FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53.1847133757961785Clontech Universal Primer Mix Long (96% over 26bp)
GACAAATGCTCTTTCCCTAAACTCCCCCCATAAAAAAACCCGCCGAAGCG42.547770700636943No Hit
CACTAGGAATTGCAGCCACTGGGCAGTTGGGGTGGCTTCCTGGGAGATGA31.910828025477707No Hit
TTACTGGGAAGTGGACACTAGGAATTGCAGCCACTGGGCAGTTGGGGTGG31.910828025477707No Hit
GCATGAATGTGCACACAACTGCTGGAGGGGCCTCCACCCACCCCCATGCC31.910828025477707No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATG21.2738853503184715Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGAGCCAAGGACAGTTTCCTTGACCATGTGCCATGCAGAGAGCTGGC21.2738853503184715No Hit
CGATGAAGTTGGAGCCTGGGATCGGGTCACCGCCGATGCCGACGCAAGTC21.2738853503184715No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA21.2738853503184715Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGGCTTTCACTCCGCGCTCACGGCGGCGGCCAAAGCGGCGGCGAC21.2738853503184715No Hit
ACAGTGAGCAGCTGTCAGCACGAAGTCGTCTTGTATCAGGAAGCCACCGC21.2738853503184715No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTT21.2738853503184715Clontech Universal Primer Mix Long (96% over 26bp)
GATCTTGCTGACCTCAGCCGGCATACCGGGGGTGTCGGCGAACGACTGGG21.2738853503184715No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.2738853503184715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGC21.2738853503184715No Hit
GCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACC21.2738853503184715No Hit
GGTATCACCATGGGGGCGTCATGGGCTGAGGATTCTGCAGATAGGACATC21.2738853503184715No Hit
TTTTTGAGATGGAGTCTCGCTCTGTTGTCCTGGCTGGAGTGCAGTGGTGC21.2738853503184715No Hit
GCCACCCCTCGGACTCGCTCACTCGCCTGCCTCCTCTGGAGCCGCTGCGA21.2738853503184715No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.2738853503184715Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG21.2738853503184715Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAGGGATCGGCCTCCACCCGCTCGACGATGCGGTCCATCATCGCCA21.2738853503184715No Hit
TCCCATCCCCAACCTCTCAAGCCATGTGGAAGGGGCTTCACCGATTGCAA21.2738853503184715No Hit
GGGTCTCGGCGGCAGAGTGGCTCCCCAACAGCTTAGCAGCCAGCAGACCG21.2738853503184715No Hit
TTCTCCGCGAGCGGCATCGCCGCCGCGAGATTGCCGGTGGCCACGTAGCC21.2738853503184715No Hit
GCTTGCAGTCGCGCGTCTCGTCGCGGGTGACGACGCGGCGCGCGAGGCGG21.2738853503184715No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG21.2738853503184715No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.2738853503184715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCGCGCAGGTCCGTCTCGATGGCCCGGCGCCGGCGCACGCCCGCATCC21.2738853503184715No Hit
ACGCAGAGTACATGGGGCCATCCATCCAACCTTTAACTTGAAGAGCCTTT21.2738853503184715No Hit
GTCGCTCACGGTGATCGACCAGGGCTGCGGGATGAGCCCGGCCTTTGTGC21.2738853503184715No Hit
CCTTTCTTCCTCCTCAACATTTGGAACTCAGAGCTGGCATAAAACCTTGT10.6369426751592357No Hit
GGCTGTTCTTGGGGAATGGGGCCTGGAAATAGCTTTGTGGAGGGCGAGCT10.6369426751592357No Hit
ACCCCGGCGTGATGGCCTGACTATGGATTCCCGTGTGCGGCAACATGGGG10.6369426751592357No Hit
CCCCAGAAGATCCCCAAGTTTTCCAGCATTTTAGGCAAAAACGAAGGCAG10.6369426751592357No Hit
CGCGAGAACGACCCGGCGGCCAGTTCTCTTCCTCCTGCGCACCTGCCCCG10.6369426751592357No Hit
CACCTGCCCCGGCCACCCCTCGGACTCGCTCACTCGCCTGCCTCCTCTGG10.6369426751592357No Hit
