FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTC36.521739130434782No Hit
GGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTT36.521739130434782No Hit
ATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC24.3478260869565215No Hit
CTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTAC24.3478260869565215No Hit
TTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGG24.3478260869565215No Hit
GGGCAACCGGGCAGCTTGGTCGCGATCTTGAGGTTGTAGAAGGCTTTGAT24.3478260869565215No Hit
GCTCGAGCGGGATGGGCCCGCCATGCTCGATGGTGAGGTACGAGATGCAG24.3478260869565215No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA12.1739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT12.1739130434782608No Hit
CACACACACACACACACAACACACACACAACACACACCCATGTACTCTGC12.1739130434782608No Hit
ACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATG12.1739130434782608No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGT12.1739130434782608No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA12.1739130434782608No Hit
ACCTACGACTTCCTCGCCTACTTCGGCCTCGTCGACAAGGACCCGGCGAG12.1739130434782608No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA12.1739130434782608No Hit
CCCGACGGCCTGGGCTCGGGCACGCCGCTCATCCAGTACGGCAGCCACGG12.1739130434782608No Hit
GGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTTAGCTGAGATTGCACC12.1739130434782608No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG12.1739130434782608No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGT12.1739130434782608Illumina Single End PCR Primer 1 (100% over 24bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA12.1739130434782608No Hit
GTTACACTGTTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGT12.1739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAGCTGTC12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAGCCGCTTGGCAGGACCATTTCTGGTCATCGGGTCCAGCGTGGCAAAC12.1739130434782608No Hit
AACGCAGAGTACATGGGAATGATACGGCGACCACCGACTGTCTCTTATAC12.1739130434782608Illumina DpnII expression PCR Primer 2 (95% over 23bp)
TTTCTACAATATACAAACCAGACACACTTAAGATCTGTAAATTAAACACA12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGCTATCCTGTCTCTTATACAC12.1739130434782608No Hit
ATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGT12.1739130434782608No Hit
CTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTT12.1739130434782608No Hit
GTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTG12.1739130434782608No Hit
ACAAACAAAACCTAAAAAAAACACATCAAAAATATACACATCTCCGAGCC12.1739130434782608No Hit
CTACTGTATCTGAAAGAAGGACACTGTAGCCGCTTGGCAGGACCATTTTT12.1739130434782608No Hit
GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC12.1739130434782608No Hit
GGGCAACCGGGCAGCTTGGTCGCGATCTTGAGGTTGTAGAATGCTTTGAT12.1739130434782608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC12.1739130434782608No Hit
CTGCTGAGATCGGAAGAGCGTCGTGTAGGGCTGTCTCTTATACACATCTC12.1739130434782608Illumina Single End PCR Primer 1 (95% over 24bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGAAGACCTGTCTCT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)