FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44.0Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGTGAGTTGAATACACACACCACAAATAAGTTACTGAGAATTCTTC33.0No Hit
GCGTGAACGGGCAGCCCGGGCGCCATAACACGACGACGCCGCCGCCCTCG33.0No Hit
CTTATAGTCCAAAGCAGAAAAATTGCCTGGTGAGCTCATTAAAACATAGT22.0No Hit
GATGCATCATATTTCATTTTTCTTTTTTTTTTTTTTTGAGATGGAGTTTC22.0No Hit
AATCTAGACAATACGCCGCGCGTCAGGATAATTTGAAGAGCCAATTCACA22.0No Hit
GGAATGGAATGGAATGGAATGGAATGGAACGGAACGGAATGGAATGGAAT22.0No Hit
TCTCAATGGCGTGCCGATGACGGCGCACATCCCGCTGCTGCGCGACTGGC22.0No Hit
GTGCATAGACACTACTCGTTGTAGTGTTGGACATAGACATCCAACAGTCA22.0No Hit
GGCCGCCGCCGCAGGCGCAGCAGCAATCCGGCACTGCGGGGAGCTGACCG22.0No Hit
GACGTCATCTTCTCATCGGGGGCGCGAGCCTCTTCGCCCTGTCGGGCTGC22.0No Hit
GTTCCCCGACGGGTGGAGCGGGCGGTGGGAAGTCGACCAGACCGTCCGCA22.0No Hit
AGACGGGGTTTCACCATGTTGGCCAGACTGGTCTTGAACTCCCAGCCTCA22.0No Hit
GGCCAAGATGGTGAAACCCCATCTCTACTAAAAAATACAAAAATTAGCTT22.0No Hit
CCATGGTTACCTTTGGATCACAGCTTTTCCCCATACAGCTATATGGCTGC22.0No Hit
GCCTAGGTACAAAAATGCCAAGTAGCTTCCTGGCTGTCCGGAGTGCTTTG22.0No Hit
GTAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCAC22.0No Hit
GCATTATCTCTGTTCACTGCAGCCTCAACCTCCCAGGCTCAAGCAATCCT11.0No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT11.0No Hit
ATGTTCAACTCTGTGACTTGAATGCAATCATCACAAAGTAGTTTCTGAGA11.0No Hit
GGGTAAGGTCGGTCTGGAAGTCCGGCTCGTTGCTCTTGGGATCGAGCGGC11.0No Hit
CTTCAGTTATGCTTTACTTACTCAGTTCCTTCACCATTGTCTTCTGAAAA11.0No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAA11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCGTAC11.0Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCGGAGTAGCTGGGACCACAGGTGTGCACTACCACACCTGGCTAATTA11.0No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGAGTGTCATCTGAGACAAGGCAAGTCCCTTCCACCTATGAGCCTG11.0No Hit
GCTCCAGGCCCTGTAGAACTGCCAGGGAATAACAGACACAAGGTCAGCAA11.0No Hit
CTTGATCGCCTGAGCGGGGACCCCGGTGGCCGTGGCGATGGAGACGATCG11.0No Hit
GGCCAAATGTAATCACAAGGGTCCTTATAAGTGAAAGAGGGAGGCAGGAG11.0No Hit
GCGAAACAGCGTGCGCATCATTCGGCCGTGATGTGGGCGGCTTCTATGAT11.0No Hit
GGCCTCATGGCACATGCCTGTAGTCCTAGCTACTCCGGAGGCCAGGGCAA11.0No Hit
CACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGT11.0No Hit
GTGCTGACCATGTCCACTTCGAACTCGAAGAGCTCGGAGAGTCCCTCCCG11.0No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.0Clontech Universal Primer Mix Long (96% over 26bp)
TTCATAAAGTTCAATTTATTTCATGCTTTCCATGATAATTATGATGCTGT11.0No Hit
GAAACACTCTTTTTGTAATATTTGGAAGGGGAAATTTGCAGCGCTTTGAG11.0No Hit
