FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG25.263157894736842No Hit
AAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC12.631578947368421No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.631578947368421Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAATATGCTCTGCAAATCCAATTCCCCGAATTGGATTTGCAGAGCATAT12.631578947368421No Hit
CACACACACACACACACACACACACACACACGCACACACTCACACCTGTC12.631578947368421No Hit
GTGGTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATC12.631578947368421Illumina Single End Adapter 1 (96% over 25bp)
GTACATGGGAAGGCGCGCGCGGGCGAGCTCGATCCGGTCTACGGCCGCGA12.631578947368421No Hit
CAGATGTACTTACGTTTGATATCACTGCTTCCCATGTACCTGTCTCTTAT12.631578947368421No Hit
CATACACACCATCAGTTAATTTAACCTATGCCTTAAATGTTGCATTAGAT12.631578947368421No Hit
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA12.631578947368421No Hit
GTCGCCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAAGCTCT12.631578947368421No Hit
ACGCAGAGTACATGGGAAGGCGCGCGCGGGCGAGCTCGATCCGGTCTACG12.631578947368421No Hit
TATCAACGCAGAGTACATGGGCCACTCTACCCTTGCCTCAGATTCTGTCG12.631578947368421No Hit
TATCAACGCAGAGTACATGGGTCAGGATCTTCCGACGACATTGATTGCTG12.631578947368421No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC12.631578947368421No Hit
CTGGAAAGCACGTTGGAGCTGCTCGACAACGCCGCCGAGACCGACGCCGG12.631578947368421No Hit
CTTCTCGACTTCGTCGATCACCTGTCGCAGCCGCTGCTCGAACTCGCCCT12.631578947368421No Hit
CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA12.631578947368421No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA12.631578947368421No Hit
ATCAACGCAGAGTACATGGGAAGGCGCGCGCGGGCGAGCTCGATCCGGTC12.631578947368421No Hit
GGACTCTTCTCGACTTCGTCGATCACCTGTCGCAGCCGCTGCTCGAACTC12.631578947368421No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT12.631578947368421No Hit
GTCAAGGCGAAACCGCAGCAAACTTCCTCAGCTGTCTCTTATACACATCT12.631578947368421No Hit
GTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGAC12.631578947368421No Hit
TCACAATGCTCACGAACTTCTTCTATTCGCAGTCGCTGCTGACGGCCTTC12.631578947368421No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCTG12.631578947368421No Hit
CAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAAACGAACCTTTGGGT12.631578947368421No Hit
GTACATGGGGTTGCCTTCGGCGCCGCAGCCGAAGGCGTAGGAGAGTCGGT12.631578947368421No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG12.631578947368421No Hit
GTCCATGCCCACGCCATACGCGGTCACGATCAGGATCGACGAAGGCGTCA12.631578947368421No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTC12.631578947368421Clontech Universal Primer Mix Long (96% over 26bp)
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT12.631578947368421No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTCTCTT12.631578947368421Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTG12.631578947368421No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG12.631578947368421No Hit
ATGAAGGACTGGAGTTGCTGTAGGGGTGCCCATGTACTCTGCGTTGATAC12.631578947368421No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATCTGTCTCTTATACACAT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)