FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003676556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATACGGCGGTTCTTTCATGGTACTGGCGACTGCCAGCCACACTGCGG33.79746835443038No Hit
CTTCTTCCAGAACGGCCTCGGCTACGAGCCGCTGCGCTCCGGCCTCGCGG33.79746835443038No Hit
TCTGCAACCAGGCAGTGGTAAAAGGGGTGCTCTCCAAAACCATCTTCTAG33.79746835443038No Hit
CGCCTTAATGGTCTCGCGTCGATGCCTCAGTTCCTCTAGGGTGCGCGACC22.5316455696202533No Hit
GTATGGTTGGGCTTGCGTGGTGGCTGTCAGAAAGTGTGGGGGTACAGGTG22.5316455696202533No Hit
CGTTCCAGGTCCAGCTCGGCGCCGGGGAGGACATCCACCAGGCGGCGGGC22.5316455696202533No Hit
GTTTCTGGGTGGAGCCTATTTTTCCAGCTTTTGGCTGCTCTGAGCCGAAG22.5316455696202533No Hit
CATTATACCATTCTATCAAGAGTATAATAAAATACTTCGACTCATGTTTG22.5316455696202533No Hit
GCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGA22.5316455696202533No Hit
GTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCA22.5316455696202533No Hit
TGCGACGAGGTTCGCGAGGCTGCCTGCGGGCATGACACCCGATGCGGTCG11.2658227848101267No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11.2658227848101267No Hit
AACCAGTGGTCACCAGAGCGAGCGGAATCTTCACCGAGGCCAGCCACTAC11.2658227848101267No Hit
GATCCAGCACCGGTAAAAATAGACCGGCACGCGATTTTTCGCCGCCATTT11.2658227848101267No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.2658227848101267No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAA11.2658227848101267No Hit
GCGCTCACGTATGTTCTTCTCTTTTGGGTTACTTTCTGGATTTGACATTG11.2658227848101267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCCACGATGAT11.2658227848101267No Hit
GAACGGCGAAACCCGTGCCGACGCGGCCGCGAAGGACGGCTCCACGGCCG11.2658227848101267No Hit
CTGCTCGTCCGGATCGCCGTGGTAGCTGGACAGCAGCAGGCGGCGGCCGC11.2658227848101267No Hit
AATTTGACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATT11.2658227848101267No Hit
GGTACACCGTGCCGTGACTTAAATTGCCCCAGACGGTGGCTCTATGCACT11.2658227848101267No Hit
AACCTGCGCGGGGCAGGGAACATCACCATCAACGGCCTTAACGCCGCGTC11.2658227848101267No Hit
ACACCATCGCACCCGAAGTCGGCAGCGACAAATGGGCCCCGGCCCTGGGC11.2658227848101267No Hit
CGCCTCCGGCTTGAGCAGGTCCGATGCCAGTTCCTGCGTCAGGGCGGCCA11.2658227848101267No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTT11.2658227848101267No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG11.2658227848101267No Hit
GTACTGAATCCTGCAGGCAATTGTAATATAAATCACTAGGTGACAGAAAT11.2658227848101267No Hit
CCTTGGAGACAGGCCTTTCAGGTGGATGTTCATGTTTCTGACCTTGCACT11.2658227848101267No Hit
GGACGACCCACCCCCGGCTCAGCGCCGCCGAAAGCCTGTCTCTTATACAC11.2658227848101267No Hit
GTACCGAAGGTCGCAGGTTCGAATCCTGTCGGGGACGCGTTCGTCATGAG11.2658227848101267No Hit
GGCCACGCGCTACGTGCTGCCCGTGCTGGCACCCGATGGCGCCACGGGCA11.2658227848101267No Hit
GTATATAAAGCTATGATTACTCATTTGCGGCTTACTACCAGCTAAAAAGT11.2658227848101267No Hit
ATTATAAAGACACAACTATTATCAGTCAATGCCCTGACAAGAAACAGATG11.2658227848101267No Hit
AGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCA11.2658227848101267No Hit
GGTTGGAGTAGTGAGGTGAGAAAATGAAGGAAATGAAGTTGCCAGCTATT11.2658227848101267No Hit
TCGCGGACTACCGCGCCGTGGACCCGCGGTTCGGCACGCTCGCGGACGCT11.2658227848101267No Hit
TTTATATTCAGAACTCTTTTCATCTCGCACAACTGAAGTTGTACCTATTA11.2658227848101267No Hit
CTTGAGAATCTAGGTTGAACATGCTCCCCAGGTGATTCTGCAGCCCCACC11.2658227848101267No Hit
GTGGTGGCACACGCCTGTAATCCCAGGACTTTGGGAGGCCAAGATGGGTG11.2658227848101267No Hit
AAGGCGTGGTCACCAGCGCGCGCGGACGCGGCACCGAGGCCAGCGTCTTC11.2658227848101267No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11.2658227848101267No Hit
GTCATGAGCACTTCAGCGATCATCATGATGATCATTGCGATGGTCACGGT11.2658227848101267No Hit
CAGCTCTGCGCCTGAGCGAGGTAGTTCTCAAGTACCGGGTCGCGCTTCAT11.2658227848101267No Hit
TGTGGCCCGGCAAATGCGTGGGGATGGCTTCATCCACATCCTGCAGGAAG11.2658227848101267No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAACTGTCTCTTAT11.2658227848101267Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTA11.2658227848101267No Hit
GTCAACGTGTGCCATGAAGGCGGCTGGCTGGCCGAGGGCGAGATCTCCTA11.2658227848101267No Hit
CAGGATAAAGAGATCACAATCTAGACATGGTTCGGAGTTCCCCCTCATAC11.2658227848101267No Hit
CCAACGGCCACTGCCTGGAGACCGGCTTCCCGGAGCCCGGCGAGGTCATC11.2658227848101267No Hit
GATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCG11.2658227848101267No Hit
GCTTTAATCTTGGGCAATAATGGTGATTCACGTTTTGATTCCATTTACGG11.2658227848101267No Hit
TCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATC11.2658227848101267No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT11.2658227848101267No Hit
CACGCGCAGTGTGCTCTTTCATGGCTGGCTCGTGCTCACGCAATTGGGTA11.2658227848101267No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGCT11.2658227848101267No Hit
CGCGTGGCCGGCGATCACGGTCGGCGTCTCGCACAGCACCGTGGTGACGC11.2658227848101267No Hit
CGCGTGAAGATCGCCGGCCGCGTGTTCAGAGGAATCGTGAAAACAACAGC11.2658227848101267No Hit
ATTCTAGACACGATGGTGGGAAAAGAAAAAAATGAAATAAAGGATAGTGT11.2658227848101267No Hit
GAACGGAATCGCCATGGAATGTAATTGAATAGAATCTTCTAACGGACTCA11.2658227848101267No Hit
CTCTCAGGGGGAAGGACTCAGCTATGACTCAGAACCAACCGGCAGTTCCC11.2658227848101267No Hit
GCTCAGTAATATTCCATCGCATGCAAAAACCACAGTTTTTTTTTTTGACA11.2658227848101267No Hit
GCGCCCTACCGGGTCAAGGTTGGTTGGGGGCGTGCTTCTGCCGTTCGGCG11.2658227848101267No Hit
ATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTT11.2658227848101267No Hit
GCCTGGCTGTGCGGGAGATCTGCGGCCTTGGCAACGTCCTGGCCGAGGAA11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)