FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGCCGGCATGTTCGCCGCCACCGACCCGGCCCTGGCCTACGCGGTGG411.11111111111111No Hit
ACCTCAACGGGAAGCCGTTCAAGGTCGTCAAGTACGCGACCGACATCACC25.555555555555555No Hit
GGCTGGTACCGCGTGCGTCAGATTCACTCCCTCGCCTGTCTCTTATACAC25.555555555555555No Hit
CTTGGGGTCTATGCCGTTGGTGGGGCCGCCGGCCTTGCTGCGCGGGGATT25.555555555555555No Hit
GAGTACTTTTTTTTTTTTTTGCGTTGATACCACCCATGTACTCTGCGTTG12.7777777777777777No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
CACCGGTGACAGTGATGGTGAAGCCGTCGCCGGCCACCATCGGCGTGCCG12.7777777777777777No Hit
GGGCTGACCTCCTCCTTGGAAGCAGGATGGCTGGGGCAGTTCTGTGTATC12.7777777777777777No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGCACACGTCTGAACTCCAGTCACGAAGCAAGATCTGTCTCTTATA12.7777777777777777TruSeq Adapter, Index 7 (96% over 30bp)
TATCAACGCAGAGTACATGGGATGCGGCGCCCCGAGACTGTCTCTTATAC12.7777777777777777No Hit
AGGTTATACTAAGCGTCAGGAAATTTTGCCTGACCCAATTTACAACTCAA12.7777777777777777No Hit
TCCATGTAGTAAACAACATTAGCTGGATACTCTTGGTTGACCCCTTGGTG12.7777777777777777No Hit
CGGATAAAGCACTTCGATCACCATTGGGCGCGTCTCACTTCGAGACGCGC12.7777777777777777No Hit
CTTCTAAGTAATCTTGTTCTACTGGTGTTAATGTGTGTCCCTGTCTCTTA12.7777777777777777No Hit
CGCTCACGGTGACGGTGCGGCAGGGCGAAGCCATGGGCTCGCCTTCCGCC12.7777777777777777No Hit
CAGCACGACGGCTGCCCCCAGAGCGGCTGCCACCATGATCAACAGGTCCG12.7777777777777777No Hit
TTGTGTGCCTGCACACCCGGGGCTAAGAGCATGATAATCAACGCAATAAT12.7777777777777777No Hit
TATCCAGGTAACTAGGTAGAGGAGGGTTTTTAAAAGTGTAGTCCATAAAC12.7777777777777777No Hit
GCCGGATACGGGTCATAACCAGTCAGGGTGATATCGGACAGCTCGAACCC12.7777777777777777No Hit
GATCAAGCAAGATCGGCAAGAAAAGCTGGCGGAGCAAAGAGCGCAACTGA12.7777777777777777No Hit
GCAGTGTGAAGGTCATCACCGATGAATGGGAAGTTCGATGATCCTCTCGA12.7777777777777777No Hit
CTGCTTCTGATCGCTACCCGCTTCGATGACGGCCATAACCTGACCGCCAT12.7777777777777777No Hit
ACTCACGAGTCATGAGGAGGGCTTGGGACAGCAGCATTCCGCGTCGCTGA12.7777777777777777No Hit
GGACAGCACGGTCACGCAGTTGGGCTGCACTTCCAGGATGCCACCGGCCA12.7777777777777777No Hit
GAGTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAAAAAAAAAAAAA12.7777777777777777No Hit
GTCAGGGGCATGTCGCCACCCGGTCGATTTCAGGACTTCCCATGGACACC12.7777777777777777No Hit
CAAGAACGCGATAAATTGCTGCCGGCCATGGCGGATGATGTTGCTTCCCT12.7777777777777777No Hit
CTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATTCACCAAAG12.7777777777777777No Hit
ATTTGCTGGTAACCCGATCGGGAGGGTCGGCGGGCCGACGGGCCGAGCTC12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)