FastQCFastQC Report
Tue 10 May 2022
EGAF00003676826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6820
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG290.4252199413489736No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT280.41055718475073316No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT260.3812316715542522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.36656891495601174No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA220.3225806451612903No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT210.30791788856304986No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC210.30791788856304986No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT180.26392961876832843No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT180.26392961876832843No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT170.249266862170088No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG160.23460410557184752No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC140.20527859237536658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.20527859237536658No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA130.1906158357771261No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC130.1906158357771261No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC120.17595307917888564No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC120.17595307917888564No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT120.17595307917888564No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT110.16129032258064516No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC100.1466275659824047No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC100.1466275659824047No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA90.13196480938416422No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC90.13196480938416422No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC90.13196480938416422No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT90.13196480938416422No Hit
TCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCT90.13196480938416422No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA90.13196480938416422No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT90.13196480938416422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.13196480938416422No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG90.13196480938416422No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG90.13196480938416422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.13196480938416422No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT80.11730205278592376No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC80.11730205278592376No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA80.11730205278592376No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA80.11730205278592376No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG80.11730205278592376No Hit
GACTTAAAGGAGAAGAAGGAAGTTGTGGAAGAGGCAGAAAATGGAAGAGA80.11730205278592376No Hit
GGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTT80.11730205278592376No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG80.11730205278592376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.11730205278592376No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC80.11730205278592376No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG70.10263929618768329No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC70.10263929618768329No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT70.10263929618768329No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG70.10263929618768329No Hit
AACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTG70.10263929618768329No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA70.10263929618768329No Hit
CATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAG70.10263929618768329No Hit
GTGTTGCACAAGTACGTTTTGTGACAGGCAATAAAATTTTAAGAATTCTT70.10263929618768329No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT70.10263929618768329No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG70.10263929618768329No Hit
GTCTAGTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATC70.10263929618768329No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC70.10263929618768329No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT70.10263929618768329No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG70.10263929618768329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA70.10263929618768329No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT70.10263929618768329No Hit
ATCTTCAGCTGCCCGCTTGCCCGTAGCTGACTCAGCTTCCTCATCTTCAT70.10263929618768329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)