FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4831
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG270.5588904988615193No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT260.5381908507555372No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA240.49679155454357277No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC230.47609190643759053No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT220.4553922583316084No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG200.4139929621196439No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG190.3932933140136618No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT170.3518940178016974No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG170.3518940178016974No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT150.31049472158973296No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA150.31049472158973296No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA150.31049472158973296No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG150.31049472158973296No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT150.31049472158973296No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT140.2897950734837508No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA140.2897950734837508No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC140.2897950734837508No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA130.2690954253777686No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA130.2690954253777686No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC120.24839577727178638No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA120.24839577727178638No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG120.24839577727178638No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG120.24839577727178638No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC120.24839577727178638No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA110.2276961291658042No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA110.2276961291658042No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG110.2276961291658042No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG110.2276961291658042No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG110.2276961291658042No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA100.20699648105982196No Hit
GCTGGTAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCA100.20699648105982196No Hit
GCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGTG100.20699648105982196No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA100.20699648105982196No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA100.20699648105982196No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG90.18629683295383978No Hit
GTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACAC90.18629683295383978No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC90.18629683295383978No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG90.18629683295383978No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA90.18629683295383978No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG80.16559718484785757No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGGTCAGTGG80.16559718484785757No Hit
GCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACCCG80.16559718484785757No Hit
CTCTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCA80.16559718484785757No Hit
CAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGCTCACTCAGG80.16559718484785757No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA80.16559718484785757No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC80.16559718484785757No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT80.16559718484785757No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG80.16559718484785757No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT80.16559718484785757No Hit
TCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACC80.16559718484785757No Hit
GGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGG70.1448975367418754No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT70.1448975367418754No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC70.1448975367418754No Hit
GCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCAC70.1448975367418754No Hit
GCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCT70.1448975367418754No Hit
CACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCA70.1448975367418754No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC70.1448975367418754No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC70.1448975367418754No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG70.1448975367418754No Hit
TGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACAC70.1448975367418754No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC70.1448975367418754No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC70.1448975367418754No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC70.1448975367418754No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC70.1448975367418754No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA70.1448975367418754No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT60.12419788863589319No Hit
CTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAG60.12419788863589319No Hit
GGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGG60.12419788863589319No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC60.12419788863589319No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC60.12419788863589319No Hit
CTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAAC60.12419788863589319No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG60.12419788863589319No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG60.12419788863589319No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC60.12419788863589319No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC60.12419788863589319No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC60.12419788863589319No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC60.12419788863589319No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG60.12419788863589319No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT60.12419788863589319No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG60.12419788863589319No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC60.12419788863589319No Hit
TCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAG60.12419788863589319No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA60.12419788863589319No Hit
CCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCG60.12419788863589319No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC60.12419788863589319No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT60.12419788863589319No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG60.12419788863589319No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT60.12419788863589319No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT60.12419788863589319No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA60.12419788863589319No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT60.12419788863589319No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT60.12419788863589319No Hit
CAATCAGGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGC60.12419788863589319No Hit
CCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACT50.10349824052991098No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA50.10349824052991098No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG50.10349824052991098No Hit
CTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGG50.10349824052991098No Hit
GGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCT50.10349824052991098No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA50.10349824052991098No Hit
TTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCG50.10349824052991098No Hit
TTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGG50.10349824052991098No Hit
TCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTAC50.10349824052991098No Hit
GGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGC50.10349824052991098No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT50.10349824052991098No Hit
CCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTT50.10349824052991098No Hit
CTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGC50.10349824052991098No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT50.10349824052991098No Hit
GAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGC50.10349824052991098No Hit
ACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAA50.10349824052991098No Hit
TCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTG50.10349824052991098No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA50.10349824052991098No Hit
GGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTC50.10349824052991098No Hit
CTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTC50.10349824052991098No Hit
CATCTCCACCTTCTACAAAATGGGTGGTCTTTTTCTTCATTCCACCTCGT50.10349824052991098No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT50.10349824052991098No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT50.10349824052991098No Hit
GGCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGC50.10349824052991098No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT50.10349824052991098No Hit
GGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAG50.10349824052991098No Hit
CCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGA50.10349824052991098No Hit
ACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGG50.10349824052991098No Hit
GTATCAACGCAGAGTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGC50.10349824052991098No Hit
GGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACC50.10349824052991098No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT50.10349824052991098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)