FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55.0Clontech Universal Primer Mix Long (96% over 26bp)
GTTCGGGGACGTGCGACGGATCGGCTCGGCGGCGCTGGACCTGTGCATGG33.0No Hit
ATGTTAAACACCCTTTTTGTGGAATTTGCAGCTGGAGATTTCAAGCGCTT33.0No Hit
CGCTGGCAGAGTGGCGCGGGCCGGACGCGGGTCTGCAAGCGCTCACCTCG22.0No Hit
AGATACACCTCTAAATTTCAGTACCTAATTCACAGGTGGGGATTCAAGCC22.0No Hit
ATCTTCAGTCTACTACCAGCCCTTTTGTTATGCCTGCCAGTTAATCCCAG22.0No Hit
GCGGCACGCCCTCATAGGGGCCTGTCCACGGCTGCAACAGCTCGGCGCGC22.0No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22.0Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCATTCCATTCCATTCCATTCCACTCCATTCCATTTCACTCGGGTTT22.0No Hit
AGTTACTATTTTATGTAAGTTGATTAGAGAAGGCTTCGGTGTGAATGGCA22.0No Hit
CCGCGGTTCCGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTT22.0No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.0Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT22.0Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG22.0Clontech Universal Primer Mix Long (96% over 26bp)
CATATGTTGTATGCATCCCCCAACCACAAAATATGCACTCAATATGAAAC22.0No Hit
ACTCTGGGTAGTGTTTATTCAGGGAGAACAAGTGAGAAACTCCATCTCAA22.0No Hit
CGCCAGCACCGCGGCATCGACGAGGTCGGCGGCGACGCCGTGCTTGATGA22.0No Hit
ACCCAAGATCGAGCCAATGGCCTAGACGGGCCACCCGCTAAAAGAAGCCG22.0No Hit
TGGTAAAATTGAGTAATAATAGATGGATGACCACTTATCTCTTTGTATAA22.0No Hit
GAAATGCTCCGGACGGAGCGTGTCAAAACGCCTTCACGCCCCGGCGCAAA22.0No Hit
TATTACATCTTTTTGCTTTTTATTTTGTTTTGTTTTTTGAGACAGAGTGT11.0No Hit
AATCATAGCACTGCGTCTTGGTGAATTGATTCTAGAGGTGGAAGTTAGTG11.0No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACG11.0No Hit
ACATCCACCTGCGGGCCGGGAGTGCCCCTGCCGCGCGGGTCAGCGGCGAG11.0No Hit
GAATGGAATCGTTCCGAGTGGAATAGGAGGGAATGTATTCGAGTGGAATG11.0No Hit
GGTTGACACCTGGGAGGACTAAATAATACATATTCACTATTCTGATGCTG11.0No Hit
GAAGACACCTTGACTTTTTGAGTTGCCAGAGTTTTTGCACTGGTTCTTTC11.0No Hit
CTCGTGGGCCGCGGCATCCAGCCCGTCGTGCACGAGCCAGGGCAGGTGCT11.0No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTCGGCGTCACGGCGGGCCCAGAAGATGCCCTTGACGGCGAGGTTGTC11.0No Hit
TGGCTCCAAGCACCAAAGAATCAATTGGCATATATCTACCAGAACTCAGA11.0No Hit
GGCTCTCTCTTTCCTTGACCAGTCAACAGCCCCAAAGATACGGCTAAAGA11.0No Hit
CTCTTAAAAAAAAGAAAAAAATTGATAAGACAAGCTGTTATAAGGCTTGC11.0No Hit
GTTCAAGACCAGCCTAGCCAACATGGTGAAACCCCATCTCTACTTAAGAT11.0No Hit
GGGCCGTCCCTCCATCACGGTGGTCGCGGCGACCACCCGCGTCGGCAACC11.0No Hit
CCCCTAGAGGGTCCCGATTACCCATCGCCTGGATAATAACGGGCTGGATA11.0No Hit
CACTGGGGGCGTATCTTTCCCATTGGAAAAGTGTGGTTATTGGAAGTTTC11.0No Hit
