FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAA33.79746835443038No Hit
AGCCAGTTTCTTGGCGTGGCGATTGGCGGTTCGCTGGGCGGCTGGATTGA33.79746835443038No Hit
GTCCCGGGAGGGCGGCCGTACCCACCGCGCCGCCCAGGCGCTGGCCGGTC33.79746835443038No Hit
GAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGG22.5316455696202533No Hit
GAACTCGGTGATGAGCGCCGTCACGTCGTCGGGCCGCTCGAGGTTGGGCA22.5316455696202533No Hit
ATACTATGGAGAATTATTGAGTGAAAATACAAACATGAGTCGAAGTATTT22.5316455696202533No Hit
AACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGC22.5316455696202533No Hit
GTGGCTTCCTGGCCTGCCTCGTCGACTATGCCGAGGCCCACGCCGACGAG22.5316455696202533No Hit
GTGCTTAATGTGCAAGATCTTCTCATCTTTAGATCTGGCAAAAGCTTCCT22.5316455696202533No Hit
ACTCTATATTATCTACATGATCTGCGCATGTCGCTTATTTTTAATGAGTT22.5316455696202533No Hit
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTAT11.2658227848101267Clontech Universal Primer Mix Long (95% over 21bp)
GTCTAAGGTCTGCCTGATTGTTTTTGCTTTGTGAGTAACTTGCACTTTCT11.2658227848101267No Hit
GCCGTAACTATCAGGTTCTTCTCATTAAAAGCATGGCAAATTTCCAAATC11.2658227848101267No Hit
GCTTTCGGCGGCGCTGAGCCGGGGGTGGGTCGTCCCTGTCTCTTATACAC11.2658227848101267No Hit
GTTTCAAGCGATTCTCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGG11.2658227848101267No Hit
GTTCTCAGAAAGGCGGGCAGTCGCCTGGAGGAGTGGAGAGAAGGGAGGTC11.2658227848101267No Hit
CCATGAGCTAAGGACAGTAGCTGGCAGCCTTGTAGAGTGCATAGAGCCAC11.2658227848101267No Hit
CGTTCCGACGGCTCGCAGGCAACCGCCTCGGCTCAGGCCTTTCGTCGGCC11.2658227848101267No Hit
GCATCGACGCGGTGGCCCGCGCGCGCGGCCGCCGCCTGCTGCTGTCCAGC11.2658227848101267No Hit
CAGCAAGAGATCGCCCGCGCCGTTGATATTGACGGCCAGCGACGCGGCGT11.2658227848101267No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG11.2658227848101267No Hit
GGCTGTGAGAGAGTGCCATTGATGAAACGAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
CCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGAC11.2658227848101267No Hit
GATAAAATCATGCCCTGCTAGCTAGTATATGTTGGCTGATTAGACATTAC11.2658227848101267No Hit
CCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTAT11.2658227848101267No Hit
GGTAGATCGTGACGTCCGTGTCGGTCATGGTCGAGTAGGCGACCTTGGCG11.2658227848101267No Hit
CTCTCTGAGTAGGTGCCATTCCTGCAGCCCCATCATTTTATGATAACATC11.2658227848101267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11.2658227848101267No Hit
GGTCCAGCGCGACATCGACGGCGGCGGCGTCGGCTGCGGCGGTCTTCGCG11.2658227848101267No Hit
GTCCAGCACCGCGCGGAAGAATTCATCCGGCACCTGGGTGAGCAGGCCCG11.2658227848101267No Hit
GGGCGAAGAGCGCGAGACAGCGATTGGCTTAACCAAAGACAAACAGGGCG11.2658227848101267No Hit
AGTAATGTTACTCTCTAGCCTCTAGGGGAGCCATGCAGCAGGGATAGTAG11.2658227848101267No Hit
GCCACATCCTGCTCTCGCGGCGCGTGGCCGACAGCGGCCTGTACCCGGCC11.2658227848101267No Hit
GCTGGGAGGCCTTGGGAACCGGGCTCATGGCGCCGAGGTCGGCGCGCCAC11.2658227848101267No Hit
CTACATTGGGGTAGTGAAAGGTCAGAAACATGAACATCCACCTGAAAGGC11.2658227848101267No Hit
GCGTGAGCGCGGTGCGCACGCCGCCCCCGCCCGCCGGGCCGCAGGTGTAG11.2658227848101267No Hit
ATTCCATATGATGATGACTCCATTCGATGAAATTCGATGATGATTCCATT11.2658227848101267No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG11.2658227848101267No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG11.2658227848101267No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11.2658227848101267No Hit
CCTTTGCTGAGGGCGCTAACCGAGCCGTCAACCAGGTGCTGGTGCGGTCT11.2658227848101267No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTC11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC11.2658227848101267No Hit
CCCTTGACTCATCCTGTGGTCAACAGGGTCTTTAAAGGGGTTCTTGAGTT11.2658227848101267No Hit
AATCTATAGCGTACAGTCTATCAAAGCATGTGCGGTGAATTACAGCTTGA11.2658227848101267No Hit
GGGGTAGGTGGAGCCGGCCTGCTGCCGCGCCGCGTTCTGCTGCTGGCCTT11.2658227848101267No Hit
ACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCATG11.2658227848101267No Hit
ATGCATTTAAGTTACTTGGAAAAAATTTAATCCTTTCACATCTTGCTTTT11.2658227848101267No Hit
CAACAACGGCGTCCCCCAGCCGCACGCGAAGACCTGGCGCACGCTGGTGG11.2658227848101267No Hit
GAACCAAGCCGACCACGAAACCGGACGACCAGCCCTCTACGACAACGCTA11.2658227848101267No Hit
CTCCACCGGCGTCCCCCAGCCGCACGCGAAGACCTGGCGCACGCTGGTGG11.2658227848101267No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA11.2658227848101267No Hit
GACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG11.2658227848101267No Hit
TTTCTCTCCTTGGTGGGCAGGTGAGTTGTGCCTGATTATGGGCACAGGAC11.2658227848101267No Hit
GGATTCTGACGAAACCTGGTTCCCCGCCGCGATGGTTGGTGTCCATACTC11.2658227848101267No Hit
CATCTGTTTCTTGTCAGGGCATTGACTGATAATAGTTGTGTCTTTATAAT11.2658227848101267No Hit
GTGTCAAACTCTCCGACGAGGTGTTTAAATCCAATTTGGAGGGTGTTGGG11.2658227848101267No Hit
CCATTGCCAACGGGCGGTAGCGCTTCGCAAATCGTGCGGTGGTGGCAGTC11.2658227848101267No Hit
CGTCCGCGAGCGTGCCGAACCGCGGGTCCACGGCGCGGTAGTCCGCGACT11.2658227848101267No Hit
GAGCTTACCGGCGTCGATCCGTCGAAGCTCGACGCCCTGATCGTGAGCCA11.2658227848101267No Hit
ACATGGATGCTACCAACCACTGTTGTTCGTCTTCATTGCTGTCGAGTATT11.2658227848101267No Hit
AGCTTGGTCACGTGCGCGGCTCGATGGGATTGGGCACCATCCTCACGAGC11.2658227848101267No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGCT11.2658227848101267No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG11.2658227848101267No Hit
GTTCAGGATGTCGGAAGAGCGAGATGGCCGACGCAGCTAGTCCGCCCCAC11.2658227848101267No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)