FastQCFastQC Report
Tue 10 May 2022
EGAF00003676423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320761
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11180.3485461137731832No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT10570.329528839229208No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9900.3086410130907436No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG9840.30677046149625425No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9600.29928825511829676No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8080.2519009480578998No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7430.23163663911759846No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7020.21885453655525453No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6510.202954848002095No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA6180.19266681423240356No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG6090.18986098684066954No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6070.18923746964250643No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG5850.1823787804627121No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC5790.18050822886822276No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5730.17863767727373342No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5600.17458481548567314No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5390.1680378849049604No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC5070.1580616097343505No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC4800.14964412755914838No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT4690.14621478296925125No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG4680.1459030243701697No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC4390.13686202499680447No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG4220.13156212881241797No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC4050.12626223262803146No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC4020.1253269568307868No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4020.1253269568307868No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA3910.12189761224088963No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC3780.11784475045282937No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT3750.1169094746555847No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG3650.1137918886647691No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3610.11254485426844286No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA3600.1122330956693613No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3580.11160957847119819No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC3570.11129781987211662No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC3550.11067430267395349No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3530.11005078547579039No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA3430.10693319948497479No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC3350.10443913069232232No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG3340.10412737209324076No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3320.10350385489507764No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3260.10163330330058828No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG3250.10132154470150674No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG3210.10007451030518048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)