FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTAATCTGTGACATCAATATCATAAAGAAGTGAGCAGAGCTTTACAG34.054054054054054No Hit
GGTGAAATCTTATCTCAACAAATACTTATGTATGCGCTCACGACAAATAT34.054054054054054No Hit
GCGTCCTCCTCGAGCACGGTGTTGCGTGCCTTCAGTGCCGCCAGCTCCTC22.7027027027027026No Hit
TTTCCAAGTCAGTTCAAATAAAGACATCCTTGCAAGTGGAGTCTCCCAGG22.7027027027027026No Hit
GACACACACCACCATCCCCTGCTAATTTTTTGTATTTTTTGTAGAGACAG22.7027027027027026No Hit
GATCGGCTGCACCAGCTGGGCGCAGGTGCTGCTGAAGTTCGTGCTGAGCC22.7027027027027026No Hit
CCCTTCTCCCTTCCTTTCTTTTTTTCTGCTGGAATGCAGATGTGATGGCT22.7027027027027026No Hit
AGTGGAAGCATTGAGTGGGCTCAAAAGAAATCATCGGAGTATGGAATTGA22.7027027027027026No Hit
GATAATTCCATTCGATTCTATGCAATGATTCCATTCCTTTCCATTAGAAG22.7027027027027026No Hit
GCGATACAGTGCGAGGCGGCGCGACAGCGCCTCCGCCTGATCCGCCTCCG22.7027027027027026No Hit
ATCAGGTGCTGGGCGGCACGATCAACGCGACCGGGGCCCTCATCGTCGAG22.7027027027027026No Hit
CCGAGAACCGTGCGCGCGGCGTGGCCAACTACCTGAGCCAGGTGGTGATC22.7027027027027026No Hit
TCTCGGTGGCTACAGCGTCTTGCTCAGAACGCCGCCGGTTTCCGTGAAGC22.7027027027027026No Hit
CCTTTCAGGGTCTCTGGTCTAAACTGGGAATCTCAGAGTCAGCTTATCCT22.7027027027027026No Hit
GTGATCATCTCACCTCAGCCTCCTAAGTAGCTGGGACTACAGGTGCACGC11.3513513513513513No Hit
GTCCCGTGACGTCGCGCGATGACCTGCATCTGGGCGGGAACGTCATCGGT11.3513513513513513No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGT11.3513513513513513Illumina Single End PCR Primer 1 (97% over 38bp)
TTTCAATACTGCTCCATAAAAAGAAAGGTTCAGCTCTGTGTGAGGAATGC11.3513513513513513No Hit
TCGCCCGAGTGGTTCGAGGAGACCCCCACGCGCGTGCGCGCCGTGCCCAT11.3513513513513513No Hit
CAACCTTTCCTAGTGCTTAGGCTGCCGCGCCCGAAAGGCGCGGCAGGATG11.3513513513513513No Hit
TACCTAATGCCTCCTCCTCCCTTGCCCCCAGCTAACTAGTAGAAGAAGGG11.3513513513513513No Hit
GCTCTCAGTCAGCTATGTGATCATCGGGGTAAACCATCAATACTCTATAG11.3513513513513513No Hit
GTTTGTAGATTCTGGATATTAGCCCTTTGTCAGATGAGTAGATTGCAAAA11.3513513513513513No Hit
TGTTCGCGCAGGCGGGGTTCACCGTGCTGCGCTTCACGTGGGAGGACCTC11.3513513513513513No Hit
GAAGGTTAGAGAGTGTGAGTCATTTGTACAAGGTCACTAGCCTGGTTGCA11.3513513513513513No Hit
ATCACCTTGTACCTGAGGAAAGAGGCCCAGAGATAGGAAGGACACATGGC11.3513513513513513No Hit
TCATTATCGAATGGAATTGAATGAAATCATAATCGAATGGAGTCGAATGG11.3513513513513513No Hit
GCTTATACACTGCTGGTGGAAATGTAAATCAGTTCAACCACTGTGATTTC11.3513513513513513No Hit
TTTCAAAACTGCTCCATAAAAAGAAAGGTTCAGCTCTGTGTGAGGAATGC11.3513513513513513No Hit