ATCCTGAGGAGTCAAAGGGTTGGTTTTGTGAGCAGATGGGTGGCAGACAA11.0No Hit
ATATGATGTTCATACATTTACTTGCTGTCTATCTCTCCCCACCAGAACAT11.0No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACTATGCAGGAAAGAATTACCACTACCAAGAAGGGATCTATCACCTCT11.0No Hit
ATTGTGGATCGTTGTGACTGCGCCCGCGGTGTACCTCGACCACGCTGCCA11.0No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTA11.0Clontech Universal Primer Mix Long (95% over 21bp)
GCTCAAGATGAAGCTGAAACAACAGCGAGTGTCACCCATCCTCCCTGAGC11.0No Hit
GGTTAATTCCATTCCATTCCATTCCATTCCATTCCATTACAATCGAGTTG11.0No Hit
GGTGTACGCCACCACGTCTGGCGAATTTTTGTATTTTCAGTAGACGGGGT11.0No Hit
CCTCCGTGGAGCTCGTCCAGGCCCACCTGGACAGCATCGCGGCCCACGAC11.0No Hit
AGGCAGGTCCGTGGACTTTCTTACTTTCCACATTTCCAGTAGCCATGCTA11.0No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCT11.0No Hit
ACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG11.0No Hit
CTCGAACGCCTCGTGGCCGAGCGCCGGGGCGGGCCGGTGTGGGCCGAGGC11.0No Hit
CGCTGGTAGTAGGCATTGACCAGGAAGCGGGCCACGCCGGGCACCCCGTA11.0No Hit
CAACTGTATGATTCAGGTTGTTGAATACAAGACACACAGGTTTTGGCTTT11.0No Hit
GTCGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATAAGATAGAGTCTTCGTGAGCACAGTGGGTGTCAGACTTGAAGTTTG11.0No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAAAGCGGACCATCGCGTATG11.0No Hit
ACATACTACAGAGTGAAAAATAAAGCCACCAAATGGAAGACATTTGCATT11.0No Hit
GGCATCTCCAAGGGCGTGCCTACGGGTTTCACCGAACTCGACGAGCTGAC11.0No Hit
GCTTGGGAAACAGAGTGCCTGTCTCTACAACAAATTAAAAACTAGCTGGG11.0No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGATATAATAGCGGGGCTGGACGTGGTGGCTCAGACCCATAATCCCAACA11.0No Hit
CATCAGAATAGTGAATATGTATTATTTAGTCCTCCCAGGTGTCAACCCTG11.0No Hit
GTTATATAGAAAATATTAACCAAAATTTTAAATGACTCAGACTCTTGCAC11.0No Hit
GACATCATCCACGCCGGCTTCGTCATGCACGACCTTCTTCCCGCGGAAGA11.0No Hit
ATTCTGTTTCCCTTCTTTCTTTCTTTCCTTTGTTGGCTCCCTTCTGCTGA11.0No Hit
GTTATCTGGCCACTCAAAGTAGAAGCTCATCTGTGAGAAGGAGAAATAGG11.0No Hit
ATCCTCGAGCAGGCCGACACCACCGTGTGGCTGGAGCCCGGCTTCCATGC11.0No Hit
GATTTGAATGGAATCATCGAATACACTCGAATGGAATCTTCATCGATAGG11.0No Hit
CTGATGTCATCATATAGGTGTTAGGAATTTCTTGATAAATTAACTTCTAG11.0No Hit
CTACATGCCCAAAGGCGGCTTTGGAAATCTCATCGCGCTCCCGTTACAGA11.0No Hit
ACCCAGGAGGCGGAGCTTGCAGTGAGCGGAGATTGCGCCACTGCACTCCA11.0No Hit
CCCTCAAAAACAGCAGTTTCCCCTTGAGGAGCCAAAGAGCAAAGCCAGGC11.0No Hit
ATGTTGCAATTTCCCCATGGGTACCACAAAAGTGGTGATGTGTGCCTGGT11.0No Hit
GCCCGAGCACGCCCTCGCCCGCATCCTTCTGCCGTACCTGCGCGCCGGCA11.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)