FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTGCATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAA24.25531914893617No Hit
GGTGATGCTGTCGACCGGCGTGGCCGACGCCTCGGGCCGCTACCAGTTCG24.25531914893617No Hit
CTGCGCGCCGGCGGCAAGCACAACGACCTCGAGAACGTGGGCTACACGGC24.25531914893617No Hit
ATGTGGAACAGAAGTGTGGCTGCTCTGGTGGAAGTGGCAATGGTAGTCCT24.25531914893617No Hit
GGCTCGCCACCTCCGAAGTGGCACATCAACTACATGAAGGCAGATCGCAG24.25531914893617No Hit
AGTTTACACTGTTTCTCCAAGCCAGCACAGCTGATATCTCCTGGTGCTAT24.25531914893617No Hit
GTCAAGGATGACTTCAAGTTGTTGGGCTTGAGCAACTGGAAGGATGGAGT24.25531914893617No Hit
TTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTC12.127659574468085No Hit
CTGCATGAATTCGTGCTTTCATGGAGGCCCAGGGGCCGGGCAGCACAAGT12.127659574468085No Hit
ACTCTGCCCAGGACGCCTCCGCGGAGGCCCTGCTCGAGCAGTACTCCCAG12.127659574468085No Hit
GTAAAAAACACATGGCTGGGCGCGGTAGCTCACACCTGTAATCCCAGCAC12.127659574468085No Hit
GACGGGAAGTGCGCGCAGCCTGGGAGCGCTCAGGTCTTCGGCAGCGTGAC12.127659574468085No Hit
CAAGCGGCGCATCCTGGCGGAGAACGGCATCCTCACTCGGCACTACGCGA12.127659574468085No Hit
GTGCGCGGGCGCGGCGCGTGTCCGCGTCGAGCACCGCGCTCACGAAGGCG12.127659574468085No Hit
GCCTCAGCCTCCCAAGTAGCTGGGACTACAGGTGCCCGCCACTACGCCCG12.127659574468085No Hit
GTGCCGTTATTGCAGCAGCGTTGGCCGCATTTTGCCGAAGCAACGGCTCG12.127659574468085No Hit
GAAGGAAAACTCCAGCCACCTCAGTAAAGCAATAGTGTTTGCCATTCAGG12.127659574468085No Hit
TTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGATCTCGAACTC12.127659574468085No Hit
GCCAATATCATACTGAATGGGCAAAAACTGGAAGCATTCCCTTTGAAAAC12.127659574468085No Hit
GGAGCGTCCGCACATATTGATGAGAGTGAGTGTGTTAGTGGTCGATGGGC12.127659574468085No Hit
ATCCTCAGCGGCGCCATTTTTGTCAAGTGTCCCTTTTCGCCGTGTCTTTC12.127659574468085No Hit
TCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGGCGCCCGCCACCA12.127659574468085No Hit
GGGGCGAGCCCATGGCGCAGGGCAGCCTCGACCTTGGTGCTGTCTCTTAT12.127659574468085No Hit
CTTTTTGGTATGCCGCTCGCCGAAATGGTCGGTCACATCGATCACATACA12.127659574468085No Hit
GGCGCGTGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTGGAGATGGG12.127659574468085No Hit
GCAGTGCTTTCAGTTAAAGAACCGATGCTGGCTATAACTTCTCTTCTGAA12.127659574468085No Hit
GGTAGCCGCCGGGCGTCTTCAGGCCCTTGGCCACGCGCACCAGCAGGTAG12.127659574468085No Hit
CGAGAACGCTGAGCGGGGCGCCGCTTGGCGCCCCGCTCGATTCGTTACTG12.127659574468085No Hit
GTCTATGCCACATGGGGAGGATGGTTGCTCCCTGAGGGCAGAAGGCCATT12.127659574468085No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT12.127659574468085No Hit
ATCTTGTCTGACAGGCAGGCCCAGACGTTATCCTTGACACGAGGAATAAT12.127659574468085No Hit
CGGCCCACCCCGACGCGGTGAACGACCCGACACGGGTACGGTCGGCGCGC12.127659574468085No Hit
GGCCGATTTGTGGATGTGTTCAAAGGGAAGCCATGTTTAGACAAAGAAGT12.127659574468085No Hit
GGTGACTCCCATGTACAGGAAAATTCCAAAGAGCACGGCCAGGGGGATCT12.127659574468085No Hit
CCCCAAGTGTTGGCATTTTGCCATTAGAAACCCCAAGTCCCGGAGACGGA12.127659574468085No Hit
ATGGCGGCGCAGCGTGCGGGCCAGATCCAGCAGAGAGTGATCGATCACGA12.127659574468085No Hit
CGCCTGCTTCTTGCCGAGCTTCTTCGGTTTGTCCTGTCCCTTCATGTATG12.127659574468085No Hit
GACGAGGTCGACGTCAGCCTGCCACTGGGTGAGCGCGTCGGCCATTCGCT12.127659574468085No Hit
GGGATGTTTCAATTGAAGTCACAGTGTTGAACAGTCCCTTTCATAGAGCA12.127659574468085No Hit
TCCCTACACGACGCTCTTCCGATCTCATGCAGCAACTCCAGTCCTTGCAT12.127659574468085Illumina Single End PCR Primer 1 (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)