CATTTCCGGCCGAGCCGAGCGACTGACGTTGATGGCCCAGGACCCTGATG11.3513513513513513No Hit
GTGCAGGACGTGCCTTGTTAACAAAATGTTTACAAGCAGTATACTTGGTA11.3513513513513513No Hit
CTTCCCTTCCTCTGCAGAAAACATAGATGCTGAAATTCCAAATGCCTCCC11.3513513513513513No Hit
ATGATAAAACATCAAACATTAAATGAGAAGAATTATTCTGGTCCTTTATT11.3513513513513513No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.3513513513513513No Hit
GTTGGGAGTTTGTTTGAGCAGGGATTTACCTGTTGAACCACATAGAGAAT11.3513513513513513No Hit
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA11.3513513513513513No Hit
CTGGCCGAGCGCGAGGATGACGGTGTCGGCGGCGAGTTTCTCGTACCGGC11.3513513513513513No Hit
GGATGGCAGGCATGAGTGACTGCACCCAACCAACTCTTTCTTTTAATACC11.3513513513513513No Hit
AGGCGGTGTTGAGGCTGACCTCGGTGGCGATCTCGTGCGGGGTGTCCTTG11.3513513513513513No Hit
CCGTCAGTCAGCGTGACGCTATCCAGCTTCTTGGAGCTGTTGATGATGCT11.3513513513513513No Hit
GGTCTGGAGGACAGTGGCCTTCTTCTCACAGCTCCACTGTGCAGTGCCCC11.3513513513513513No Hit
GCCTCACCCAGCACCTCCCCTTCTGACAGACACCTCAGCCCACAAGGGGC11.3513513513513513No Hit
GGTGAGTAGCATTTCAGCAGAATAACAGCTGTGCCTGTCAAAAGTGACAT11.3513513513513513No Hit
CCATATAGTTGCGTGGGTCGAAGTCGCCGAGGACACGCGCCCGCTTGAGC11.3513513513513513No Hit
GACAAAGTGCTTCTAGGTGCTGGGTGTCTGACTTTTGGCTTTCAGCCTGT11.3513513513513513No Hit
GACACCACCACACCGAAGCCCAGAACGGGCTTGGGTCCGAACTTGTCGGT11.3513513513513513No Hit
CTTTCAAGGCCAGTCAGAAAAGATCAGACCAAGAGTTCATCAATGCAAGA11.3513513513513513No Hit
GTACGAGACCGGCGGCCGTCTCCCGGCGCTGACCGAGTCCGCCGACGCCG11.3513513513513513No Hit
CGCTGACGGTTACCCTTGGACTCGTGCCCAACGACGACCTCGCACTGCTG11.3513513513513513No Hit
ATACTCAGGCATCCAAAGACAATTTATAACTCTATGAATTGTGCTGAGCT11.3513513513513513No Hit
ACTGTAAGCGATATAAAGGGGTCTGAGAAGGCCACCGCAGTCATTTCTTC11.3513513513513513No Hit
ATGTACATCTACCGCGGCATCAACGTCTACTGGGCGGGCAGCAGCCGAAT11.3513513513513513No Hit
CAATAGAGCAAGGTGCCATCTCAAAAAAAAAGAACAAAAATTGGCCGGGC11.3513513513513513No Hit
CGATCATGGCAGAAGGGGAAGCAAACACATCCTTCTTCACATGGCAGCAG11.3513513513513513No Hit
TAGTCATAGTGTACATAAAAACAGATTTGATTTTCTTGGAATCAAACATA11.3513513513513513No Hit
GATAAGAAATGCACATAATGATCTCTTCCAAGCTGGTATAACTAACTCTA11.3513513513513513No Hit
ATCGGTGCCTATGTGACGCTCCGCGGCGATCGCATGTGGGAGTTCCTCGA11.3513513513513513No Hit
TTTCAAACATGCTCTATGAAAGGGAATCTTCAACTCTATGAGTTGAATGC11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)