FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCAACGTTTGCGCGCGCCCGTTCTGCCGAGCGGATGCTATCGGAACT36.382978723404255No Hit
ATATAGTGCTTTTGTGCCGATAGAAAAATCACTCCTTCTGGGGAAAGGCA24.25531914893617No Hit
GGTGGTGCAGATGACCAACGACGACATCAAGAGGATTGGGGTGCGGCTGC24.25531914893617No Hit
GGGGAGAGGGAACAGTTTCCGGAAACGCCGAGACGATCGATGACAGACAC24.25531914893617No Hit
GAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCT24.25531914893617No Hit
ATACGGCTGTCGATGATGAAATCGGCGATCTTCATGGTGATCAGCGAAAC24.25531914893617No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG24.25531914893617No Hit
CAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATG12.127659574468085No Hit
GTTCCACAGCATGGATGTGGTGCCAACGAAGCGGTGCGAGGCGCTGCTCT12.127659574468085No Hit
CAATTAGCCTTGTCTTTTCCTGGCGCAGCAGTTTGTGCTTGACTCGTACC12.127659574468085No Hit
GTACTTCACCTTGACTGGTCACGTAGAGACTGACTGGAAGATTGAACATT12.127659574468085No Hit
GGCCTTCTTTTCGAGGCCCTCGGCCTCGGCCGTCAGCTTCGCGCCAATCG12.127659574468085No Hit
ATTCAGGAAGTGCTGGAGCAGCGCCACGATCTGGTGGTCAAGGCCACCCA12.127659574468085No Hit
GTTTTAGAACAAGGCACAATACACTTGAAAATCTATTGCACTTTAGGAAA12.127659574468085No Hit
GCCTACGGCACCGCTGCGCAGCCGCCCGGCGGCGCCAACTTCCAGCTGGC12.127659574468085No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC12.127659574468085No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC12.127659574468085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAA12.127659574468085No Hit
CTGTGTAGCATTTGGCAAAGGAGAAAATGCCAAGAAGTATCTTCGAACAG12.127659574468085No Hit
GATGGCCGGCGCGGGGCGGGTGATCCGCCTGAGCACCATGCCGACGATGC12.127659574468085No Hit
GTGTGTAGCTGGCCCCATGCTGGTATCTCCTTTGGTATGGTCCCAGTGCT12.127659574468085No Hit
CACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATGCTTG12.127659574468085No Hit
GACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGA12.127659574468085No Hit
TCACCGGGCTGGTGCAGGCGCCGACGGCGCTGGCGATGTTCTTCGCGGGC12.127659574468085No Hit
TGACGCGCCAGTTGAACGCGCCCAGCGCCAGATCCACTCGCGACGTGTAG12.127659574468085No Hit
CCTGGATCCTGTTGACACCCCAAACCCAACAAGGAGGAAGCCTGGGAAGT12.127659574468085No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA12.127659574468085No Hit
GGCCTGGGCCGCAAGAAGCAGGGCATTGTAACGCCTATCGAGGCCCAAAC12.127659574468085No Hit
GTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGA12.127659574468085No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC12.127659574468085No Hit
GTCCTCTACAGAAGGAAGCACAAAAAGGGACAGTCGGAAGAAATTCAAAA12.127659574468085No Hit
GTCTTCAAATTCATCAGCATTGAACTTGGTGAAGCCCCACTTCTTTGAGA12.127659574468085No Hit
GTACATGGGGGAGGCTCTTTCCAACGCTGTAAAAAAGGACAGAGGCTGTT12.127659574468085No Hit
CATAAGAGCCTAGGCCAGACCGCCCGCTCCGTTGAAGTCTTGTGATTGGA12.127659574468085No Hit
GCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCA12.127659574468085No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCTGT12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAAGTCACAGAGCATGGCCCTGGTGGGCGGCGAGGGGTGCGAGTGGA12.127659574468085No Hit
CTGGTGGTTTCAGGAAGGGATGATCCACCAACACATTCTCAGCCAGACAG12.127659574468085No Hit
CGCTGATGCTGGTGCTGATCCTGGGCGGGCTGTCGTTCGCCGCACAGATG12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)