FastQCFastQC Report
Tue 10 May 2022
EGAF00003676466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264128
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT106050.8389182108140948No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC78620.621930690562981No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC77390.6122006632239773No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT68110.5387903756581612No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA61430.4859476255569056No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG60260.47669223369785335No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA58190.46031730963953016No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT55900.44220205548805186No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA51660.4086611482381531No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG51270.40557601761846906No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG48980.3874607634669907No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT48620.3846129505872823No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG47280.3740127582017011No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT47050.3721933221952207No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA44400.35123025516403406No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG43800.34648390036452004No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC43710.3457719471445929No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT41980.33208662413932766No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC39880.31547438234102876No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT39660.31373405224787365No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG39540.3127847812879708No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG39000.3085130619684082No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT38930.30795932057513165No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC38560.305032401782098No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC36330.28739178311057106No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG35310.2793229799513973No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG35240.2787692385581207No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA35180.2782946030781693No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT33060.26152414945321995No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC32890.260179348926691No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC32420.256461371000405No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA32360.25598673552045365No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC32150.25432551134062376No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA31490.24910452106115835No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG30550.24166856520858648No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC29950.2369222104090725No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC29910.23660578675577157No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG28130.2225249341838801No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG27580.2181741089509923No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA27390.21667109659781286No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC27220.21532629607128392No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT27030.2138232837181045No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC26950.21319043641150262No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC26640.21073815309842042No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG26030.20591269238558121No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA25770.20385593863912516No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT25750.2036977268124747No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT25550.20211560854597002No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG25270.19990064297286353No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC24540.1941259113001215No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG24370.19278111077359253No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC24230.1916736279870393No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT24150.19104078068043742No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC23800.18827207371405427No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA23370.18487051944106925No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG23100.18273465978128797No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT22800.180361482381531No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG22720.17972863507492914No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC22300.17640618671526934No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG21930.17347926792223572No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA21690.17158072600243013No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT21600.17086877278250304No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT21360.16897023086269744No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG20620.16311639327663022No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG20590.1628790755366545No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA20560.1626417577966788No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG20400.16137606318347508No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG20290.16050589813689753No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT19680.15568043742405832No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA19570.15481027237748077No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA19530.15449384872417984No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT19500.1542565309842041No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG19400.1534654718509518No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA19390.15338636593762658No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT19310.1527535186310247No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG19130.1513296121911705No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT19110.15117140036452004No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT18740.14824448157148643No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA18480.14618772782503037No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT18430.14579219825840423No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT18160.14365633859862292No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC18120.143339914945322No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC18020.14254885581206966No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT17910.1416786907654921No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG17680.13985925475901176No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC17430.13788160692588092No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA16960.13416362899959497No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA16950.13408452308626975No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA16810.1329770402997165No Hit
TGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGC16790.13281882847306603No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT16740.13242329890643986No Hit
TGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTC16710.13218598116646416No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG16550.13092028655326043No Hit
GCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCT16520.13068296881328473No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT16500.13052475698663427No Hit
GCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTT16230.12838889732685296No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG16140.1276769441069259No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG15850.12538287262049413No Hit
GCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCAC15480.12245595382746051No Hit
CTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAG15440.12213953017415959No Hit
CCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGG15350.12142757695423248No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT15290.12095294147428108No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT15220.12039920008100446No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG15200.120240988254354No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC15190.12016188234102876No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC14920.11802602268124747No Hit
CCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCC14830.11731406946132038No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT14570.1152573157148643No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG14500.11470357432158769No Hit
GACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCA14470.114466256581612No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC14440.1142289388416363No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA14270.11288413831510734No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC14110.1116184437019036No Hit
ACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCAC14080.11138112596192791No Hit
CCCCTGGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATT14050.11114380822195222No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC13870.10971990178209802No Hit
GGCTTCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGG13850.10956168995544754No Hit
CCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTG13840.10948258404212231No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA13830.10940347812879708No Hit
GGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGG13800.10916616038882138No Hit
CTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGC13740.10869152490886999No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC13680.1082168894289186No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT13500.10679298298906441No Hit
GACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGGAAGTT13380.10584371202916161No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCA13280.10505265289590926No Hit
CCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGA13160.10410338193600649No Hit
CTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGG12910.10212573410287565No Hit
ACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGC12690.10038540400972053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)