FastQCFastQC Report
Tue 10 May 2022
EGAF00003676436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450334
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC20130.4470015588429921No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18440.4094738571815586No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG18060.40103567574289306No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT18000.399703331305209No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC15970.3546256778302327No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA15780.3504065871108999No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG13690.3039965891982395No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT13600.3019980725417135No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT13130.2915613744465219No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12660.2811246763513303No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC12450.27646147081943623No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12240.27179826528754214No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG11850.26313802644259593No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG11450.25425573019136904No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11020.2447072617213002No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG10680.23715730990775735No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG10650.23649113768891536No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG9920.2202809470304263No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9660.21450745446712885No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA9470.21028836374779608No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT9250.2054031008096213No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA9210.20451487118449863No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9170.20362664155937593No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG8890.19740903418351713No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC8600.19096936940137765No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA7400.16432248064769706No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC7230.16054750474092563No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG6830.15166520848969875No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC6820.15144315108341808No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA6720.14922257702061137No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT6590.14633583073896264No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT6500.1443373140824366No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC6480.14389319926987526No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC6220.13811970670657778No Hit
GTGGTAAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAG6210.13789764930029713No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG6000.13323444376840302No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT5930.1316800419244383No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA5800.12879329564278957No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA5770.12812712342394755No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC5700.12657272157998287No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5690.12635066417370217No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC5580.1239080327046148No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC5550.12324186048577278No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC5540.12301980307949212No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC5470.1214654012355274No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG5450.12102128642296607No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA5440.12079922901668538No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC5290.11746836792247532No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC5290.11746836792247532No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA5280.11724631051619465No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT5220.11591396607851061No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA5220.11591396607851061No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5180.11502573645338793No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT5120.1136933920157039No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG5100.11324927720314254No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT5050.11213899017173919No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC5040.11191693276545853No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC5020.1114728179528972No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG4990.11080664573405517No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG4970.11036253092149381No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG4960.11014047351521315No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC4960.11014047351521315No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG4830.10725372723356442No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4820.10703166982728375No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC4810.10680961242100309No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT4800.1065875550147224No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA4760.10569932538959971No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC4710.10458903835819636No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG4690.10414492354563502No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC4640.10303463651423167No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA4620.10259052170167031No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC4560.1012581772639863No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA4560.1012581772639863No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG4510.10014789023258294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)