FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCAGATCACCTG23.4482758620689653No Hit
GCCTTAGCCTCCCAAGTAGCTGGGATCACAGGTGCCCGCCACCACGCCCA23.4482758620689653No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT23.4482758620689653No Hit
GTGTGAGAGCATGCAGTCAAGATTTTATTTAGACTGAGTAGATTTGGGAC23.4482758620689653No Hit
GCGGGCGGCAAACCGATTGCTCCGGAGCAGGACTGGGAGACGTGGTGGCG23.4482758620689653No Hit
CCCTGTGAGGGAGGAAAGAAGGATCATTTGCCCCGCTGGGTCTCAAAGGC23.4482758620689653No Hit
CCATTTGATTCCATTTGATGATTCAGTTCGATTCCTTACAATGATTCCCT23.4482758620689653No Hit
TCTCTGGGATTCCTTCTCAGGTGAGATTATCTGGACAAATCACACCTGCA23.4482758620689653No Hit
CTGCTGCAGCTGCTGCTCGATCTGCTGCGCGTCGCGCGCGATCATCTCCT11.7241379310344827No Hit
CCAAATAGCTGAGATTACAGGTGCGTGCCACCACACTTGGCTAATTTCTG11.7241379310344827No Hit
GTCCAAGAACCTGGTGGACGACAAGGTCATCGCAGCCCTCGTCGAACTCG11.7241379310344827No Hit
CACGATCACCGATCGCCAGGGCAACGCCCTGTCGTGGGCTTCGTCGGGTG11.7241379310344827No Hit
ACCGGCAGATTCTCCACGCCCGAGATCCTCGCCTGCAGCGCCGCCAGCCG11.7241379310344827No Hit
CTCATCGGCCAGGCCAAGGGCATCCTCATGGAGCGGTTCCGGCTCACCGC11.7241379310344827No Hit
GGTTTAGGATTGAGAAACTTGGATGTCATTATTTAGTCAACAGAATTTGG11.7241379310344827No Hit
GCTTCATGCCCATACCCCGGGAGAGTTCCTTGACCTTCTTGGTGTCCTGC11.7241379310344827No Hit
GCATTCGAGTCCATGGATTATTCCATTCCATTCCATTAGATGATTCCATT11.7241379310344827No Hit
AACTTCTTTGTGATGTTTGCATTCAAGTCACAGAATTGAACATTCCCTTT11.7241379310344827No Hit
GAGTGGAATGGAATGGAATGGAATGGAATGGAATGGAATCAACTCGATTG11.7241379310344827No Hit
CTGCCAAGGTGATGTGTGCAGCCTGGGTACTGCAAGGCCTTGTGTCCTCC11.7241379310344827No Hit
GGAGAACTTCGCCCGGCTGGAGGAGATCGGGCGCGAGGCCGGCCCAGGCT11.7241379310344827No Hit
TGCATGACGTAACTGAGTGCTTGGCAATCGTCGGCTTTAACCGATTAAGC11.7241379310344827No Hit
TGCCTGCCCAGGGTTATTACCAACTGGCCCAACTAGAATTGTAGGCTAAA11.7241379310344827No Hit
CCAAATCCCCAAGCTCTGAATGCCTGGCTGTTCCTCTCAGCCTTTCTTTG11.7241379310344827No Hit
GTGAGGGCAGAAGCTGGGGCAGTAGCCACATTGGCATCTCACCATGAGTC11.7241379310344827No Hit
TACACACACACACACACACGTAGAAAAATACAGACAGAGGAAGAACTTAC11.7241379310344827No Hit
GACCTACCTCGAGCGGCACACGCTGAAGGCCATCACAGGCTTGGCCGAAG11.7241379310344827No Hit
GCACCAGCAGGTTCAACTGTTAAGCTTTACAATACAGATGGTGTCGTGAT11.7241379310344827No Hit
TCGAAAGCATCCGCAAGGAGGAATTGCGCAAGGTGCCTGGTGTGGCCGAG11.7241379310344827No Hit
GTTCCTCTCTGATTTTAGTTATTTCTTCTCTTCTGCTAACTTTGGAGTTA11.7241379310344827No Hit
GCCACAACTCTCTAGGTGACAAAGTAGACAATGAGATAAGGACAGTTTAT11.7241379310344827No Hit
ACCTACGCCTTTTAGAGGTAAGGAAAGCAAGCAGGCACAGAACACAAGAC11.7241379310344827No Hit
GACCCCGTATGATGAGAGACACGAGCCATGCACAGGCTGTCTCTTATACA11.7241379310344827No Hit
ATACAGGAGGACACTCGACGCAACGACCAGCACCGCAGAGATCCATAGCC11.7241379310344827No Hit
GGCTGGTCTTGAACTCCAGACCTCAGGTGATCTGCCCGCCTCGGCCTCCC11.7241379310344827No Hit
GATCGCCGACGGCCTCAGTGCCACCGCGGTCCTGACCACAGCCACCGACT11.7241379310344827No Hit
GGTTCCCACTCTGCTTGCGTAGTTGTAAGAAGGACAACCACCACTGATCA11.7241379310344827No Hit
GGCAAGGGCCCTCTAATGCATGGACAGGTCGCTCTCTCATTCAATGCCGA11.7241379310344827No Hit
CTTTATAGCAGCATGATTTATAGTCCTTTGGGTATATACCCAGTAATGGG11.7241379310344827No Hit
AGCGCGCGGTGTTCGACGCCGCCCCGGACGGCATCGCCGTCGTCGACGAG11.7241379310344827No Hit
CGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAA11.7241379310344827No Hit
GAGCCATCGCGACCCTTGGCACGGTGCTGGCCGCCGTCTACATCCTGCTG11.7241379310344827No Hit
AGAATGAACATATACATCAAGATCACAGTCCTTTGAGAGCTTAGAGTATG11.7241379310344827No Hit
GGTCCAATGTCGGCAGCGATTTCCACCTTTACGCCTTCAACGACCTCGGC11.7241379310344827No Hit
CTCAAGAAGGCCAAGCTGGTCCACGAGGAATACCTGCCGCCCGCCACCAC11.7241379310344827No Hit
CCAGTCCATTCCTTTCGAGTCCATGCCATTCCAGTCCACTCCTTTCCATT11.7241379310344827No Hit
CAGCCTGACAGTGCTGCTTTCAACAGCAGAACAGCTATCTCCCAAATCCC11.7241379310344827No Hit
TGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
AGACGACGGTGTGCGCGCGGAACTTGCGCTGGCGGTTCGTGCGCAGCAAC11.7241379310344827No Hit
GCTGACCCCGGGCACACCGTCCATCTGCATCGGCACGAGCGCTCCGTGCC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)