FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATAGCTGGCGATGGTCTGGCTGGCATGAATGGCGAGAATGCCCCGT36.382978723404255No Hit
GGCCCAGCATGGCACAGCAGGGAGGCCACTCTGTTTCTTCAAGTATTCTT24.25531914893617No Hit
GGCCAGGAGCGCCTGCCAGTCGCCGTGGTTGCGCCCGTCGGCCAGGCCGA24.25531914893617No Hit
GTACACTGCAGGAGAGTGCCTGGCAAAAAGATCAAATGGGGCTGGGACTT24.25531914893617No Hit
TGATGGAATTCTACTCGGGATAACGCTGGTTTCAGCCGTTTTGGCGATGG24.25531914893617No Hit
TCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC24.25531914893617No Hit
GTAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGC12.127659574468085No Hit
CAGGGAGGACAGAAACCTCCCGTGGAGCCGAAGGGCAAAAGCTCGCTTGA12.127659574468085No Hit
CATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCC12.127659574468085No Hit
GCTCTTGAAGCTGCTCACTGGGCCTCGTTGAAGCGGGTCACCATTGTCTT12.127659574468085No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA12.127659574468085No Hit
CGTCAGCGTCAGGCCGAGGCCGAGGCCTTCGAGCGCACCAAGAAGGCCGA12.127659574468085No Hit
ACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACT12.127659574468085No Hit
GCCACGCAGCTGCGCGACGACGCAGCGCGCATTCTTCAACCTCTGAACGA12.127659574468085No Hit
TCTTCGGCGTGTAGGCGAGGGCGGTCGAGCCGTCCGCCAGCACCGAGGGG12.127659574468085No Hit
CTTCCACGTCATCCGCATCAACAAGATGTTGTCCTGTGCTGGGGCTGACA12.127659574468085No Hit
GTCCTGCCATACGTTGCTTCTCTTGCATAGAGTCTGCCATGACCCGAACT12.127659574468085No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA12.127659574468085No Hit
CTTTAATACTGTGAAGGAGGCCAAAGAACAGACAGGAGCAACGGCTTCTG12.127659574468085No Hit
GCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAG12.127659574468085No Hit
CCCATAATCACTGACCAGCCTTGACACTCCCCTTGCAAACCATCCCAGCA12.127659574468085No Hit
GGTGCCTTAGCAGCAGCCATTGCAGTCTTTTTAGATGCTTGCTTAGCCTT12.127659574468085No Hit
TGGTAGATCCGTGCTCCGACAAATAGTCTGAAGTGCAGGATGGCTGTAGA12.127659574468085No Hit
TTATCGTCGCGGTTACCGTAATACATCAGATCAGTTAAGTAGGGCCATAT12.127659574468085No Hit
CCTCCTGGCCGTGCCAGCCCCACTTCCTGCTCAAGTTCCGGTTGCAGTAC12.127659574468085No Hit
TCCCTGTGCGGCATTCGAGCTCGCATAGCCGAAGTGCCGCGCGATGGCTG12.127659574468085No Hit
ACCCGGCCCAGGGCTTTCTGTGGCTGGTCGAGACGGATGAGGGCTGGGAG12.127659574468085No Hit
GTAGGGTTGTCTTTTCATCCATATCGTCCCTGGAATTAACTGTCCTGCTA12.127659574468085No Hit
ATCATCAGTACAATGAATAAACGGCTGAGTGAAGTCACGAGTGATGAAAT12.127659574468085No Hit
GTCCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCTGTTAAGAAGCCCCGGACAGTTAACGTGGAAGATTCCTGG12.127659574468085No Hit
GGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCT12.127659574468085No Hit
GGTCGATCGCCACCCCGCCAAGTCCCGAGCCCGCCGCGATGCCCAGGTAG12.127659574468085No Hit
CCACTGATATCCTCCTTTGGGGAAAGGCTTGGCACACAGCAGGCTTTCAC12.127659574468085No Hit
CATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCATGCC12.127659574468085No Hit
CTCGTTGAGCAGTTTCTGATCCTGATCGTCGCTGGAGCAATTGATGGCGG12.127659574468085No Hit
AGGTAGTTGGTCAGCGCCATGCGGCCGGCCGGTGCGAGCATGGCTTCGAG12.127659574468085No Hit
CGTCAGATGTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACT12.127659574468085No Hit
GTCCACCACTAAAATGCCTCTGCCACTTTGAATTCTGTGCTAATTTTGTG12.127659574468085No Hit
GGGGCTCCTGGAAAGACAGCAGGCTTCCTGCTGGGCGTTCCCTTGTTGGA12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)