FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT350.7197203372403865No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA250.5140859551717047No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT220.45239564055110015No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG180.3701418877236274No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG170.3495784495167592No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC170.3495784495167592No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT160.32901501130989097No Hit
GTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACAC160.32901501130989097No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA150.30845157310302285No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC150.30845157310302285No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT150.30845157310302285No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA140.28788813489615467No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC140.28788813489615467No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT140.28788813489615467No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT130.26732469668928643No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA120.24676125848241826No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT120.24676125848241826No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG120.24676125848241826No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA120.24676125848241826No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT120.24676125848241826No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT120.24676125848241826No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG110.22619782027555008No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA110.22619782027555008No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA110.22619782027555008No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA110.22619782027555008No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC100.20563438206868187No Hit
CACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCA100.20563438206868187No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC100.20563438206868187No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG100.20563438206868187No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG100.20563438206868187No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG100.20563438206868187No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA100.20563438206868187No Hit
AGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCC100.20563438206868187No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC100.20563438206868187No Hit
CCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGC90.1850709438618137No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC90.1850709438618137No Hit
CCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCACCACCAC90.1850709438618137No Hit
AGCTGCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTC90.1850709438618137No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA90.1850709438618137No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG90.1850709438618137No Hit
TGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACAC90.1850709438618137No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC90.1850709438618137No Hit
CGGCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCT90.1850709438618137No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA90.1850709438618137No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC80.16450750565494549No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG80.16450750565494549No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT80.16450750565494549No Hit
GTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGG80.16450750565494549No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT80.16450750565494549No Hit
GGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACC80.16450750565494549No Hit
GCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCT80.16450750565494549No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT80.16450750565494549No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT80.16450750565494549No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT70.14394406744807733No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA70.14394406744807733No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC70.14394406744807733No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG70.14394406744807733No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCAC70.14394406744807733No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA70.14394406744807733No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG70.14394406744807733No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG70.14394406744807733No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG70.14394406744807733No Hit
TTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTA70.14394406744807733No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT70.14394406744807733No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG70.14394406744807733No Hit
ACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACT70.14394406744807733No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT70.14394406744807733No Hit
CCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCA70.14394406744807733No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC70.14394406744807733No Hit
ATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAG70.14394406744807733No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG70.14394406744807733No Hit
CCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCC70.14394406744807733No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA70.14394406744807733No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA60.12338062924120913No Hit
CCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCC60.12338062924120913No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT60.12338062924120913No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG60.12338062924120913No Hit
CCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAG60.12338062924120913No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA60.12338062924120913No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA60.12338062924120913No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA60.12338062924120913No Hit
CTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTG60.12338062924120913No Hit
CCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCA60.12338062924120913No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC60.12338062924120913No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC60.12338062924120913No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC60.12338062924120913No Hit
CCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTT60.12338062924120913No Hit
ACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTT60.12338062924120913No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG60.12338062924120913No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC60.12338062924120913No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC60.12338062924120913No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC60.12338062924120913No Hit
CACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAG60.12338062924120913No Hit
TCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGC60.12338062924120913No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG60.12338062924120913No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT60.12338062924120913No Hit
TAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGT50.10281719103434094No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG50.10281719103434094No Hit
ACTACATCTAGGACGCCTCCGGGTCAGTGGAAGCCCCAACGGGAAAGGAA50.10281719103434094No Hit
GTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTC50.10281719103434094No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA50.10281719103434094No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC50.10281719103434094No Hit
GTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGC50.10281719103434094No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA50.10281719103434094No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG50.10281719103434094No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA50.10281719103434094No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA50.10281719103434094No Hit
GTATCAACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCA50.10281719103434094No Hit
CCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTG50.10281719103434094No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT50.10281719103434094No Hit
TCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAG50.10281719103434094No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC50.10281719103434094No Hit
CCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAA50.10281719103434094No Hit
GGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCT50.10281719103434094No Hit
GGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCT50.10281719103434094No Hit
CATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTG50.10281719103434094No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG50.10281719103434094No Hit
CGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCA50.10281719103434094No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG50.10281719103434094No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT50.10281719103434094No Hit
GCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGG50.10281719103434094No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT50.10281719103434094No Hit
GTATCAACGCAGAGTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGC50.10281719103434094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)