ACGTTATTCAGCCCAGGAAAACAGTTATATAAAATCACTGGTTCCCTGAG11.0No Hit
AAGATATCCCGTTTCCAACGAAATCCTCAAAGCTATCCAAATATCCACTT11.0No Hit
CCTTCAGCGCGCGCGCAACGGCGGCCACGCGCTCCGGCAGCAGCAGCGCG11.0No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.0No Hit
ATTCGACAATGATTCCATTCGTGTCCATTTGATGATTCCATTCATTTCCT11.0No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0No Hit
GCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAG11.0No Hit
GGTTTCCCTTGGATCTCTGACACCTGGGTATATCCACAAGTGTCTATGCA11.0No Hit
ATTATTTGATGATTTTATAAATAGTCATCTCTTTCCTCATCTTTCCAGTC11.0No Hit
GGGGTAGTTAATGAGATCAACCCTGAGGGTAAGTGAGTCTAAAATAGTGC11.0No Hit
AGACCGGCTTTTGGCTGGAGGAGTTCGCGGCTCCCTACTATACCTTCAAG11.0No Hit
ATATTTTTATGTGTAAGTACCTCCACACATTTACTCCCACATGTTCACAC11.0No Hit
GGACATAAGAGATGCCTGGCCTTTGCAAACAGTGTGCCTGAAGATACCAG11.0No Hit
ATCGTGGTGCAGTACCGCGGCGATGCGCCCGCCTACACCGAACTGCTGGC11.0No Hit
GGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCC11.0No Hit
GTGTAGGAGTTCCAGTTGTTCTGTATCCTAACTAATGCTTGACATTTTCT11.0No Hit
TGGCAAGACTGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCC11.0No Hit
CAGTTCACTGCAACCTCCACCTCCCAGGCTCAAGCCATTCTCTTGCCTCA11.0No Hit
CAGCCATGCATCCCCATTTCTAACTCTCCTGCCTCCCTCTTTCACTTATA11.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTAGCTCTCTGGAAGTCTGTGGCGTCAGGTCTCATCTTGGCGACAGGA11.0No Hit
TCTATATAGTGCTTGACCTGGTGTATACTCTCCCAAAGGAAATCATCACA11.0No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGACG11.0No Hit
GTTTCTTTCTAAAGAAGAGTCTTGCTTTGTTGCCCATGCTGGCATGCAGT11.0No Hit
CCTTTTCAAAGAGCAGTTTCCAATCACTCTTTCTGTGGAATCTGCAAGTG11.0No Hit
CCTTCATTCTGCCAACGAACCCGATGAGCAACGAAGAAAAGGAATCGGAC11.0No Hit
GCTGAGCGACGAATCCAGCGTTTCTTCTTCCGCGGGAAGAAGGTCGTGCA11.0No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAT11.0No Hit
ATATCATTCTGCCCCTGGCCCCTCCAAATCTCATGTCCTCACATTTCAAA11.0No Hit
GCCCAGCACCGGGTCGAAGCGCTCCATCGCCTCCGCGAAGTCCATGGTCT11.0No Hit
CTGTCAGGCTGGGCCTGTAGGATACGATCCAAGAGAATGAATCAAAAAGT11.0No Hit
GTGTTTCAGAGGGAAAACCGCCCTCTCACACCCCTCGCGTCATTTATGTG11.0No Hit
GTGGTGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCATGCCAT11.0No Hit
GGTACGGACCCTGCCCATCCGGGTCGCGCCGCTACCCGGGGAGGCCATGG11.0No Hit
GAGCCAGCCCTCGAGCATGCGGGAGGCGGCCGTGGTCGGCTGGCCGCGCG11.0No Hit
GCAACCTCCACCTCCCAGGTTCACATGATTCTCATGCCTCGGCCTCCCGA11.0No Hit
GAGTGAGACTCCGTTTCAAAACAATAAAAAATAAACATAGGCCTGGTGCA11.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)