FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003676780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTTCGTGCACGTGGCCGTGGCCGGTGGCCTCTGTGCCGTGGCTGTGT211.11111111111111No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA211.11111111111111Clontech Universal Primer Mix Long (96% over 25bp)
GGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA15.555555555555555No Hit
ATGTTTATTTCATTCACTACTTTCGGATTTTCCTTTTGTATTTTAAGATT15.555555555555555No Hit
CACTTCAGTGATGAGAAAAGAGGCAATGGAATTCAACATAATCAAAGAAA15.555555555555555No Hit
TTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTAT15.555555555555555No Hit
GCCAAATATCATGACCACGGCAATGCCGATGCCCACTGCGCCGATGATGT15.555555555555555No Hit
CTATCACATTTTACTCATCAAGTCTCTTTCATTTCGGCAGTTTAAAGACA15.555555555555555No Hit
GGCCGGGCCGTGGAAAAAACACGCACAGTCCGGTTGAAGCCACAGAAGAG15.555555555555555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGCA15.555555555555555No Hit
GTGGAAGAGATGTCCATTGGAGAAATGGCTGGTAGTTACTCTTTTTTCCC15.555555555555555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAATGC15.555555555555555No Hit
CAGTAGAGCACTGGGATCACCACTAGGCCTAGGGGGCATATCAAGGGTTT15.555555555555555No Hit
TCGTGTATATGATGAAGAAGTGGAGGAGCCAGTACTCAAGGCTGAGGCAG15.555555555555555No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC15.555555555555555No Hit
CTATAACATTTTACTCATCAAGTCTCTTTCATTTAGGCAGTTTAAAGACA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)