FastQCFastQC Report
Tue 10 May 2022
EGAF00003676475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250954
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG29571.1783035934872526No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG25351.010145285590188No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC25070.9989878623173968No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG24940.993807630083601No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT24290.9679064689146218No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT21080.8399945806801247No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA19040.75870478254979No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT18650.7431640858484025No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG18200.7252325127314169No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT17490.6969404751468397No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA16580.6606788495102688No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG15140.603297815535915No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC14720.5865616806267284No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA14350.5718179427305402No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT13020.5188201821847829No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC13010.5184217027821832No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT11730.4674163392494242No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG11610.462634586418228No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT11170.44510149270384214No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG10960.43673342524924885No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT10670.42517752257385816No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA10370.4132231404958678No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC10280.40963682587247063No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT10150.40445659363867487No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC9910.3948930879762825No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC9860.3929006909632841No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA9530.3797508706774947No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA9400.37457063844369887No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC9200.36660105039170526No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC9180.3658040915865059No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC9180.3658040915865059No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT9100.36261625636570843No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC8750.3486694772747197No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG8620.3434892450409238No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC8540.3403014098201264No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT8460.33711357459932895No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT8190.3263546307291376No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA8010.3191820014823434No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG7830.31200937223554914No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA7770.30961849581995105No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC7630.30403978418355554No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT7560.3012504283653578No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT7510.2992580313523594No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA7410.2952732373263626No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG7370.2936793197159639No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT7250.2888975668847677No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG7240.288499087482168No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG7180.28610821106657No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC7090.2825218964431728No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG7070.2817249376379735No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT6900.2749507877937789No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC6870.2737553495859799No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC6860.27335687018338023No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG6850.27295839078078055No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC6590.2625979263131889No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT6530.2602070498975908No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC6520.25980857049499106No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC6460.257417694079393No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT6450.25701921467679334No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC6430.25622225587159403No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC6420.2558237764689943No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT6210.24745570901440103No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA6040.2406815591702065No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG5920.2358998063390103No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC5920.2358998063390103No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG5900.23510284753381097No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGA5890.23470436813121132No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA5830.2323134917156132No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC5820.23191501231301354No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA5790.23071957410521451No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG5770.22992261530001512No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC5730.22832869768961642No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG5720.22793021828701673No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG5650.22514086246881898No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA5650.22514086246881898No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC5640.22474238306621933No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC5610.22354694485842028No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC5560.22155454784542186No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC5540.22075758904022252No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT5370.21398343919602794No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA5350.2131864803908286No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC5300.21119408337783022No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC5300.21119408337783022No Hit
AGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT5260.20960016576743148No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC5260.20960016576743148No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT5230.20840472755963244No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG5100.2032244953258366No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG4950.19724730428684142No Hit
ACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGA4900.195254907273843No Hit
CTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCAT4780.19047315444264684No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT4770.1900746750400472No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG4710.1876837986244491No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG4680.18648836041665007No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT4670.18608988101405038No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC4670.18608988101405038No Hit
CTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATG4590.18290204579325295No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA4550.18130812818285422No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG4530.18051116937765488No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA4470.1781202929620568No Hit
TAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCT4400.17533093714385903No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG4340.17294006072826096No Hit
CTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCC4320.1721431019230616No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC4190.16696286968926577No Hit
GGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGC4130.1645719932736677No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT4100.16337655506586865No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA4030.1605871992476709No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC4010.15979024044247153No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG3990.15899328163727217No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC3960.15779784342947314No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG3900.15540696701387507No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC3760.14982825537747954No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC3750.14942977597487986No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT3750.14942977597487986No Hit
CCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGG3700.14743737896188144No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC3680.1466404201566821No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA3670.14624194075408242No Hit
CCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGC3490.13906931150728818No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC3440.1370769144942898No Hit
GTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAG3430.13667843509169011No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC3420.13627995568909043No Hit
CTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAG3360.13388907927349236No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA3340.133092120468293No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG3330.1326936410656933No Hit
TTTTCGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG3260.12990428524749553No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT3220.12831036763709683No Hit
GGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGG3190.1271149294292978No Hit
ACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTG3170.12631797062409844No Hit
GTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGC3160.12591949122149876No Hit
CTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATG3150.12552101181889908No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC3130.12472405301369972No Hit
AGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCC3120.12432557361110004No Hit
AGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTT3100.12352861480590069No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG3090.123130135403301No Hit
CAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGG3090.123130135403301No Hit
GACTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCA2990.11914534137730422No Hit
CCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGC2940.1171529443643058No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG2910.11595750615650677No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG2900.1155590267539071No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT2890.1151605473513074No Hit
CTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCC2880.11476206794870773No Hit
TTTGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC2870.11436358854610806No Hit
CCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGT2840.113168150338309No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG2810.11197271213050997No Hit
ACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCG2800.1115742327279103No Hit
CACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGAC2790.1111757533253106No Hit
GTATCAACGCAGAGTACATGGGAGCCCACCAGACTCAGAGACGGAACCAG2740.10918335631231221No Hit
CATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTG2720.10838639750711286No Hit
GCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGC2700.10758943870191351No Hit
GAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCC2700.10758943870191351No Hit
ATCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGA2660.10599552109151478No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG2630.10480008288371574No Hit
CCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGC2600.10360464467591671No Hit
GTATCAACGCAGAGTACATGGGACTAGCCCACCAGACTCAGAGACGGAAC2580.10280768587071734No Hit
ATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTT2530.10081528885771895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)