FastQCFastQC Report
Tue 10 May 2022
EGAF00003676836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642539
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT55690.866717817906773No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC41430.6447857639769726No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT33500.5213691309010037No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT33140.5157663581510228No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG30340.47218923676228214No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC30310.47172233903311706No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA27050.42098611913051187No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA26750.41631714183886115No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG25890.40293274026946224No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG25580.3981081304014231No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT24580.3825448727625872No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG23630.36775977800569304No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT23500.36573655451264436No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG23410.36433586132514917No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA22120.3442592589710508No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG21220.3302523270960984No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT21000.32682841041555455No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA20830.32418265661695245No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA20290.31577849749198106No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG19850.3089306641308932No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG19810.3083081338253398No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC19670.30612927775590276No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT19380.3016159330406403No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG18770.2921223458809504No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA18700.29103291784623186No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT18690.2908772852698435No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA18680.2907216526934552No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT18310.2849632473670859No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC17570.2734464367143473No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC17290.2690887245754732No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT17230.26815492911714306No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC16630.2588169745338415No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC16400.25523742527690924No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA16160.2515022434435887No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC15880.24714453130471456No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC15120.23531645549919925No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT15050.23422702746448074No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA14730.22924678502005327No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA14720.2290911524436649No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC14510.22582286833950937No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA14400.2241109099992374No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG14400.2241109099992374No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC14330.22302148196451888No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG14270.22208768650618874No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT13710.2133722622284406No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC13450.20932581524234325No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG13340.20761385690207132No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG12980.20201108415209035No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA12870.20029912581181844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.19952096292987662No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG12730.1981202697423814No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG12260.19080553865212851No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA11960.18613656136047774No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC11630.18100068633966188No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT11630.18100068633966188No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT11560.17991125830494337No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC11470.17851056511744812No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC11330.1763317090480111No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT11210.17446411813135076No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC11180.1739972204021857No Hit
GCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCT11060.1721296294855254No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC11010.1713514666035836No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC10930.17010640599247673No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG10790.16792754992303968No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA10520.163725470360554No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA10470.1629473074786122No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC10470.1629473074786122No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG10350.16107971656195186No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG10320.1606128188327868No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT10270.159834655950845No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG10250.1595233907980683No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG10190.15858959533973813No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT9800.1525199248605921No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG9790.15236429228420376No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA9720.15127486424948525No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT9700.15096359909670853No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC9680.15065233394393182No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT9670.15049670136754345No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG9630.14987417106199002No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT9610.14956290590921328No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT9360.14567209149950433No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC9250.14396013315923237No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC9240.143804500582844No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG9220.14349323543006728No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG9180.14287070512451383No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG9040.1406918490550768No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT8980.13975805359674667No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT8960.13944678844396993No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC8880.13820172783286308No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG8840.13757919752730963No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG8830.13742356495092126No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA8650.13462217857593078No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA8590.13368838311760065No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT8570.13337711796482393No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG8410.13088699674261017No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCAC8350.12995320128428No Hit
TCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACC8240.12824124294400807No Hit
CCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGG8210.127774345214843No Hit
ACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCAC8190.12746308006206628No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT8180.1273074474856779No Hit
TGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGC8160.1269961823329012No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG8150.12684054975651285No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA8060.1254398565690176No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT8040.1251285914162409No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCA7970.12403916338152236No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT7930.12341663307596892No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC7900.12294973534680384No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT7840.1220159398884737No Hit
GACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCA7740.12045961412459011No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA7720.12014834897181338No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA7710.11999271639542503No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT7690.1196814512426483No Hit
TGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTC7680.11952581866625994No Hit
CCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTT7680.11952581866625994No Hit
CCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCC7610.11843639063154143No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC7570.117813860325988No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT7510.11688006486765784No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC7420.11547937168016259No Hit
CAATCAGGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGC7410.11532373910377425No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT7330.11407867849266738No Hit
TCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC7320.113923045916279No Hit
ACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGC7290.11345614818711394No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG7250.11283361788156049No Hit
TTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCC7140.11112165954128854No Hit
CATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCC7080.1101878640829584No Hit
CTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGC7060.10987659893018167No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA7030.10940970120101659No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA7010.10909843604823986No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG6950.10816464058990972No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG6920.10769774286074464No Hit
TGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACAC6900.10738647770796791No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC6850.10660831482602613No Hit
GGCTTCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGG6830.1062970496732494No Hit
CCCCTGGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATT6800.10583015194408432No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC6770.10536325421491927No Hit
CCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGA6760.10520762163853088No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG6730.1047407239093658No Hit
GGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGC6620.10302876556909385No Hit
CTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAG6530.10162807238159864No Hit
CCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATG6510.10131680722882191No Hit
AGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCA6470.10069427692326847No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG6440.10022737919410339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)