FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGCATTTCACACTGCGGTTCCAGGCCACAGGGTCCCTTGGGTAGGG24.25531914893617No Hit
TCTAATTACAGAGTGCCTAAGGAGGTTCGGCATAGCCTTGCCAGTAAAGA24.25531914893617No Hit
ACTCCTGCTCATCCAGACTCATGGCTTTCAATGCCATCTATATGTTCATG24.25531914893617No Hit
GTGCTGGGGGGCCAGCGAGCTGATGCTCGAGGGCAGAGCCCTCAGCGGCA24.25531914893617No Hit
GCCCACGTTGTCGACCACCGCCGGCGCGATGACGGTGAGCTGTCCCACCG24.25531914893617No Hit
GTACTTGCCGCCCTTGTTGTCGCCGATGCGAATCACGCGCTCGGGCGGCA24.25531914893617No Hit
CTGTAACCTCTGCCTCCCGGGTTCAAGCGGTTCTCCCACCTCAGCCTCCC24.25531914893617No Hit
CCTTGGACGGATTTGACTCGCCTTAGGGCGACGAGCCTCTCTTAGGATGG12.127659574468085No Hit
GAAAATATGCAGCATTTGGCCGGGGCGGTGGCTCACGCCTGTAATCCCAG12.127659574468085No Hit
GTGCATGAGCGCGAGATGGCGCGCGTGGTGCAGGCCTGGCTGCTGCCCGT12.127659574468085No Hit
TTTGGGAGGCCGAGGCGGGCGGATCATGAGGTCAAGAGATGGAGACCATT12.127659574468085No Hit
CCACGCGCGTGGTGCCCAGCACCAGCGAATGGCCGACGCGCTCACCCAGT12.127659574468085No Hit
TCTTTTTGCTTAGCTGTAATTTCCAGATTTTCAGCAGAGCATCCCTCCTT12.127659574468085No Hit
GGGCTGGGTGTGGCGCGCCACGTCGCGGAGCGCCTGCTTCAGCATGGACA12.127659574468085No Hit
GCCAGATCCGCACCAGCGCGTCGCGGGAAGGCATCGGTGCATTCTGCCCT12.127659574468085No Hit
CCATAGATTGTCATCAGGTAATGCCCATTTTGTACACATTCATTCATTCG12.127659574468085No Hit
CTCCAAGAAGGAGCGCATGCTGCAGAAGGGCTCCGGCTTCCACCTGGACC12.127659574468085No Hit
CTGTGACTTGAATGCAAACATCACAAACAAGATTCTGGGAATGCTGCTGT12.127659574468085No Hit
GACGTGGAGGCGGCCCAGAGTGCCGTCGCCCAGCAGACGGCCGAACTGCG12.127659574468085No Hit
GGAAGAACTCGTCACCGTTCTCCAGCAGGCGGGCCGCGTTGCCGTGCGTC12.127659574468085No Hit
GGGAGTGGGTCCCGGATCCAAGGTGGGCCTGCTGCTGTGGAACGGGCTGC12.127659574468085No Hit
GCTGGGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAG12.127659574468085No Hit
GGACCTCGTGGCCCTGGCGGCGGGCCAGCTTGAAGGCGGCCTCATTGGCC12.127659574468085No Hit
GACCAGAACTTTGTGTTTTTGCAAGGACTGGAGTTACCTTTCGGCTCTTT12.127659574468085No Hit
ATCATGTCATCTGCAAACAGGGACAATTGGACTTCCTCTTTTCCTAATTG12.127659574468085No Hit
CACCAAGGTCGAGGCTGCCCTGCGCCATGGGCTCGCCCCCTGTCTCTTAT12.127659574468085No Hit
GTTCAGCTCTGAGGCCAGATCGACGGCTGCAAACCTCAGATCCCACCAGA12.127659574468085No Hit
CATCTTTACCTACCAGAAACTCACCATCTAGGACGCGCAGGGTGCTGGAG12.127659574468085No Hit
ATCATGAGGAGATACAAGAGGAAGGCTCGAAGTAAGAGGAGGAGCCCCAG12.127659574468085No Hit
GTGCGAGTCGAAGTCGGTCTCGTAGCCGCGCGCCGCGAAGCGCGAACGCT12.127659574468085No Hit
CTTGTCTCCCAATCTGGAGTACAATGGCGCGATCTTGGCTCACCGTAACC12.127659574468085No Hit
GTAAAGGAATGCTCCAAAAACTTGGATCAAAAAAACATTTTTGCCGGGTG12.127659574468085No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GAATTTTTGATCTATGTACCCAATGCCTTTACACCGAACGGGGATGCCCA12.127659574468085No Hit
CGTGATCGGGCAGAGCCCGAGGCCCGGCGCCGTCCGGCGCAGGGGCTTCC12.127659574468085No Hit
GAAGGCTACGTGACGCTCGAATTCAGCAACACCACGCCGCTACCCGCCAA12.127659574468085No Hit
CGCGGCGAGGAACAGCAACCACACCGGGATCACCGCCTTCACCTCGTACG12.127659574468085No Hit
CGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGA12.127659574468085No Hit
CAGTAATCCCCTCACACTCACACTCATGCCTCCCTCGTGCTCACCCTCAC12.127659574468085No Hit
AAGGAGGCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)