FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCTGCTCCACCAGGAAGATCGTCAGTCCTTGCTTGTTGATCTCGCGC26.25No Hit
GCGTGGGCAAGACCACGCTCGCAACGCACATCGCCGGCGAGCTGGCGATG26.25No Hit
GCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCC26.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGT13.125No Hit
GNAGAGACCTTGCATCTCCCGGCTGCCGATCGATCAATTCTGAGCGGTGC13.125No Hit
GCGCAGGCTGCACGCCGGGCGGCGGCCACAAGGCCGCCGCCCAGCGTGCA13.125No Hit
CTTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGG13.125No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG13.125No Hit
CACCGAGATACACTCTTTCCCAGCAACTCCAGTCCTTGCACTGTCTCTTA13.125No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGGCACCGA13.125Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTAGTTACGAGTCGGCGCACGCGGCCTCGGTCCGGTTGACTTTGCGG13.125No Hit
CGAGGATCGGGATCCGCTCGCCCCAGCGCGACGGGTTGTAGGACCAGCCG13.125No Hit
GGTCGGAAGCGCACCTTTTGCCTCCGGACAAACTTTTCCCACGCCTGCTT13.125No Hit
CCACCTTCTCCATCGTCTCCACTGGCCTCGTGGTGTATGACTACCAGCAG13.125No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCTGTCTCTT13.125Clontech SMART CDS Primer II A (96% over 26bp)
ATCATGCGCATCCACCGCGAGCACGGCGTGCACATCAACAACCCGCACGT13.125No Hit
CTCGCCTACTGACGGCGGGGGAAAGCAACGAGAGGGGGAAGACTGAGCTC13.125No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA13.125No Hit
ATACAACACTATGCTGAAAAACCACAACAGCTTGGTTAAGCGGAGGAGAG13.125No Hit
TTCAGAGACAGGGAACCGTCATCCAGCTGCCTATGAAAATGACAAATGAG13.125No Hit
GCCAGGTTGGGGGTGATCTTGTTGAAGGTTGGGTGATCCTGATCATGGTG13.125No Hit
TATCAACGCAGAGTACATGGGGGAATGGGAGAGGCGGGCCCAAGGAGGGA13.125No Hit
ACGCAGAGTACATGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGG13.125No Hit
GGGCGGTATCGCAGGAGCGCCGCAAATCCGTCAATTCGCGCTCGAGCTCG13.125No Hit
CATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAA13.125No Hit
AGTCAGACCACGATCGTGCGGCACCACGCATAGATGCTGCGCGGCTGCCG13.125No Hit
GAGCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCT13.125No Hit
TGATGATCGTCTCGGTGCAAAGCGAGAACGTTTCCGGATCCTCCTTGATT13.125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)