FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC36.521739130434782No Hit
TCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTG36.521739130434782No Hit
GTCGCGTCCCGCACGCAGACTGAAAGTTCACCTGCGGCCTATGCCGAAGA36.521739130434782No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG24.3478260869565215No Hit
CTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTC24.3478260869565215No Hit
TGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTG24.3478260869565215No Hit
ATGTTCTTCCTGGGCGAGATCTACGTCGACATGGAGTTGCCGCCGAGCGA24.3478260869565215No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC24.3478260869565215No Hit
TATCAACGCAGAGTACATGGGTGTGTGTTGTGTGTGTGTTGTGTGTGTGT12.1739130434782608No Hit
GATAGCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC12.1739130434782608No Hit
CTCTTCCGATCTCCCTGCTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12.1739130434782608No Hit
CTGCGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCATAAGATTTCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCG12.1739130434782608No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA12.1739130434782608No Hit
CCCTACCCTGTCCCAGGCTTGTCGATACCAGAACAGATCGGAAGAGCGTC12.1739130434782608No Hit
CAGTTAAGTAGGGCCATATGCCTCGGTAATAAGCTGACGGTGACAAGGTT12.1739130434782608No Hit
TGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTC12.1739130434782608No Hit
TCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTCTGTCTCTTATAC12.1739130434782608No Hit
GTCTTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTGTCTCT12.1739130434782608No Hit
GGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGG12.1739130434782608No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
ATATAGATTGGCGCTAGTAGATCACAGAACAACCTCCGCATACGGCAGAA12.1739130434782608No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA12.1739130434782608No Hit
CCCTACACGACGCTCTTCCGATCTCAGCAGCTGTCTCTTATACACATCTG12.1739130434782608Illumina Single End PCR Primer 1 (100% over 24bp)
GGCGAAGTCGGGCGACTTGATGACGTAGGTCTGCAGCAGCTGGAAGCGGC12.1739130434782608No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC12.1739130434782608No Hit
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT12.1739130434782608No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGAACA12.1739130434782608Illumina Single End PCR Primer 1 (100% over 25bp)
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA12.1739130434782608No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGT12.1739130434782608Illumina Single End PCR Primer 1 (95% over 24bp)
GTGGTTCATTGCGGGCGCGGCGGTCGTTTCTGCCGTCCAGCTTCAGATCG12.1739130434782608No Hit
GCAAACATGTGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCA12.1739130434782608No Hit
CTCTTTTTCAGACCTGTTGGTACCAGATAGGAAGAGCGTCGTGTAGGGAA12.1739130434782608Illumina Single End PCR Primer 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)