAAACCTTTAGCCTCGGCAGGGCGCGGTGGCTCACGCCTGTAATC23.4482758620689653No Hit
GCGCCATGGTGACGATCTGTTCCGGCGTCTTTCGGCCGGCGGCGATGACC11.7241379310344827No Hit
CCTGTGCATGGCTCGTGTCTCTCATCATACGGGGTCCTGTCTCTTATACA11.7241379310344827No Hit
GAAATGCATGGCTTCACCAAGAACTTAAATGAGGCAGTGCCTCAATCTAG11.7241379310344827No Hit
GTAGGGGACACAACAGCAAGTGGGGTCATTGCCTTTACCATACTGTGTTG11.7241379310344827No Hit
GTGGAGTACCAATCTGTTTTACAGCCAGGCTATACTGTTTTAACTATTGA11.7241379310344827No Hit
CTTTTCAACTTAACTTGTTTCCTAATGAGATTAAATTCATGCATAAATAA11.7241379310344827No Hit
CGGAAGAGGGCGTGCAGGCGCTCGTTGCCGCCGAAGTCCGGCATCTCGCG11.7241379310344827No Hit
AGTTGCTACACCTTGGGCATTAGCTACAGCTTCACCGATCACGACACCAT11.7241379310344827No Hit
AGTTTAGGCATGCCGTCTTTCAAACCAGAGTTTTGAACAGAATTTGAACA11.7241379310344827No Hit
ACATCAACATCTCTGGGATACAGCAAAAGCAGTGTTAAGAGGAAAGTTTA11.7241379310344827No Hit
CTTCGACACGTTGGCGTTCCCCGATGCGCTGCGCATCCGGGACATCAACG11.7241379310344827No Hit
CCGTCGAGGATGGCCCGGGTGACCCCGGCGACATCGCCGGACCAACGCCA11.7241379310344827No Hit
GCCGCCGCCCAATCCAGGGTCTCGGCCACACCAGGCACCTTGCGCAATTC11.7241379310344827No Hit
TATGAATGAACCAAACAACATAATAAAAAGACAGAGATTGTCAAAATGGA11.7241379310344827No Hit
ATGGAATGGAATGTACATGAAAGTAATGAACTGGAATGGAATGGAGTTGA11.7241379310344827No Hit
GGTTCTAGGACAGCATCTCCCCTGGTGCTGGAGGTGCTCAAACCACCATG11.7241379310344827No Hit
GCGTGATCGCCTCGTTCATCGGCACCACGGGCGCCGCGATGCTGCTGATC11.7241379310344827No Hit
GTCCCAGGTCCCTCCCAGTCTAGCATTCCTTTGCAGGTTTGCTCTGCCCC11.7241379310344827No Hit
GACCGGGCCGGTCATGGTCCGCTGGAACATCAGCAGGATGTAGACGGCGG11.7241379310344827No Hit
CTCATCCGCCTGCGCGAGGGCCAGACCGTGCGCCTCGCGGTGACCAACCA11.7241379310344827No Hit
GGCCCCGACAGCGTGCCGCTACGATTCGGTCCCATGCGGAGCTTTTTCGA11.7241379310344827No Hit
AGGCCCACCTGCTCACCGAGTTCGACGAGGGCTGCGATGACCTTGTCGTC11.7241379310344827No Hit
CCACCCGACCGGGGCACGCGCGTCACGCTGCGTCATGCATGGGCGTCGGG11.7241379310344827No Hit
GGGCTTGCTTCCCAGGCTTGTATGCTGGTTGCCATAAAATGTGTCAGACA11.7241379310344827No Hit
CTGACAGCAAGTTTTATATGGTTCTTTCTCAAGCTTGACCTCTACATTGT11.7241379310344827No Hit
GTCCTTGAGCCTTATTTCAATGATCTTTACGATCACTCCATGCGGGTGTC11.7241379310344827No Hit
AGATCACATCCGAGATCATCACCGAAAAATTCGACGACACCACCATGATC11.7241379310344827No Hit
ATCTAATGGAATCATTGAACAGAATTGAATGGAATCGTCATCGAATGAAT11.7241379310344827No Hit
AAGCTATCCAAATATCCACCTGCAGATTCTACAAAAGGAGTGTTTCCAAA11.7241379310344827No Hit
AATCCCAGCACTTTGGGAGGCAGAGGTGGGAGAATCACCTGAGGTCAGGA11.7241379310344827No Hit
GCATAGCTGAGGGTGGTGTGGCCGGGCGGGACACAGGGTGTTTCCTCTGC11.7241379310344827No Hit
CTTCAGGAGGATTATTCTGAATTCTTTGTTATTTTGGAGAGTTTCATTAC11.7241379310344827No Hit
GCATCGGCCTGCACGAGTACCGCCGGCGCCACCGCGCCGCCTTTGATACC11.7241379310344827No Hit
GTTTCGGCCATCTAGATAGTCGGCATTGAATGAGAGAGCGACCTGTCCAT11.7241379310344827No Hit
CGGCGCAACGCCAAATCGCGCACGCGCCCCATCGGCCGCGGCTGCGGGTG11.7241379310344827No Hit
ATTCCATTCCCCTGCAGTCGGGTTGTTTCCATTCCATTCCATTCCGTTCC11.7241379310344827No Hit
CCGGCGACAAGTGGAACAAGTACATCTTCCGCACCGGCCGCAATTCGAGC11.7241379310344827No Hit
GCCTCAGCCTGGCCCAGTTGATCTCTTAAACTTTCTTCCAGCTCTGAATC11.7241379310344827No Hit
AACACGAGCAGACAAACTTTTCATTTCTCATATCCCTAGCAATGATGCCT11.7241379310344827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GCATGTCAATGACTCCTAAATGCATGTTCTAAACTCAGGACCTGTCAATG11.7241379310344827No Hit
GTCCTGGATCGAACGGCGAGCGCTCTTCAGGAACAGGTCGGTACCTTCAC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)