FastQCFastQC Report
Tue 10 May 2022
EGAF00003676842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT640.935399006138556No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA430.6284712072493424No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT380.5553931598947676No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA360.5261619409529378No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG320.467699503069278No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT310.45308389359836304No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA290.42385267465653315No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA290.42385267465653315No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT290.42385267465653315No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG280.40923706518561825No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT280.40923706518561825No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC270.3946214557147033No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT260.38000584624378836No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG240.35077462730195846No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT230.33615901783104357No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC230.33615901783104357No Hit
ATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT230.33615901783104357No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT220.3215434083601286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.3215434083601286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210.3069277988892137No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG200.29231218941829873No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC200.29231218941829873No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG190.2776965799473838No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT190.2776965799473838No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA190.2776965799473838No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG190.2776965799473838No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG180.2630809704764689No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170.24846536100555394No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC170.24846536100555394No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC170.24846536100555394No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG170.24846536100555394No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC160.233849751534639No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT150.21923414206372407No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT150.21923414206372407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.21923414206372407No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG150.21923414206372407No Hit
GTACATGGGAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTC150.21923414206372407No Hit
CCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGC150.21923414206372407No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA140.20461853259280913No Hit
TCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGG140.20461853259280913No Hit
GTATCAACGCAGAGTACATGGGACGAGTCCTACACTATCTTGGATATGAT140.20461853259280913No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC140.20461853259280913No Hit
GTACATGGGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACA140.20461853259280913No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG130.19000292312189418No Hit
GGATTACACGTGTCCCGCCCTACTTGTCGTTAGCTCAGTACCACACGTCT130.19000292312189418No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG130.19000292312189418No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT130.19000292312189418No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC130.19000292312189418No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC130.19000292312189418No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC130.19000292312189418No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA130.19000292312189418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.19000292312189418No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA120.17538731365097923No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA120.17538731365097923No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC120.17538731365097923No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG120.17538731365097923No Hit
GTACATGGGAAGGTATCGCACACTGAATACATAGGTGTGCGAGGCGAACC120.17538731365097923No Hit
ACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCTCGCCGAG110.1607717041800643No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC110.1607717041800643No Hit
ACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAG110.1607717041800643No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA110.1607717041800643No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG110.1607717041800643No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT110.1607717041800643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT110.1607717041800643No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT110.1607717041800643No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC110.1607717041800643No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC110.1607717041800643No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC100.14615609470914936No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA100.14615609470914936No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG100.14615609470914936No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC100.14615609470914936No Hit
GTACATGGGGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCG100.14615609470914936No Hit
TGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGG100.14615609470914936No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA100.14615609470914936No Hit
CAACAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC100.14615609470914936No Hit
GGATAATGGTGGCGACATGCTCCCTCCGCCACCTCCCGTGTCGCGGCCTA100.14615609470914936No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT100.14615609470914936No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA100.14615609470914936No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG100.14615609470914936No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG100.14615609470914936No Hit
GATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGC100.14615609470914936No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT100.14615609470914936No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC100.14615609470914936No Hit
TTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGG100.14615609470914936No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA90.13154048523823444No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC90.13154048523823444No Hit
GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA90.13154048523823444No Hit
GGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCA90.13154048523823444No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA90.13154048523823444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.13154048523823444No Hit
AGCAAAAAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAA90.13154048523823444No Hit
CATTAGCGTATAGGCTGATACATTAGCTCGGATGCTATCAGCTTGCGCCT90.13154048523823444No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT90.13154048523823444No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC90.13154048523823444No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG90.13154048523823444No Hit
GTATCGCACACTGAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAA90.13154048523823444No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT90.13154048523823444No Hit
ATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGT90.13154048523823444No Hit
GCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGT90.13154048523823444No Hit
GCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAG90.13154048523823444No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC90.13154048523823444No Hit
GTACATGGGCTCGGACTCGACACACCGGCCGCGGCGGAGAAAGAAAAGGC90.13154048523823444No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT90.13154048523823444No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT90.13154048523823444No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG90.13154048523823444No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC90.13154048523823444No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA90.13154048523823444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.13154048523823444No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT90.13154048523823444No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT90.13154048523823444No Hit
GAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAG90.13154048523823444No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA90.13154048523823444No Hit
CATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAAT90.13154048523823444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.13154048523823444No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG80.1169248757673195No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG80.1169248757673195No Hit
GGTTACTGTATGCCCCTGTGTTATTGCAGCGTCTCGATTAGGGGGAAACC80.1169248757673195No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC80.1169248757673195No Hit
ACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAG80.1169248757673195No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG80.1169248757673195No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA80.1169248757673195No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA80.1169248757673195No Hit
CTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGC80.1169248757673195No Hit
TATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCT80.1169248757673195No Hit
AGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG80.1169248757673195No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG80.1169248757673195No Hit
CTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGC80.1169248757673195No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA80.1169248757673195No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT80.1169248757673195No Hit
GGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTT80.1169248757673195No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT80.1169248757673195No Hit
GTACATGGGCTGACATTAGCGTATAGGCTGATACATTAGCTCGGATGCTA80.1169248757673195No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG80.1169248757673195No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG80.1169248757673195No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA80.1169248757673195No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA80.1169248757673195No Hit
AATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTT80.1169248757673195No Hit
GAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCG80.1169248757673195No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG80.1169248757673195No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC80.1169248757673195No Hit
TGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTC80.1169248757673195No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT80.1169248757673195No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT70.10230926629640456No Hit
CGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGA70.10230926629640456No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT70.10230926629640456No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG70.10230926629640456No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT70.10230926629640456No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG70.10230926629640456No Hit
GTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCC70.10230926629640456No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG70.10230926629640456No Hit
CGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGC70.10230926629640456No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC70.10230926629640456No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA70.10230926629640456No Hit
ACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGC70.10230926629640456No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA70.10230926629640456No Hit
GTGTCGGCTATGAGCGCACAGTCTCGTCATATAACGATCACGGCAGTGTA70.10230926629640456No Hit
ACGCAGAGTACATGGGACGAGTCCTACACTATCTTGGATATGATATGGCG70.10230926629640456No Hit
GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT70.10230926629640456No Hit
GTACAACACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCT70.10230926629640456No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA70.10230926629640456No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGTCCCTTTGCCCG70.10230926629640456No Hit
CTGAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGT70.10230926629640456No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA70.10230926629640456No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG70.10230926629640456No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA70.10230926629640456No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC70.10230926629640456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)