FastQCFastQC Report
Tue 10 May 2022
EGAF00003676841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT951.3884828997369192No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA590.8623209587839813No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA420.6138555977784274No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT380.5553931598947676No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA360.5261619409529378No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG310.45308389359836304No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG310.45308389359836304No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG270.3946214557147033No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT270.3946214557147033No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG260.38000584624378836No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT250.3653902367728734No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT240.35077462730195846No Hit
ATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT230.33615901783104357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.33615901783104357No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT230.33615901783104357No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT220.3215434083601286No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA210.3069277988892137No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC210.3069277988892137No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG200.29231218941829873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2776965799473838No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC180.2630809704764689No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA180.2630809704764689No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT180.2630809704764689No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG170.24846536100555394No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA170.24846536100555394No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC170.24846536100555394No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG170.24846536100555394No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA170.24846536100555394No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC160.233849751534639No Hit
GTACATGGGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACA160.233849751534639No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC150.21923414206372407No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT150.21923414206372407No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG150.21923414206372407No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA150.21923414206372407No Hit
ATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTG150.21923414206372407No Hit
GTACATGGGGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCG140.20461853259280913No Hit
GTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCC140.20461853259280913No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA140.20461853259280913No Hit
GTACATGGGAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTC140.20461853259280913No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG140.20461853259280913No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT130.19000292312189418No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC130.19000292312189418No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130.19000292312189418No Hit
GGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTC130.19000292312189418No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG130.19000292312189418No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT120.17538731365097923No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC120.17538731365097923No Hit
ACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGC120.17538731365097923No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA120.17538731365097923No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC120.17538731365097923No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA110.1607717041800643No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA110.1607717041800643No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT110.1607717041800643No Hit
ATCGCACACTGAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAAC110.1607717041800643No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC110.1607717041800643No Hit
GATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTC110.1607717041800643No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG110.1607717041800643No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC110.1607717041800643No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC110.1607717041800643No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT110.1607717041800643No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC110.1607717041800643No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA110.1607717041800643No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA110.1607717041800643No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT110.1607717041800643No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG110.1607717041800643No Hit
GTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATA100.14615609470914936No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT100.14615609470914936No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA100.14615609470914936No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC100.14615609470914936No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC100.14615609470914936No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA100.14615609470914936No Hit
CTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGC100.14615609470914936No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA100.14615609470914936No Hit
CCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGAT100.14615609470914936No Hit
ACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAG100.14615609470914936No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC100.14615609470914936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.14615609470914936No Hit
ACTTTGTACAACACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGA100.14615609470914936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.14615609470914936No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT100.14615609470914936No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT90.13154048523823444No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC90.13154048523823444No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG90.13154048523823444No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA90.13154048523823444No Hit
CAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTA90.13154048523823444No Hit
TATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATAC90.13154048523823444No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG90.13154048523823444No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC90.13154048523823444No Hit
CGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTC90.13154048523823444No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC90.13154048523823444No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG90.13154048523823444No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATTACACGTGTCCCGC90.13154048523823444No Hit
GTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACG90.13154048523823444No Hit
CCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGC90.13154048523823444No Hit
TTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATC90.13154048523823444No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT90.13154048523823444No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC90.13154048523823444No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA90.13154048523823444No Hit
CCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGC90.13154048523823444No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA80.1169248757673195No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG80.1169248757673195No Hit
ACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCTCGCCGAG80.1169248757673195No Hit
ACGCAGAGTACATGGGAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCC80.1169248757673195No Hit
ACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCG80.1169248757673195No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC80.1169248757673195No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT80.1169248757673195No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT80.1169248757673195No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA80.1169248757673195No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA80.1169248757673195No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA80.1169248757673195No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG80.1169248757673195No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC80.1169248757673195No Hit
GTCTTATCGAGCACAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAAC80.1169248757673195No Hit
AGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG80.1169248757673195No Hit
GCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACT80.1169248757673195No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC80.1169248757673195No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC80.1169248757673195No Hit
TAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTA80.1169248757673195No Hit
GTACAACACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCT80.1169248757673195No Hit
GTACATGGGAAGGTATCGCACACTGAATACATAGGTGTGCGAGGCGAACC80.1169248757673195No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG80.1169248757673195No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA80.1169248757673195No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA80.1169248757673195No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT80.1169248757673195No Hit
GTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCTCGCCGAGCGCGC80.1169248757673195No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA80.1169248757673195No Hit
GCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAAC70.10230926629640456No Hit
CTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCC70.10230926629640456No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT70.10230926629640456No Hit
AGAATATCCTGCAAGGTCGAGGAAGGGTGGCCCGAAGCGGCGGCAGTGGT70.10230926629640456No Hit
CTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT70.10230926629640456No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG70.10230926629640456No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG70.10230926629640456No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA70.10230926629640456No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT70.10230926629640456No Hit
GGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGA70.10230926629640456No Hit
TCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGC70.10230926629640456No Hit
GGCTGATACATTAGCTCGGATGCTATCAGCTTGCGCCTATTATATGCCTG70.10230926629640456No Hit
TATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCT70.10230926629640456No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA70.10230926629640456No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG70.10230926629640456No Hit
GTATCAACGCAGAGTACATGGGAAGAGGGGCGTTGAGTCCGTCCGACTTC70.10230926629640456No Hit
CTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGT70.10230926629640456No Hit
GTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACC70.10230926629640456No Hit
GGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCT70.10230926629640456No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG70.10230926629640456No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT70.10230926629640456No Hit
CCCTGCGACTGCTCGCCGAGCGCGCACGCGCAAGGAGCGCCTGCTTCTTC70.10230926629640456No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT70.10230926629640456No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT70.10230926629640456No Hit
GACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTA70.10230926629640456No Hit
TATCAACGCAGAGTACATGGGAAGAGGGGCGTTGAGTCCGTCCGACTTCA70.10230926629640456No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC70.10230926629640456No Hit
GCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGC70.10230926629640456No Hit
GCAAAAAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAG70.10230926629640456No Hit
GCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGT70.10230926629640456No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT70.10230926629640456No Hit
AGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAG70.10230926629640456No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA70.10230926629640456No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT70.10230926629640456No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC70.10230926629640456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10230926629640456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)