FastQCFastQC Report
Tue 10 May 2022
EGAF00003676448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438252
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC9160.20901216651606838No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC6840.15607458722378906No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG6830.15584640800270164No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6690.15265189890747788No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA6420.14649105993811778No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5940.13553845732592207No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT5810.13257212745178573No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC5560.12686764692460045No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC5560.12686764692460045No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5520.12595493004025082No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC5360.12230406250285224No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC5140.1172841196389292No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5090.11614322353349214No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5020.11454596898588026No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC4980.11363325210153064No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC4970.11340507288044321No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG4970.11340507288044321No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4780.1090696676797824No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC4740.10815695079543276No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT4740.10815695079543276No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT4580.10450608325803419No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT4520.10313700793150972No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG4450.10153975338389785No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC4430.10108339494172303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)