CATCCTGGGAGATGAGCCGCGACCAGGTCCTGGGAAGGACTATGGACTCT10.6369426751592357No Hit
CCCACAGAGGGAGCATATAAACCAGCTCTAGCCAAAGGGTAATTGAGGAT10.6369426751592357No Hit
TTCTAGGCTGGTATCACCATGGGGGCGTCATGGGCTGAGGATTCTGCAGA10.6369426751592357No Hit
CTAGGATTGCAACCCCTGCCTTTTTTTGTTTTCCATTTGCTTGGTAGATC10.6369426751592357No Hit
ATGGTACACTCATACAGAAGCTTCTCCTGATTGTCCACTGAATAGAAGGC10.6369426751592357No Hit
GGCAGAGAGGGATATGAAACACTAGGATACGCGGCATTAGAAACAGTTGA10.6369426751592357No Hit
GCACTGGCGCTTGAAGTACAGAAAGGAGCATAGAAAGAAGTCCTTCGCGG10.6369426751592357No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTCTATCCAGCTGGGGGCACCGAGACGGCCTCATTCAGGGAAGTCCA10.6369426751592357No Hit
CTCCTAAGCAGCCCTAACCACATGAGAGTGTACTTCATCACGTCGGGTAT10.6369426751592357No Hit
GAGTAGAATCATAAGCTATTTTTCCTTCTGTGTGATAGACACTTTTGCAG10.6369426751592357No Hit
TAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10.6369426751592357No Hit
GCGACTTCACCGCGGGGTGGGCACCGTGGTCGCACCTGGACGCCCAGATT10.6369426751592357No Hit
GGGCGTCATGGGCTGAGGATTCTGCAGATAGGACATCACCACGGCAGAGA10.6369426751592357No Hit
GCATGGCACATGGTCAAGGAAACTGTCCTTGGCTCAGACAGACCTGGGGT10.6369426751592357No Hit
CAGTAATGCTTTCCAGAAGACAGGGCCTGGGAACATAAGACCTGGCTGGT10.6369426751592357No Hit
GTACTTGGGGCTTTCACTCCGCGCTCACGGCGGCGGCCAAAGCGGCGGCG10.6369426751592357No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCTATCCAGCTGGGGGCACCGAGACGGCCTCATTCAGGGAAGTCCAG10.6369426751592357No Hit
CAAATACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC10.6369426751592357No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
AAACTGAAAAGTCCAGGAGTAGATTTCACTTCAGGCATGACTGGATCCTG10.6369426751592357No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTA10.6369426751592357Clontech Universal Primer Mix Long (96% over 28bp)
GGCGGCGCGAGAACGACCCGGCGGCCAGTTCTCTTCCTCCTGCGCACCTG10.6369426751592357No Hit
TTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTG10.6369426751592357No Hit
GCTGGGCCTGCTGTTCGTGCTCTCCGTCTATTACTTTCCCACCGGCATCG10.6369426751592357No Hit
GGATATAATCTCCTGGTGCGCCATTTTTTTAAGGCTGTTGGAAAATTGCA10.6369426751592357No Hit
AACATGGACAGCATCTCCCCCAGCAGCAGGCAGGGGAAGGGAGGCAGGAG10.6369426751592357No Hit
GTCACCCAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCACTGCAGCCTCT10.6369426751592357No Hit
CTGCATGGCACATGGTCAAGGAAACTGTCCTTGGCTCAGACAGACCTGGG10.6369426751592357No Hit
CGCCAGCACCAGGCTGTTCCACATGGCCGGCGGCCCGATGGGGGCAGCCC10.6369426751592357No Hit
CTTCCTAACTTCCCTGTGAAGTATTTTTGTTCCCAATCTGCAGGTGAAAA10.6369426751592357No Hit
TAGCTAGCGTCTGGCCTGAGAACCTCGGCGCTCCGGCGGCGCGGGCACCA10.6369426751592357No Hit
GAGTACATGGGGCCATCCATCCAACCTTTAACTTGAAGAGCCTTTCCTGC10.6369426751592357No Hit
CTACTGCTCCTTCATCAGCTTTGCCAACTCTCGGAGCTCGGAGGACACGA10.6369426751592357No Hit
CTGCGCACCTGCCCCGCTCGGTCAGTCAGTCGGCGGCCGGCGCCCGGCTT10.6369426751592357No Hit
GGGCAGGTGGTGGCGCCGCGAAGATGGTCGCCAAGCAAAGGATCCGTATG10.6369426751592357No Hit
ACCAGCAGGCCGTCGATGCGCGCATCCGGCCGCAGCTGGCGTGCGCGCGA10.6369426751592357No Hit
AATCGGTTCTTCGTGGTAGTTATCACCGATCTGCTCGACAACGAACGCGC10.6369426751592357No Hit
AACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACAA10.6369426751592357No Hit
