FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGATCTGCTCGGTGAAGTGGTCGGCGATCTCCGCCCGCTGCCGCGC411.11111111111111No Hit
GCGAGGGAGTGAATCTGACGCACGCGGTACCAGCCCTGTCTCTTATACAC25.555555555555555No Hit
TCTCGTCGATGTGGGCCTGGCCATCGGCGCCGGGCATGTTGAGGTCGATC25.555555555555555No Hit
CGACCAGCGAGTTCACGCCCTCGCACAGCTCCGCGATCGAACCCTGCTTG25.555555555555555No Hit
ACCAGTGGTAGCGACACTGGATTCCACGCCGCCGTCCGGGGATGCCAGAC12.7777777777777777No Hit
ACTAACCTCACCCCAGTCAGGCTGGGCCCATCTAGCCTGAGAGAAGCCTA12.7777777777777777No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAGTA12.7777777777777777No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAG12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAC12.7777777777777777No Hit
GTCATACCGCGCTGTGCAACTGGCGCAACGTAGCTGGGCGCGTCTCGAAG12.7777777777777777No Hit
GGTCGACTGCATGGTATCGACAGCGCGATCGAGATGACCCGACTCTTCCA12.7777777777777777No Hit
CGCGAGGACTTTGGGGTGAAGATCGTCGGGTTGGCCGACACGGTCCGGAC12.7777777777777777No Hit
TCTCGGGGCGCCGCATCCCATGTACTCTGCGTTGATACTGTCTCTTATAC12.7777777777777777No Hit
CATCAAGGCCGGGTCTGTTCGCATCGAGACGGCTGACGGCGGCGAAGAAT12.7777777777777777No Hit
ACGGTGGCTTCATCGTCAGCGAATCGAACGGTCACCGCTCGCGCGACGCC12.7777777777777777No Hit
GGGTGAAGTCGACCCGCCGCACGCCGTGGCGGGGATCGGTGGAGCCGAAG12.7777777777777777No Hit
ATCTTGCTTCGTGACTGGAGTTCAGACGTGTGCTCTTCCTGTCTCTTATA12.7777777777777777TruSeq Adapter, Index 6 (96% over 31bp)
GTGCCACAGCCCGCCCATCATCGAGAAGGTGGCGTAGGTTCCGGAAGGGC12.7777777777777777No Hit
AGTCGGCGCTGATCGTTGGCGTCTGAACCAGCCGTTCGGCGATGCGATGG12.7777777777777777No Hit
GGACGTCACCAAGGGGTCAACCAAGAGTATCCAGCTAATGTTGTTTACTA12.7777777777777777No Hit
CCTCCGGATTGGGCATCGGACCGGACCTGTTGATCATGGTGGCAGCCGCT12.7777777777777777No Hit
GCCCTTGGCAAAGGCTTCGGCCTGAGCCAGCAGCGTCGTCTTGCCGTTGA12.7777777777777777No Hit
GGCTTCCCTTTGTGGGTAACCCGACCTTCCTCTCTGGCTGCCCTTAACAT12.7777777777777777No Hit
AACGTGAAACGAGCTTTGAGTTGTACATTGGGTCAGGCAAAATTTCCTGA12.7777777777777777No Hit
TATTGCTAAACCGCGACTAACCGGTAGGGAATTTCATGATGTGCGAAAAA12.7777777777777777No Hit
GTTTCAACATGAGTGGTTTATGGACTACACTTTTAAAAACCCTCCTCTAC12.7777777777777777No Hit
GCTGTTAGCTCTTCTGATAGTGTCTCTTCCAGCACAGCCATAACCGCATC12.7777777777777777No Hit
GTCCACACCTTGGGCGATGCCCACGTGTACCTCAATCACCTCGACCAGGT12.7777777777777777No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
GGACACACATTAACACCAGTAGAACAAGATTACTTAGAAGCTGTCTCTTA12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)