FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4831
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT250.517491202649555No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT200.4139929621196439No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC160.33119436969571514No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG150.31049472158973296No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA140.2897950734837508No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG140.2897950734837508No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT140.2897950734837508No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA140.2897950734837508No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG140.2897950734837508No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA140.2897950734837508No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA130.2690954253777686No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC130.2690954253777686No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC130.2690954253777686No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC130.2690954253777686No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA120.24839577727178638No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG120.24839577727178638No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT120.24839577727178638No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT110.2276961291658042No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT110.2276961291658042No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG110.2276961291658042No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG110.2276961291658042No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC100.20699648105982196No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA100.20699648105982196No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT100.20699648105982196No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC100.20699648105982196No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT100.20699648105982196No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC100.20699648105982196No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC100.20699648105982196No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG90.18629683295383978No Hit
AGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCA90.18629683295383978No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT90.18629683295383978No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG90.18629683295383978No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA90.18629683295383978No Hit
GGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACC90.18629683295383978No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA90.18629683295383978No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG90.18629683295383978No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC90.18629683295383978No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC90.18629683295383978No Hit
CACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGT90.18629683295383978No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA80.16559718484785757No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA80.16559718484785757No Hit
GCTGGTAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCA80.16559718484785757No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG80.16559718484785757No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG80.16559718484785757No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT80.16559718484785757No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG80.16559718484785757No Hit
TTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTA80.16559718484785757No Hit
CCCTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGT80.16559718484785757No Hit
CCCCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGG80.16559718484785757No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC80.16559718484785757No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT80.16559718484785757No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGGTCAGTGG70.1448975367418754No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG70.1448975367418754No Hit
GTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCA70.1448975367418754No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT70.1448975367418754No Hit
CTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCC70.1448975367418754No Hit
GAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCG70.1448975367418754No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG70.1448975367418754No Hit
TTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACCATCTCCGTGT60.12419788863589319No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA60.12419788863589319No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC60.12419788863589319No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC60.12419788863589319No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG60.12419788863589319No Hit
GCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACG60.12419788863589319No Hit
CTGCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTG60.12419788863589319No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG60.12419788863589319No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA60.12419788863589319No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC60.12419788863589319No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG60.12419788863589319No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG60.12419788863589319No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT60.12419788863589319No Hit
CTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAG60.12419788863589319No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG60.12419788863589319No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT60.12419788863589319No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA60.12419788863589319No Hit
ACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGG60.12419788863589319No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA60.12419788863589319No Hit
CTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACA60.12419788863589319No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA60.12419788863589319No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT60.12419788863589319No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA50.10349824052991098No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC50.10349824052991098No Hit
CTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAAC50.10349824052991098No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG50.10349824052991098No Hit
GGAGAAGGTGGCAGTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCT50.10349824052991098No Hit
GTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGC50.10349824052991098No Hit
GCATGGGGGTACCCCAATCTGAAGTCAGTAAATGAACTAATCTACAAGCG50.10349824052991098No Hit
CACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCA50.10349824052991098No Hit
CACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAAC50.10349824052991098No Hit
GCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGA50.10349824052991098No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT50.10349824052991098No Hit
CCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCACCACCAC50.10349824052991098No Hit
CTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGC50.10349824052991098No Hit
CCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAA50.10349824052991098No Hit
GACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGGAAGTT50.10349824052991098No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC50.10349824052991098No Hit
GTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACAC50.10349824052991098No Hit
GACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCA50.10349824052991098No Hit
CCACTGACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACAC50.10349824052991098No Hit
TCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACC50.10349824052991098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)