CCTGGGAACCTCGGCGCTCCGGCGGCGCGGACACCACGAGCCGAGCCTCG10.6369426751592357No Hit
GGACCAGAGCGAAAGCATTTGTCAAGAATGTTTTCATTAATCAAGAACGA10.6369426751592357No Hit
ACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAG10.6369426751592357No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
GCTCATGACGGGCTCACGACCGACCTAGAAAGCCGAGAAGCAGAGTCCGC10.6369426751592357No Hit
GCAGAGAGCTGGCTAGTCCCCTTCCATTCCACACAACAAGAGTCCATAGT10.6369426751592357No Hit
ATCAACGCAGAGTCATGGGAAGCAGTGGTATCAACGCAGAGTCAAGGGAA10.6369426751592357Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGGGCCATCCATCCAACCTTTAACTTGAAGAGCCTTTCCTGCAG10.6369426751592357No Hit
CAGGAGGATGTTGGCCTGCCGCTGCAGGGACTTGCCAAAAGGGTGGCTGC10.6369426751592357No Hit
GTTCCCGCACTCCTTGACGATGCTTTTTAGGCCCACATCCGCATCTGCTA10.6369426751592357No Hit
GTCAACCGCAGACCCAGCTCCTTCCCAAGCTGTGCAGTGCACAGAAGGCA10.6369426751592357No Hit
GTGCCGAACACCGTGACGCCGCTGCCGCTGCTCGGCGTTGCGGCGGAGGA10.6369426751592357No Hit
CGTCAGATGTGTATAAGAGACAGCCCTAGGAAAGGGCCAGGTTCCCTCTA10.6369426751592357No Hit
GATCAAGGCTGTCTTTGGGAAGTCAGCCATGAAGCACATGGTCATCTTGT10.6369426751592357No Hit
TCCTTTGCTTGGCGACCATCTTCGCGGCGCCACCACCTGCCCCGGCCACC10.6369426751592357No Hit
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAGCCCAAGCTGCCGGCCCCCGCTTGTTTTTACATCCACTGTCAGCT10.6369426751592357No Hit
GCACATAAAGTCCATAGTTAAGATGATGATGCCATGCAGAAAAACACGTG10.6369426751592357No Hit
TTCAGGTACAAGGAACCAGGCAAGTCAGCGCAGTGTCTTTTGAGTAAATG10.6369426751592357No Hit
CGGTGGGGCGGTGCGTGCGGGGGGCTGGCCTCGGGCGTGGGGCCAGCGGT10.6369426751592357No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
TAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCG10.6369426751592357No Hit
CTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGC10.6369426751592357No Hit
ATGCTTTCCAGAAGACAGGGCCTGGGAACATAAGACCTGGCTGGTAGAAA10.6369426751592357No Hit
GTGTCCTCCGAGCTCCGAGAGTTGGCAAAGCTGATGAAGGAGCAGTAGAC10.6369426751592357No Hit
GGGCAGTTGGGGTGGCTTCCTGGGAGATGAGCCGCGACCAGGTCCTGGGA10.6369426751592357No Hit
TCTCTTCCTCCTGCGCACCTGCCCCGCTCGGTCAGTCAGTCGGCGGCCGG10.6369426751592357No Hit
CTCCGGCGGCGCGGGCACCACGAGCCGAGCCTCGCAGCGGCTCCAGAGGA10.6369426751592357No Hit
ACTTCAGGAAGCAGCTACCCCTAAAGCCCAGGGAAACACAGGAAAGAAAC10.6369426751592357No Hit
GGGGATGACCGTAGAGTGGGATCAATGAGGGTCTTGAGAAAGAAGAGGAA10.6369426751592357No Hit
TATGTTCCCAGGCCCTGTCTTCTGGAAAGCATTACTGGGAAGTGGACACT10.6369426751592357No Hit
TGATGTCACCGGCAAATTCGTCGAGTGCCGCGATGGACATGGCACCGGCG10.6369426751592357No Hit
GACAATGCCCTGGGCCACCTTGTTACACACAAGAGGGCCTCCAGAGTCCC10.6369426751592357No Hit
GGTCTGCGCTGAGTGCTCGCGCCAGCGCTTCCTGCTCCCGCGCCTGTCCC10.6369426751592357No Hit
GCTGAAGTGGTGTGCTTGGGTCGCTGTCTACTGCTCCTTCATCAGCTTTG10.6369426751592357No Hit
GCCCGGGTGGAGTCCCGGCCGGAGCTGGTGGTTCGGGGGCGGTGCTAGGC10.6369426751592357No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.6369426751592357Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAGGAGTCAGCAAATGCCTTGAGAGAACAGTATGCACAGATGTTGGG10.6369426751592357No Hit
ACATCATGTGGAAGCTGCCAAGGCTTGGTGCTTTCACCCTCTGAACCAAC10.6369426751592357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)