FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC80.38314176245210724No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT70.33524904214559387No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC60.28735632183908044No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG50.23946360153256704No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC50.23946360153256704No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA50.23946360153256704No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA50.23946360153256704No Hit
CGGCAACCTCGGCGCAGCGAGCGCGGGCGGCCAGCCAGGACCAGGGGGCG50.23946360153256704No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT40.19157088122605362No Hit
GTGCAGGAGTCACCGGCGGCGCAGGAGCAGTTGGGATCCATGGCGAGCTG40.19157088122605362No Hit
CTGCACGCTGGGGAGTTTGTTGGGTGAACTTTGAGGTGCTGATCATCACC40.19157088122605362No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT40.19157088122605362No Hit
GCCCAAGGCATTGTGGGAGTCATGATTCCAAGATGCCCGCGACGGAAAAT40.19157088122605362No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC40.19157088122605362No Hit
TCACAGTATGACTGATGATCCTCCAACAACAAAACCACTTACTGCTCGTA40.19157088122605362No Hit
GGGCTGGGCAGCCGTCCCCTTCGGTCCCCACCGTGGCCTCAGCGTGCTGC30.14367816091954022No Hit
AAAAGTAACTCACTAGAGGGAGCTTCTAAGAAACGGGGATTTTGATGAGG30.14367816091954022No Hit
GGCGTGGTCAGGTGCGTGCCTGTAAACCCAGCTACTCAGGAGGCTGAGGC30.14367816091954022No Hit
CATTCGATAACCCTTATTCAGGCCCAGATCAGCAGCACATTTCTTGCCAA30.14367816091954022No Hit
GTTACATCAGCACACCTGTCTTGACCGACCAAAAAGTCCTTGATCTCCCA30.14367816091954022No Hit
TACCAAGACCTGCTCAATGTTAAGATGGCCCTTGACATTGAGATTGCCAC30.14367816091954022No Hit
TCATTGCTCCTCCCACTGCTCGCGCGGCTTTGGGGTTGCCTGCCAGGGCT30.14367816091954022No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA30.14367816091954022No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT30.14367816091954022No Hit
GCTAGACCCTTGCAGCAGTTTCCAGACATGTCCAGCAAAAGGTACTAGGC30.14367816091954022No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC30.14367816091954022No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC30.14367816091954022No Hit
TCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGC30.14367816091954022No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA30.14367816091954022No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTTCCAGG30.14367816091954022No Hit
TTTTTTTTTAGAGCACAAGCTTTATGAGGAATAGGAGAACACATTTTTTT30.14367816091954022No Hit
ATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGA30.14367816091954022No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTC30.14367816091954022No Hit
TCCATGATGAATCCTCTTGGAGGATCGGCTATTACCATGTATAGCTTTAA30.14367816091954022No Hit
CAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTTGCATTT30.14367816091954022No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC30.14367816091954022No Hit
GCTTGCACAGAGGTGTGGTGGGAGAGGTGCAGGCGGGAACTGGGGCTGTG30.14367816091954022No Hit
GTTGTTACACTCGGCCACAAATAAACACAGAAATAGTCCAGAATGTCACA30.14367816091954022No Hit
CTGCAGAATGGCTCCCGCAAAGAAGGGTGGCGAGAAGAAAAAGGGCCGTT30.14367816091954022No Hit
GCTTTCTGCTGCCGCCCGCAAGCAGAGGGACTCGGAGATCATGCAGCAGA30.14367816091954022No Hit
CATTTGAATAGTGCTTTTATGTGTCTTTAAGAATTGTTCAGCTACAGGCC30.14367816091954022No Hit
TGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCAC30.14367816091954022No Hit
GTCTTCAGGCTGGAGTGCAATGGCGCGATCTCGGCTCACTGCAACTTCTG30.14367816091954022No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA30.14367816091954022No Hit
CATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGG30.14367816091954022No Hit
AACCTCCCTTGGGCTGTAGAAAAGAACATAAGTCCAGCGAAGATCCAAGA30.14367816091954022No Hit
GTACATGGGTCCCCTGAGCCTGTCTGAGGGGCCAGCCCTTAGTGCATTCA30.14367816091954022No Hit
CTGCATCACTGCATTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAA30.14367816091954022No Hit
GCTTCTATCTGTGCACATTTAACTTGCAGGTTTTTGAGAGCATTCACTCG30.14367816091954022No Hit
GTCGAGGAGCCGGGGCGCGGGCCTCAGACCCTCAGGGCCCACGAGAAGCC30.14367816091954022No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT30.14367816091954022No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG30.14367816091954022No Hit
CTCTTCCTCACCATCACCTTCTTCTTCCTCCTCCTCTTCCTCCCCACCTT30.14367816091954022No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT30.14367816091954022No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG30.14367816091954022No Hit
TGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGT30.14367816091954022No Hit
TCCTCCTGCCGTATCCGCCTCTCCTCCCGCTCACGCATGGCCTTCTCCTC30.14367816091954022No Hit
GGTAGAAAATGGAAGAGACGCCCCTGCTAACGGGAATGCTAATGAGGAAA30.14367816091954022No Hit
CTACAATGCAGACTCCTCTTCCAGAGAAAGGTCTTTCAGAGACAGGGAAC30.14367816091954022No Hit
TTACAGGTGTGAGCCACCGTGCCCGGCCACCAGAATCCTTTGGTATAGCC30.14367816091954022No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT30.14367816091954022No Hit
CGCTATGTGGTGATCGGCATCGGCATCAACATCGGCGCGCGCGAGGGCGA30.14367816091954022No Hit
CTTTGCAACTCCCTGGGTAAGAGGGACGACACCTCTGGTTTTTCAATACC30.14367816091954022No Hit
GCGCGGGTCAGCCCTGGTTCGCCGGCTTCTGGGTCTTTGAACAGCCGCGA30.14367816091954022No Hit
ATCCTGTATGCTGCTACCACCAAGCAGCCTCCTAAGCCTATAGGCACCAG30.14367816091954022No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCG30.14367816091954022No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA30.14367816091954022No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG30.14367816091954022No Hit
TTCCGGGACTGTGGAGGGAGCACAGGCGGGGAAGCTCCACTTCAGACAGG30.14367816091954022No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC30.14367816091954022No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA30.14367816091954022No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA30.14367816091954022No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT30.14367816091954022No Hit
GGGCTGCATGAGTCTGCAGAAGACCCAGGCGTAGCTCATGAGGGCCACGC30.14367816091954022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14367816091954022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)